Subcellular Fractionation

ISBN-13: 9780199634941 / Angielski / Miękka / 1997 / 360 str.

John Graham; D. Rickwood;Graham

Opis

Many investigations into the structure and function of cells and tissu es require the isolation of a particular membrane or subcellular compo nent (organelle). This book covers all the necessary aspects, from the homogenization of cells via a variety of separation techniques, to th e isolation and characterization of peroxisomes and other organelles. Basic separation techniques needed in all cell biology laboratories ar e given and presented in the context of a number of related topics suc h as the labelling of ligands, the kinetics of their internalization a nd analysis of data, and the electron microscopy of organelles. Subcel lular Fractionation contains expert advice and detailed methodology, a nd will be invaluable to researchers in this important area.