Protocols for Adult Stem Cells

ISBN-13: 9781607610625 / Angielski / Twarda / 2010 / 220 str.

Irina M. Conboy; David Schaffer; Mary Helen Barcellos-Hoff

Opis

Although embryonic stem cells currently enjoy the public limelight and show great pr- ise for cell based medical therapies, it is the adult stem cells which are responsible for the body's natural ability to fght disease, heal and recover, or fail and succumb to various maladies. The study of mammalian adult stem cells has surged recently, most likely from a maturation of stem cell studies in the classical developmental model organisms and in hematopoeisis. All the tissues of the body examined so far are generated and regenerated from stem cells, it has been an important frst step to adapt or devise new methods to identify and obtain these cells in quantity and purity for further study. Culture techniques have been optimized for managing the growth and differentiation of stem cells in vitro; as some stem cells are pluripotent, often the method is to guide the fate of such cells among the possible differentiation fates. Much of this work, and that in the classical model org- isms, has helped defne the aspects of the stem cell environment or niche that are crucial for both growth and differentiation, and these studies have moved in vivo at increasingly higher resolution. Importantly, the in vivo niche is a current target for bioengineering the matrix and signaling factors. Herein, we present methods for studying six types of mammalian stem cells, m- mary, neural, mesenchymal, endothelial, dendritic, and muscle.

Recenzje

From the reviews:

"This volume in the popular Methods in Molecular Biology series provides a snapshot of research techniques and protocols for identifying and culturing these amazing tissue-specific stem cells. ... The book is written for basic science and clinical researchers interested in stem cell biology, as well as mechanisms of carcinogenesis and malignancy. It also will be valued by biomedical scientists and clinicians interested in cytotechnology and biotechnology. ... The topic is fascinating and clinically relevant. I enjoyed learning about antigen-priming dendritic cells." (Bruce A. Fenderson, Doody's Review Service, August, 2010)

Spis treści

Protocols for Adult Stem Cells Editors Irina Conboy, David Schaffer, Mary Barcellos-Hoff, and Song Li Table of Contents: In Situ Analysis of Cell Populations: Long-Term Label-Retaining Cells Rodrigo Fernandez-Gonzalez, Irineu Illa-Bochaca, Dawne N. Shelton, Bryan E. Welm , Carlos Ortiz-deSolorzano and Mary Helen Barcellos-Hoff Limiting-Dilution Transplantation Assays in Mammary Stem Cell Studies Rodrigo Fernandez-Gonzalez, Irineu Illa-Bochaca1, Dawne N. Shelton, Bryan E. Welm , Carlos Ortiz-deSolorzano and Mary Helen Barcellos-Hoff Use of Stem Cell Markers in Dissociated Mammary Populations Rodrigo Fernandez-Gonzalez,Irineu Illa-Bochaca, Dawne N. Shelton, Bryan E. Welm, Carlos Ortiz-deSolorzano and Mary Helen Barcellos-Hoff Isolation of Adult Hippocampal Neural Progenitors Joseph Peltier, Brandi K. Ormerod, David V. Schaffer In vitro Culture and Analysis of Adult Hippocampal Neural Progenitors Joseph Peltier, Smita Agrawal, Matthew J. Robertson, David V. Schaffer In vivo Analysis of Engrafted Adult Hippocampal Neural Progenitors Matthew J. Robertson, Joseph Peltier, David V. Schaffer Viral Packaging and Transduction of Adult Hippocampal Neural Progenitors Joseph Peltier, David V. Schaffer Culture of Bone Marrow Mesenchymal Stem Cells on Engineered Matrix Jennifer S. Park, Craig Hashi and Song Li Preparation and analysis of endothelial progenitor cells associated with angiogenesis Steve Khachi, Yiqian Zhu, and Guo-Yuan Yang Preparation of adult muscle fiber associated stem/precursor cells Michael J. Conboy and Irina M. Conboy Immuno-analysis and FACS sorting of adult muscle fiber associated stem/precursor cells Michael J. Conboy, Massimiliano Cerletti, Amy J. Wagers and Irina M. Conboy Altered expression of NF-kappa B in ex vivo differentiated dendritic cells from the aged subjects: Implications in immunotherapy Anshu Agrawal Preparation of collagen-coated gels that maximize in vitro myogenesis of stem cells by matching the lateral elasticity of in vivo muscle Tathagata Chaudhuri, Florian Rehfeldt, H. Lee Sweeney and Dennis E. Discher

O autorze

Li Song is a member of the Theory Committee of the Chinese Artists Association, former editor-in-chief of Fine Art and Chinese Fine Art magazines, and deputy director of the Yanhuang Art Museum. He has published widely on the history of Chinese art.

Blurb

The study of adult stem cells has surged in recent years. Because they are responsible for the body’s natural ability to fight diseases, heal and recover, or fail and succumb to various maladies, it has become increasingly important to adapt or devise new methods to identify and obtain these cells in quantity and purity for further study. In Protocols for Adult Stem Cells, expert researchers present a variety of methods for studying five types of clinically-relevant mammalian stems cells: mammary, nerve, skeletal muscle, endothelial and mesenchymal. Culture techniques have been optimized for managing the growth and differentiation of stem cells in vitro; as some stem cells are pluripotent, often the method is to guide the fate of such cells among the possible differentiation fates. Chapters include information that will assist researchers in obtaining, characterizing and studying these cells or adapting them to the stem cells of choice. Composed in the highly successful Methods in Molecular Biology™ series format, each chapter contains a brief introduction, step-by-step methods, a list of necessary materials, and a Notes section which shares tips on troubleshooting and avoiding known pitfalls.

Critical and cutting edge, Protocols for Adult Stem Cells is an essential guide which provides groundbreaking and novel techniques certain to redefine the field of stem cell biology.