Kategoria BISAC: Science >> Research & Methodology

In the last 20 years, research activity using the zebrafish Danio rerio has increased dramatically, due in part to the ease of breeding and raising them, their genetic tractability, embryonic accessibility, and their imaging potential. In Zebrafish: Methods and Protocols, expert researchers provide a collection of experimental techniques for the genetic modification, manipulation, and in vivo live imaging of zebrafish. This volume collects sophisticated and practical techniques, including several not yet widely known, from laboratories around the world. Written in the...

Widely employed for separating and detecting chemicals in solution, separation techniques are most often applied in tandem, subsequently referred to as hyphenated methods. Hyphenated and Alternative Methods of Detection in Chromatography details the development and application of mass spectral detection techniques coupled with gas phase and liquid phase chromatographies. With contributions from experts in a variety of fields, as evidenced by the range of topics, the book describes the advantages and disadvantages of various separation techniques and addresses methods for...

Naturwissenschaftler und Philosophen haben im Lauf der Wissenschaftsgeschichte unterschiedliche Auffassungen vom Hypothesencharakter empirischer Theorien entwickelt. Der Band widmet sich drei verschiedenen Epochen, in denen der Erkenntnisoptimismus erfolgreicher Wissenschaftspraxis auf ein wachsendes Bewusstsein der Grenzen naturwissenschaftlicher Einsicht trifft: der Fruhen Neuzeit (Kopernikus, Kepler, Bacon, Galilei, Descartes, Boyle, Newton, Locke, mit einem Ruckblick auf die mittelalterlichen Autoren Maimonides und Gersonides), dem mechanistischen Weltbild des 19. Jahrhunderts...

Since the discovery of a collagen-degrading protease in the tadpole tail in 1962, matrix metalloproteinase research has led to the discovery of more than twenty distinct vertebrate MMPs, along with a variety of homologues from diverse organisms such as the sea urchin, plants, insects, and nematode worms. Fully updating and adding to the popular first edition, Matrix Metalloproteinase Protocols, Second Edition includes a series of state-of-the-art techniques provided by eminent experts in the field. Beginning with a brief overview of the MMP arena, from how these enzymes fit into...

The ability to detect and quantify apoptosis, to understand its biochemistry, and to identify its regulatory genes and proteins is crucial to biomedical research. In Apoptosis: Methods and Protocols, Second Edition, expert researchers describe the techniques to best investigate the critical steps involved in the apoptotic process. Presented from several different research perspectives, the volume contains sections covering detection of apoptosis, detection of non-apoptotic cell death, modifications of apoptotic proteins during apoptosis, the analysis of its major regulators, as...

In the years since the release of the popular first edition, the field of High Throughput Screening (HTS) has evolved considerably, from a small niche area of study to a major, essential scientific technique. In High Throughput Screening: Methods and Protocols, Second Edition, experts in the field fully update the previous edition to best serve as an introduction to HTS for scientists who are just entering the field, and as a provider of key, significant detail so as to be useful to scientists in established HTS operations. Featuring new screening technologies as well as many well...

Since the development of the concept of proteomics, great progress has granted deeper insight into the cellular network, but the enormous range of protein abundance, dynamics, and interactions leaves many further challenges in the field. In Proteomics: Methods and Protocols, experts in proteomic research have contributed detailed techniques, which present an overview of the contemporary challenges and possibilities in the various areas of proteomics, as well as cutting-edge protocols that provide concrete examples for successful proteome analysis. Written in the clear and concise...

In social anthropology, as in other branches of science, there is a close relationship between research methods and theoretical problems. Advancing theory and shifts in orientation go hand in hand with the development of techniques and mutually influence one another. If the development of modern social anthropology owes much to its established tradition of fieldwork, it is also clear that the procedures that anthropological fieldwork should follow in the laboratory can never be prescribed in absolute terms nor become wholly standardized.

Yet as anthropological analysis is refined,...

As the highly anticipated update to Lipid Second Messengers (CRC Press, 1999), Lipid-Mediating Signaling is a current and comprehensive overview of research methods used in lipid-mediated signal transduction. Pioneering experts provide a much-needed distillation of a decade s worth of advances in research techniques that are pertinent in understanding how lipid-mediated signal transduction ties to pathologic disorders.

Part I describes methods used to identify activities of and assay the expression of different enzymes involved in lipid-mediated...

Since 1997, scientists of different disciplines sharing a deep interest in concepts and knowledge related to evolutionary biology have held the annual Evolutionary Biology Meetings in Marseille in order to discuss their research and promote collaboration. Lately scientists especially focusing on applications have also joined the group.

This book starts with the report of the "12^th Evolutionary Biology Meeting," which gives a general idea of the meeting's epistemological stance. This is followed by 22 chapters, a selection of the most representative contributions, which...

In his 2006 State of the Union speech, President George W. Bush asked the U.S. Congress to prohibit the "most egregious abuses of medical research," such as the "creation of animal-human hybrids." The president's message echoed that of a 2004 report by the President's Council on Bioethics, which recommended that hybrid human-animal embryos be banned by Congress.

Discussions of early interspecies research, in which cells or DNA are interchanged between humans and nonhumans at early stages of development, can often devolve into sweeping statements, colorful imagery, and confusing...

Propelled by the success of the sequencing of the human and many related genomes, molecular and cellular biology has delivered significant scientific breakthroughs. Mathematics (broadly defined) continues to play a major role in this effort, helping to discover the secrets of life by working collaboratively with bench biologists, chemists and physicists.

Because of its outstanding record of interdisciplinary research and training, the IMA was an ideal venue for the 2007-2008 IMA thematic year on Mathematics of Molecular and Cellular Biology. The kickoff event for this thematic year...