Kategoria BISAC: Science >> Life Sciences - Biochemistry

The authoritative guide on protein purification--now completely updated and revised

Since the Second Edition of Protein Purification was published in 1998, the sequencing of the human genome and other developments in bioscience have dramatically changed the landscape of protein research. This new edition addresses these developments, featuring a wealth of new topics and several chapters rewritten from scratch.

Leading experts in the field cover all major biochemical separation methods for proteins in use today, providing professionals in biochemistry, organic...

Recent Advances in Polyphenol Research

Volume 2

Edited by Santos-Buelga, Escribano-Bailon and Lattanzio

Plant phenolics are secondary metabolites that constitute one of the most common and widespread groups of substances in plants. Polyphenols have a large and diverse array of beneficial effects on both plants and animals. For example they are famous as antioxidants, hormones, constituents of essential oils and natural neurotransmitters.

Sponsored by Groupe Polyphenols, this publication, which is the second volume in this ground-breaking series, is edited...

The field of plant nitrogen metabolism continues to be a compelling focus for basic research activities because there is a strong demand for immediate solutions, particularly in key areas, such as improving plant nitrogen use efficiency, which are crucial to future agricultural sustainability and the future economic success of agriculture.

The 13 reviews which comprise this excellent and carefully edited new volume bring together the expertise and enthusiasm of an international team of leading researchers. Topics covered include nitrogen sensing and signalling, uptake and membrane systems,...

Bringing the power of biochemical analysis to toxicology, this modern reference explains genotoxicity at the molecular level, showing the links between a DNA lesion and the resulting cellular or organismic response. Clearly divided into two main sections, Part 1 focuses on selected examples of important DNA lesions and their biological impact, while the second part covers current advances in assessing and predicting the genotoxic effects of chemicals, taking into account the biological responses mediated by the DNA repair, replication and transcription machineries.
A ready reference for...

"Coming to a conclusion, this wonderful, informative and very interesting book presents an excellent overview of small volatile organic compounds and their role in our life and environment. Really fascinating is the entirety of scientific disciplines which were addressed by this book." -Flavour and Fragrance Journal, 2011

..". this book deserves to be a well-used reference in the library of any laboratory specialising in VOC." -Chemistry World, 2011

Volatile compounds are molecules with a relatively low molecular weight allowing for an efficient evaporation into...

Proteases or proteinases have a wide application in different industrial processes including cheese production, meat tenderization, production of protein hydrolysates, silver recovery and silk; in many other fields including biotechnological, baking, textiles, pharmaceutical and leather industries and also, as an additive to detergents and in bioremediation procedures. These enzymes are present in plants, animals and microorganisms (protozoa, bacteria, fungus and some types of algae and viruses). Considering the wide applications of these enzymes, it is necessary to know their functions,...

Advanced Glycation End-Products: Sources and Effects attempts to describe the role of advanced glycation end-products, particularly dietary advanced glycation end-products, in the pathological expansion of adipose tissue as well as their implications in the development of obesity associated complications. The effects of advanced glycation end-products on vasculature and their pathological implications are also described, focusing on the epigenetic mechanisms (particularly that of miRNA) modulating their effects. The authors summarize the information available on the impact of hyperglycemia on...

The book focuses on the regulation of protein function and biological activity by the post-translational modification known as acetylation. Chapters detail a wide range of topics in protein acetylation dealing with pathways relevant to cellular homeostasis, gene expression, and DNA metabolism. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. ...

This second edition volume expands all chapters of the previous edition, which have been enhanced to cover the most recent developments, the current state of method research, and applications. Additional protocols were added to examine lipid-protein interactions by mass spectrometry, to use protein microarrays to investigate large sets of various proteins, to study membrane protein dynamics by UV resonance Raman spectroscopy, to analyze peptide-induced pore formation in membranes, and to investigate folding and insertion of membrane proteins. Written in the highly successful Methods in...

New insights into modern medicine and systems biology are enabled by innovative protocols and advanced technologies in mass spectrometry-based proteomics. This volume details new pipelines, workflows, and ways to process data that allow for new frontiers in proteomics to be pushed forward. With applications to biomarker discovery, interactions between proteins, between biological systems, dynamics of post-translational modifications among others, new protocols have been developed and iteratively refined to probe the endless complexity of the proteome in ever greater details. This volume deals...

This detailed volume explores the NADPH oxidase family of enzymes in human physiology and genetic disease, in which early discoveries represent prime examples of the finest translational "from bed to bench and back" studies. Methods are included for testing assembly and function of multicomponent oxidase complexes and for analyzing reactive oxygen species (ROS) generation in different systems by various means, while addressing pitfalls of ROS probes currently being used, as well as protocols on NADPH oxidase regulation and their function in cells. Written in the highly successful Methods in...

This book compiles key protocols instrumental to the study of high-throughput protein production and purification which have been refined and simplified over the years and are now ready to be transferred to any laboratory. Beginning with a section covering general procedures for high-throughput protein production, the volume continues with high-throughput protocols adapted to the production of specific protein families, as well as an extensive section on protocols combining high-throughput protein production and their micro-characterization. Written for the highly successful Methods in...

This volume explores techniques used to detect lipids attached to proteins, to analyze the function of lipid modifications, and to characterize the enzymes that add and remove lipids from proteins. The book is organized into seven parts: Part One describes chemically-based strategies to identify substrates for protein lipidation that can be applied to individual proteins or globally using proteomics. Part Two focuses on the enzymes that remove fatty acids from proteins and provides methods to monitor protein biogenesis and palmitate turnover. Part Three addresses biochemical and cellular...

This volume explores the latest synthetic procedures for producing receptor-specific retinoids and rexinoids, molecular biology methods, and new technologies to demonstrate the therapeutic activities of molecules. The chapters in this book cover topics such as lentiviral delivery of shRNA constructs into acute promyelocytic leukemia cells for ATRA induced differentiation and autophagy; methods to analyze RAR signaling in colorectal cancer cells; differentiation of primary myoblasts by using RXR agonist; methodology for analyzing effects of retinoid treatment on nervous system development and...

This book provides detailed protocols and untold tips and tricks regarding the most well-known examples of proximity labeling methods, in which the protein of interest is genetically fused to or labeled with an enzyme that can generate short-lived reactive species to non-specifically label molecules within a certain radius of up to twenty nanometers. Beginning with peroxidase-based proximity labeling methods, the volume continues with BioID, proximity labeling methods that describe the proximity ligation assay to detect RNA-DNA interactions, UV cross-linking to demonstrate RNA-protein...

The accessible introduction to biomaterials and their applications in tissue replacement, medical devices, and more

Molecular and cell biology is being increasingly integrated into the search for high-performance biomaterials and biomedical devices, transforming a formerly engineering- and materials science-based field into a truly interdisciplinary area of investigation. Biomimetic, Bioresponsive, and Bioactive Materials presents a comprehensive introduction to biomaterials, discussing how they are selected, designed, and modified for integration with living tissue and how...

The biological world operates on a multitude of scales - from molecules to cells to tissues to organisms to ecosystems. Throughout all these levels runs a common thread: the communication and onward passage of information - from cell to cell, from organism to organism and, ultimately, from generation to generation. But how does this information - no more than a static repository of data - come alive to govern the processes that constitute life? The answer lies in the concerted action of molecular components which cooperate through a series of carefully-regulated processes to bring the...

The volume explores methods for isolation and characterization of lipids, and their production using various oleaginous organisms. The protocols presented in the book consider sugars derived from different substrates including chemically pre-treated agricultural residues, industrial residues containing lignin, food wastes, and industrial waste water in an approachable format. The book also discusses applications that use oleaginous organisms to transform substrates into a variety of products including bio-crude, high value fatty acids, biofuels such as biodiesel, neutral lipids, volatile...

This book covers strategies to improve cell permeability, intestinal permeability, and metabolic stability, which are the typical liabilities associated with cyclic peptides, to enhance protein-protein recognition, and to build upon nature's cyclic peptides and macrocycles. Chapters also cover key peptide screening and display strategies, as well as important synthetic approaches towards cyclic and helical peptides. Cyclic peptides have become of significant importance as chemical tools in biology and drug discovery, since this class of chemicals has become a credible alternative source of...