Epidermal Cells: Methods and Protocols

ISBN-13: 9781607613794 / Angielski / Twarda / 2009 / 446 str.

Kursad Turksen

Opis

Describes the methods to analyze and manipulate epidermal cell precursors and mature epidermal cells, covering different methods and models for culturing epidermal cells, for enriching very early epidermal progenitors, as well as for studying epidermal cell commitment and differentiation both in vitro and in vivo.

Recenzje

From the reviews of the second edition:

"This book introduces a wide variety of laboratory protocols for isolating and analyzing the differentiation potential of mammalian epidermal cells. ... The book is written for basic science investigators ... . will also be appreciated by clinicians interested in experimental dermatology and dermatopathology. ... This is an important contribution to the literature in adult stem cell biology. ... the theoretical and practical importance of this research and the diversity of topics will help this volume capture the attention of a wide audience of readers." (Bruce A. Fenderson, Doody's Review Service, July, 2010)

Spis treści

1. Isolation of Adult Mouse Stem Keratinocytes Using Magnetic Cell Sorting (MACS) Corina Lorz, Carmen Segrelles, Marina Garín, and Jesús M. Paramio 2. Functional Investigations of Keratinocyte Stem Cells and Progenitors at a Single-Cell Level Using Multiparallel Clonal Microcultures Nicolas O. Fortunel, Pierre Vaigot, Emmanuelle Cadio, and Michèle T. Martin 3. Growth and Stratification of Epithelial Cells in Minimal Culture Conditions Federica Riva, Andrea Casasco, Marco Casasco, Alberto Calligaro, and Antonia Icaro Cornaglia 4. Matched Cultures of Keratinocytes and Fibroblasts Derived from Normal and NER-Deficient Mouse Models Alex Pines and Claude Backendorf 5. Establishment of Spontaneously Immortalised Keratinocyte Lines from Wild-Type and Mutant Mice Julia Reichelt and Ingo Haase 6. Study of Epidermal Differentiation in Human Keratinocytes Cultured in Autocrine Conditions Frédéric Minner, Françoise Herphelin, and Yves Poumay 7. Directed Differentiation of Human Embryonic Stem Cells to Epidermal Progenitors Christian M. Metallo, Lin Ji, Juan J. de Pablo, and Sean P. Palecek 8. Expression and Analysis of Exogenous Proteins in Epidermal Cells Ernest Ho, Wing Y. Chang, and Lina Dagnino 9. Using siRNA Knock-Down in HaCaT Cells to Study Transcriptional Control of Epidermal Proliferation Potential Julie Wells and Xing Dai 10. RNA Interference in Keratinocytes and an Organotypic Model of Human Epidermis Cory L. Simpson, Shin-ichiro Kojima, and Spiro Getsios 11. Scanning for Transcription Factor Binding by a Variant EMSA Igor Gurevich, Carmen Zhang, and Brian J. Aneskievich 12. Chromatin Immunoprecipitation for Identifying Transcription Factor Targets in Keratinocytes Kori Ortt and Satrajit Sinha 13.Gene Expression Profiling of Mouse Epidermal Keratinocytes Ramón García-Escudero and Jesús M. Paramio 14. Analysis of Tissue-Specific Gene Expression Using Laser Capture Microdissection Martin Ruetze, Katrin Lochner, Stefan Gallinat, and Anja Knott 15. Comprehensive Transcriptional Profiling of Human Epidermis, Reconstituted Epidermal Equivalents and Cultured Keratinocytes, Using DNA Microarray Chips Ding-Dar Lee, Jiri Zavadil, Marjana Tomic-Canic, and Miroslav Blumenberg 16. Molecular Profiling of the Epidermis: A Proteomics Approach Jianjun Shen and Susan M. Fischer 17. Detection of Gene Expression in Embryonic Tissues and Stratified Epidermis by In Situ Hybridization Maria I. Morasso 18. Embryonic Mammary Anlagen Analysis Using Immunolabelling of Whole Mounts Heena Panchal, Olivia Wansbury, and Beatrice A. Howard 19. Whole-Mount Assays for Gene Induction and Barrier Formation in the Developing Epidermis Carolyn Byrne, Ariel A. Avilion, Ryan F. O’Shaughnessy, Jonathan C. Welti, and Matthew J. Hardman 20. Tetracycline-Regulated Gene Expression in Transgenic Mouse Epidermis Rose-Anne Romano and Satrajit Sinha 21. A Versatile Murine 3D Organotypic Model to Evaluate Aspects of Wound Healing and Epidermal Organization Eve Kandyba, Malcolm Hodgins, and Patricia Martin 22. Optical and Biochemical Dissection of Connexin and Disease-Linked Connexin Mutants in 3D Organotypic Epidermis Stéphanie Langlois, Jared M. Churko, and Dale W. Laird 23. Cytokine Release in Tissue Engineered Epidermal Equivalents after Prolonged Mechanical Loading Lisette H. Cornelissen*, Debbie Bronneberg, Susan Gibbs, Carlijn V.C. Bouten, and Cees W.J. Oomens 24. Three-Dimensional Human Tissue Models of Wounded Skin Christophe Egles and Jonathan A.

Blurb

In recent years, our ability to understand and manipulate epidermal cells has increased tremendously, opening significant new possibilities in both basic science research and in regenerative medicine, including would healing and transplantation. Epidermal Cells: Methods and Protocols, Second Edition expands upon the popular first edition by bringing together a panel of experienced basic and clinical researchers to describe in step-by-step detail the powerful methods they have developed and optimized to analyze and manipulate epidermal cell precursors and mature epidermal cells. These protocols cover different methods and models for culturing epidermal cells, for enriching very early epidermal progenitors, as well as for studying epidermal cell commitment and differentiation both in vitro and in vivo. Topics of special interest include the derivation, characterization, and utility of epidermal stem cells, mature epidermal cells and their characterization, and applications in regenerative medicine. The protocols follow the successful Methods in Molecular Biology™ series format, providing introductions to their respective topics, lists of the necessary materials and reagents, and notes on troubleshooting and avoiding known pitfalls.

State-of-the-art and highly practical, Epidermal Cells: Methods and Protocols, Second Edition offers experienced and novice investigators alike an invaluable collection of readily reproducible techniques designed to broaden not only our understanding of the biology of epidermal cells, but also their utility in normal tissue homeostasis and regenerative medicine applications.