Autophagy and phagocytosis are distinct yet partially morphologically similar processes. In Autophagosome and Phagosome, authoritative scientists present easy-to-follow methods on autophagy, a rapidly growing field with a need for standards of assessment, and phagocytosis, a relatively mature field which could benefit greatly from updated methods, in order to prompt further explorations of their similarities and differences. The methods on autophagy allow the reader to find appropriate techniques to identify, monitor, and quantify autophagic processes, while the methods devoted to...
Autophagy and phagocytosis are distinct yet partially morphologically similar processes. In Autophagosome and Phagosome, authoritative scientists p...
Many questions related to stem cell properties and neural stem cell lineage and differentiation still linger. This second edition revises and expands upon the successful first edition in order to provide the most current, cutting-edge methods of today for the scientists working to answer these questions. The use of these step-by-step, readily reproducible laboratory protocols will allow investigators to produce pure populations that can serve as a means of understanding the biology of neural stem cells and adapting them for transplantation into disease models. This is an excellent source...
Many questions related to stem cell properties and neural stem cell lineage and differentiation still linger. This second edition revises and expan...
Agrobacterium tumefaciens is a soil bacterium that for more than a century has been known as a pathogen causing the plant crown gall disease. Unlike many other pathogens, Agrobacterium has the ability to deliver DNA to plant cells and permanently alter the plant genome. The discovery of this unique feature 30 years ago has provided plant scientists with a powerful tool to genetically transform plants for both basic research purposes and for agric- tural development. Compared to physical transformation methods such as particle bomba- ment or electroporation, Agrobacterium-mediated DNA delivery...
Agrobacterium tumefaciens is a soil bacterium that for more than a century has been known as a pathogen causing the plant crown gall disease. Unlike m...
In this book, a select group of researchers has contributed their state-of-the-art methodologies on protein profiling and identification of disease biomarkers in tissues, microdissected cells and body fluids. The book integrates biochemistry, pathology, analytical technology, bioinformatics, and proteome informatics. Experimental approaches are thoroughly detailed and explained through a step-by-step instructional format that ensures successful results.
In this book, a select group of researchers has contributed their state-of-the-art methodologies on protein profiling and identification of disease...
Following the considerable success of the first edition of Plant Virology Protocols, this exciting new edition covers the many new techniques that are now applied to the examination and understanding of plant viruses. Each section presents the most novel methods and step-by-step reproducible laboratory protocols to allow researchers more effective approaches to study plant viruses. This updated book will prove indispensable to laboratory investigators studying plant viruses.
Following the considerable success of the first edition of Plant Virology Protocols, this exciting new edition covers the many new techniques that ...
Drosophila melanogaster has been the model system of choice for many inv- tigators over the past hundred years. Due to its long-standing tradition as a model organism, many techniques used in Drosophila have been established and c- tinue to be developed to answer a variety of scientific questions. The recent inv- tion of techniques allowing the knock-down of genes by RNA interference and gene replacement by homologous recombination are two important demonst- tions of the ongoing efforts to broaden the technical repertoire at hand for researchers working with Drosophila. This volume is a...
Drosophila melanogaster has been the model system of choice for many inv- tigators over the past hundred years. Due to its long-standing tradition as ...
The objective of this volume is to provide readers with a current view of all aspects of the 'pipeline' that takes protein targets to structures and how these have been optimised. This volume includes chapters describing, in-depth, the individual steps in the Structural Genomics pipeline, as well as less detailed overviews of individual Structural Genomics initiatives. It is the first book of protocols to cover techniques in a new and emerging field.
The objective of this volume is to provide readers with a current view of all aspects of the 'pipeline' that takes protein targets to structures an...
Molecular biology has transformed prenatal diagnosis because it permits an accurate diagnosis to be made from very small quantities of fetal material, even single cells. Although the latter permits the analysis of preimplantation embryos and, perhaps in the future, the analysis of fetal cells enriched from maternal blood, a major current focus is to facilitate rapid, cost-effective diagnoses. In this manner, the use of fluorescence in situ hybridization (FISH) or polymerase chain reaction (PCR)-based approaches on uncultured amniocytes or chorionic villi already permits a rapid assessment of...
Molecular biology has transformed prenatal diagnosis because it permits an accurate diagnosis to be made from very small quantities of fetal material,...
Neutrophil Methods and Protocols provides a concise set of protocols for assessing basic neutrophil functions, investigating specialized areas in neutrophil research, and completing step-by-step diagnostic assays of common neutrophil disorders. Each of the protocols is written by leading researchers in the field and includes hints for success, as well as guidance for troubleshooting. Parts 1 & 2 provide an overview of neutrophils the methods most commonly used for isolating them from humans and other animal species. Part 3 details methods for investigating adhesion, chemotaxis,...
Neutrophil Methods and Protocols provides a concise set of protocols for assessing basic neutrophil functions, investigating specialized areas in n...
Now in two volumes, this completely updated and expanded edition of Embryonic Stem Cells: Methods and Protocols provides a diverse collection of readily reproducible cellular and molecular protocols for the manipulation of nonhuman embryonic stem cells. Volume two, Embryonic Stem Cell Protocols: Differentiation Models, Second Edition, covers state-of-the-art methods for deriving many types of differentiating cells from ES cells. The first volume, Embryonic Stem Cell Protocols: Isolation and Characterization, Second Edition, provides a diverse collection of readily reproducible cellular and...
Now in two volumes, this completely updated and expanded edition of Embryonic Stem Cells: Methods and Protocols provides a diverse collection of readi...
