ISBN-13: 9780199634392 / Angielski / Miękka / 1997 / 328 str.
Neurobiology has undergone a revolution with many advances dependent o n new techniques and approaches. This fully updated second edition ref lects the recent growth areas in this subject. Its major themes are th e fractionation and study of cellular and subcellular components of th e nervous system, chemical neuroanatomy, and the measurement of neuron al metabolism in vivo and in vitro. Topics include the neuronal cytosk eleton; isolation of synaptosomes and their subcellular components; as pects of mitochondrial defects in neurones; and synaptic transport pro cesses exemplified by the GABA transporter. This is an invaluable prac tical laboratory manual for all researchers in neurobiology; it contai ns step-by-step protocols and a troubleshooting guide, as well as exam ples of experimental data and their interpretation.