Plant cell culture techniques are used increasingly in basic research for plant exploitation in industry, including for example, genetic engineering and micropropagation. The rapidly developing role of plant cell culture has necessitated this new edition of a widely acclaimed book. It covers a wide range of methods central to the exploitation of plant cell cultures in fundamental and applied research. This thoroughly revised work retains the combination of giving and explaining the general principles involved with the concise description of specific protocols, with appeal to a broad...

Neurobiology has undergone a revolution with many advances dependent o n new techniques and approaches. This fully updated second edition ref lects the recent growth areas in this subject. Its major themes are th e fractionation and study of cellular and subcellular components of th e nervous system, chemical neuroanatomy, and the measurement of neuron al metabolism in vivo and in vitro. Topics include the neuronal cytosk eleton; isolation of synaptosomes and their subcellular components; as pects of mitochondrial defects in neurones; and synaptic transport pro cesses exemplified by the GABA...

Many investigations into the structure and function of cells and tissu es require the isolation of a particular membrane or subcellular compo nent (organelle). This book covers all the necessary aspects, from the homogenization of cells via a variety of separation techniques, to th e isolation and characterization of peroxisomes and other organelles. Basic separation techniques needed in all cell biology laboratories ar e given and presented in the context of a number of related topics suc h as the labelling of ligands, the kinetics of their internalization a nd analysis of data, and the...

The prediction of the three-dimensional structure of a protein from it s amino acid sequence is a problem faced by an increasing number of bi ological scientists. This book provides a practical guide to making th ese predictions by reviewing strategies of different computer modeling algorithms and highlighting the degree of confidence attributed to th em. Also in the volume are descriptions of the following: the principl es of protein folding; sequence homology and motif searches; predictio n of secondary structure; homology modeling; modeling of antibody comb ining sites; tertiary fold...

Recombinant DNA techniques have revolutionized the isolation and production of antibodies in recent years. This has resulted in rapid changes in how to handle antibodies for research. This new Practical Approach volume responds to this change by assembling, in one volume, protocols which allow the researcher to isolate a new antibody, analyse its properties, format the right antibody molecule or fragment, and produce it in usable quantities. The book is divided into two parts: the first describes the generation and analysis of antibodies and the second covers engineering and production. This...

Protein Structure: A Practical Approach gives general methods for determining protein structure that will be very widely applicable, from the simple electrophoretic, chromatographic, and spectrophotometric techniques to the relatively more complex and sophisticated methods of mass spectrometry and ultracentrifugation. This second edition has been fully updated to embrace the most recent developments in the field, and will be invaluable to all involved in protein research.

Part of a mini-series on the synthesis and processing of proteins, thi s volume details the expression of cloned DNA and RNA templates in all of the major in vivo and in vitro systems.

This new edition is almost a completely new text, with eight of the te n chapters written by new authors. It presents the most reliable metho ds for the major procedures such as one-dimensional polyacrylamide gel electrophoresis, isoelectric focusing, two-dimensional gel electropho resis, preparative gel electrophoresis, and peptide mapping, complete with the latest refinements.

Like the popular first edition, this new edition of Proteolytic Enzymes emphasizes practical aspects of the handling, characterization, inhibition, and use of proteolytic enzymes giving general advice and specific examples. The text and protocols have been thoroughly updated to take account of the advances made in the last 10 years in both the increased understanding of the role of peptidases in many critical cellular processes e.g. apoptosis and new technological developments e.g. in recombinant protein expression, protein sequencing, and structural studies. The topics covered are:...

Macrophages are an important part of the immune response and are characterized by their ability to phagocytose foreign matter. However the difficulties involved in macrophage isolation mean they are some of the body's least explored cells. Macrophage Methodology describes how to isolate moderate to high yields of viable cells from a variety of specific tissue sites under both normal and pathological conditions and then goes on to give protocols for macrophage purification. The third chapter covers techniques used to identify and measure endocytic and phagocytic capabilities using...