Many investigations into the structure and function of cells and tissu es require the isolation of a particular membrane or subcellular compo nent (organelle). This book covers all the necessary aspects, from the homogenization of cells via a variety of separation techniques, to th e isolation and characterization of peroxisomes and other organelles. Basic separation techniques needed in all cell biology laboratories ar e given and presented in the context of a number of related topics suc h as the labelling of ligands, the kinetics of their internalization a nd analysis of data, and the...

This new edition is almost a completely new text, with eight of the te n chapters written by new authors. It presents the most reliable metho ds for the major procedures such as one-dimensional polyacrylamide gel electrophoresis, isoelectric focusing, two-dimensional gel electropho resis, preparative gel electrophoresis, and peptide mapping, complete with the latest refinements.