With the complete genomes of many organisms now available, and the first draft of the human genome imminent, there is an increasing demand from researchers in a range of disciplines for techniques that will allow them to utilize these resources in their own research. This book provides a comprehensive treatment of the range of methods available for gene and protein expression profiling in a variety of systems. These include large-scale methods, such as cDNA microarrys, serial analysis of gene expression (SAGE) and proteomics, and also methods suitable for non-specialist laboratories, such as...

This volume covers practical important topics in the analysis of protein sequences and structures. It includes comparing amino acid sequences to structures comparing structures to each other, searching information on entire protein families as well as searching with single sequences, how to use the Internet and how to set up and use the SRS molecular biology database management system. Finally, there are chapters on multiple sequence alignment and protein secondary structure prediction. This book will be invaluable to occasional users of these techniques as well as experienced professionals...

This book describes the theory and practical applications of many anal ytical techniques based upon the light absorbing properties of molecul es. Such techniques form the basis of routine tests in chemical, biolo gical, and medical science laboratories. Practical advice is given, fr om experts in the different topics, together with lists of useful lite rature.

Understanding how the body uses specialized immune cells called lympho cytes in fighting both infections and tumors has been central to desig ning new strategies for vaccines and immunotherapy. This book provides practical advice for scientists embarking on the study of lymphocytes in the laboratory, and introduces several new techniques that have re volutionized this field.

The identification and characterization of genes from filamentous fungi has become easier because of the rapid advances that have taken place in molecular biology, including the generation of whole genome sequences. The new challenge facing researchers is to determine the functions of genes and how they contribute to the biology of fungi. To do this it is clear that a wide variety of different experimental techniques will be necessary, ranging from classical genetic analysis and mutant production, right through to cell biology, biochemistry and immunological methods. This book brings together...

Over the past 20 years, the field of biosensor research has had a significant impact in both laboratory research and the commercial sector. Over that period, biosensors have revolutionized the care and management of diabetes and have had important impacts in several other areas of clinical diagnostics. Europe, North America and Asia-Pacific have all seen the rise of small and medium sized companies seeking technical and application niches in the manufacture or use of biosensors. The current activity in both gene and protein 'biochips' can be seen as the latest set of tools that allow users...