Clinical Proteomics: Methods and Protocols

ISBN-13: 9781617377990 / Angielski / Miękka / 2010 / 408 str.

Antonia Vlahou

Opis

In this book, a select group of researchers has contributed their state-of-the-art methodologies on protein profiling and identification of disease biomarkers in tissues, microdissected cells and body fluids. The book integrates biochemistry, pathology, analytical technology, bioinformatics, and proteome informatics. Experimental approaches are thoroughly detailed and explained through a step-by-step instructional format that ensures successful results.

Recenzje

From the reviews:

"Clinical proteomics is a fledgling field that is struggling to define its standards and whose underlying technologies continue to evolve at a very rapid pace. ... This multiauthored book provides state-of-the-art information and practical protocols on these topics and many other related areas. ... provides a wealth of background information and underlying principles. ... Overall, this is an excellent book whose natural readership includes both newcomers to the field and seasoned biomarker hunters who wish to update their knowledge or explore new techniques." (Marco Crescenzi, Annali dell'Istituto Superiore di Sanita, Vol. 44 (3), November, 2008)

Spis treści

Contents Preface……………………………………………………………………………………………………………........... Contributors……………………………………………………………………………………………………………… 1 Overview and Introduction to Clinical Proteomics Young-Ki Paik, Hoguen Kim, Eun-Young Lee, Min-Seok Kwon and Sang Yun Cho..................... Specimen Collection for Clinical Proteomics 2 Specimen collection and handling: Standardization of blood sample collection Harald Tammen................................................................................................................................ 3 Tissue Sample Collection for Proteomics Analysis Jose L Diaz, Lisa H Cazares and O. John Semmes …………………………………………………. Clinical Proteomics by 2DE and direct MALDI/SELDI MS profiling 4 Protein profiling of Human Plasma Samples by Two-Dimensional Electrophoresis Sang Yun Cho, Eun-Young Lee, Hye-Young Kim, Min-Jung Kang, Hyoung-Joo Lee, Hoguen Kim and Young-Ki Paik …………………………………………………………………………….. 5 Analysis of Laser Capture Microdissected Cells by 2-Dimensional Gel Electrophoresis Daohai Zhang and Evelyn Siew-Chuan Koay …………………………………………........................... 6 Optimizing the Difference Gel Electrophoresis (DIGE) technology David B. Friedman and Kathryn S. Lilley ……………………………………………………………….. 7 MALDI/SELDI protein profiling of serum for the identification of cancer biomarkers Lisa H Cazares, Jose I Diaz, Rick R Drake and O John Semmes………………………………………. 8 Urine sample preparation and protein profiling by two-dimensional electrophoresis and matrix assisted laser desorption ionization time-of-flight mass spectrometry. Panagiotis G. Zerefos and A. Vlahou ……………………………………………………………………….. 9 Combining Laser Capture Microdissection and proteomics techniques. Review Dana Mustafa, Johan M. Kros, and Theo Luider………………………………………………………….. Clinical proteomics by LC-MS approaches 10 Comparison of Protein Expression by Isotope-Coded Affinity Tag Labelling. Zhen Xiao and Timothy D. Veenstra…chr(133P>Zhen Xiao, Thomas P. Conrads, George R. Beck Jr. and Timothy D. Veenstra……………………. Clinical Proteomics and Antibody Arrays 14 Miniaturised parallelized sandwich immunoassays Hsin-Yun Hsu, Silke Wittemann and Thomas O. Joos…………………………………………………… 15 Dissecting cancer serum protein profiles using antibody arrays. Marta Sanchez-Carbayo……………………………………………………………………………………. Statistics and Bioinformatics in Clinical proteomics data analysis 16 2D-PAGE Maps Analysis Emilio Marengo, Elisa Robotti, Marco Bodda……………………………………………………………... 17 Finding the significant markers: statistical analysis of proteomics data. Sebastien Christian Carpentier, Bart Panis, Rony Swennen and Jeroen Lammertyn 18 Web-Based Tools for Protein Classification. Costas D. Paliakasis, Ioannis Michalopoulos and Sophia

Blurb

The field of clinical proteomics has rapidly evolved during the past few years and is continuously growing as new methodologies and technologies emerge. In Clinical Proteomics, a select group of leading researchers has contributed their state-of-the-art methodologies on protein profiling and identification of disease biomarkers in tissues, microdissected cells and body fluids. Experimental approaches involving the application of two-dimensional electrophoresis, multi-dimensional liquid chromatography, SELDI/MALDI mass spectrometry and protein arrays, as well as the bioinformatic and statistical tools pertinent to the analysis of proteomics data, are thoroughly detailed in these readily reproducible methods and protocols, explained through the step-by-step instructional format of the highly successful Methods in Molecular Biology™ series.

With its multi-disciplinary approach, Clinical Proteomics provides integrated, cutting-edge techniques sure to be invaluable to a wide range of researchers including clinicians, molecular biologists, chemists, bioinformaticians and computational biologists.