Neutrophil Methods and Protocols

ISBN-13: 9781617377792 / Angielski / Miękka / 2010 / 537 str.

Mark T. Quinn; Frank R. DeLeo; Gary M. Bokoch

Opis

Neutrophil Methods and Protocols provides a concise set of protocols for assessing basic neutrophil functions, investigating specialized areas in neutrophil research, and completing step-by-step diagnostic assays of common neutrophil disorders. Each of the protocols is written by leading researchers in the field and includes hints for success, as well as guidance for troubleshooting. Parts 1 & 2 provide an overview of neutrophils the methods most commonly used for isolating them from humans and other animal species. Part 3 details methods for investigating adhesion, chemotaxis, phagocytosis and bacterial activity. Part 4 presents protocols measuring gene expression in neutrophils, and Part 5 concludes with assays for diagnosing the most common neutrophil disorders. Both basic scientists and clinicians will find a collection of this caliber to be an invaluable aid in their work with neutrophils.

Recenzje

From the reviews:

"...a carefully compiled collection of protocols that allow for study of many aspects of the neutrophil." -Doody's Book Review, Weighted Numerical Score:85-3 Stars

"This excellent book is one of the latest in the 'Methods in Molecular Biology' series ... . it comes beautifully presented and bound in a hardback cover and would grace any bookshelf. I would urge all self-respecting laboratories working with neutrophils - whether in research or diagnostics - to purchase one immediately. ... I was impressed by this book's comprehensive provision of protocols, addressing all aspects of neutrophil function and physiology, and including an excellent section on diagnosis of both inherited and non-inherited neutrophil disorders." (Lynn Morgan, Immunology News, May, 2008)

Spis treści

Neutrophils Methods and Protocols Edited by Mark T. Quinn Frank R. DeLeo Gary M. Bokoch Contents Dedication i Preface ii Contributors v PART I: NEUTROPHILS: AN OVERVIEW 1. The Role of Neutrophils in the Immune System: An Overview Harry L. Malech PART II: NEUTROPHIL ISOLATION AND SUBCELLULAR FRACTIONATION 2. Isolation of Human Neutrophils From Venous Blood William M. Nauseef 3. Neutrophil Isolation From Non-Human Species Daniel W. Siemsen, Igor A. Schepetkin, Lilia N. Kirpotina, Benfang Lei, and Mark T. Quinn 4. Subcellular Fractionation of Human Neutrophils and Analysis of Subcellular Markers Lene Udby and Niels Borregaard PART III. BIOCHEMISTRY, BIOLOGY AND SIGNAL TRANSDUCTION OF NEUTROPHILS 5. Rac and Rap GTPase Activation Assays Ulla G. Knaus, Alison Bamberg, and Gary M. Bokoch 6. Measurement of Phospholipid Metabolism in Intact Neutrophils Susan Sergeant and Linda C. McPhail 7. Analysis of Protein Phosphorylation in Human Neutrophils Jamel El-Benna and Pham My-Chan Dang 8. Mitogen-Activated Protein Kinase Assays Martine Torres 9. Transduction of Proteins into Intact Neutrophils Tieming Zhao and Gary M. Bokoch 10. Optical Methods for the Measurement and Manipulation of Cytosolic Free Calcium in Neutrophils Esther J. Hillson, Sharon Dewitt, and Maurice B. Hallett 11. Analysis of Electrophysiological Properties and Responses of Neutrophils Deri Morgan and Thomas E. DeCoursey 12. Analysis of Neutrophil Apoptosis Emma L. Taylor, Adriano G. Rossi, Ian Dransfield, and Simon P. Hart PART IV. NEUTROPHIL ADHESION AND CHEMOTAXIS 13. Optical and Fluorescence Detection of Neutrophil Integrin Activation Ulrich Y. Schaff, Melissa R. Sarantos, Harold Ting, and Scott I. Simon 14. Analysis of Neutrophil Polarization and Chemotaxis Mary A. Lokuta, Paul A. Nuzzi, and Anna Huttenlocher 15. Quantifying and Localizing Actin Free Barbed Ends in Neutrophils Michael Glogauer 16. Measurement of Neutrophil Adhesion Under Conditions Mimicking Blood Flow Mark A. Jutila, Bruce Walcheck, Robert Bargatze, and Aiyappa Palecanda 17. Model Systems to Investigate Neutrophil Adhesion and Chemotaxis Nancy A. Louis, Eric Campbell, and Sean P. Colgan PART V. NEUTROPHIL PHAGOCYTOSIS AND BACTERICIDAL ACTIVITY 18. Immunofluorescence and Confocal Microscopy of Neutrophils Lee-Ann H. Allen 19. Assessment of Phagosome Formation and Maturation by Fluorescence Microscopy Benjamin E. Steinberg and Sergio Grinstein 20. Analysis of Neutrophil Membrane Traffic During Phagocytosis Per Lönnbro, Pontus Nordenfelt, and Hans Tapper 21. Analysis of Neutrophil Bactericidal Activity Jessie N. Green, Christine C. Winterbourn, and Mark B. Hampton 22. A Skin Chamber Technique as a Human Model for Studies of Aseptic Inflammatory Reactions Per Follin and Claes Dahlgren PART VI. NADPH OXIDASE AND PRODUCTION OF REACTIVE OXYGEN SPECIES 23. Measurement of Respiratory Burst Products Generated by Professional Phagocytes Claes Dahlgren, Anna Karlsson, and Johan Bylund 24. The K-562 Cell Model for Analysis of Neutrophil NADPH Oxidase Function Thomas L. Leto, Mark C. Lavigne, Neda Homoyounpour, Kristen Lekstrom, Gilda Linton, Harry L. Malech, and Isabelle de Mendez 25. Cell-free Assays: The Reductionist Approach to the Study of NADPH Oxidase Assembly Shahar Molshanski-Mor, Ariel Mizrahi, Yelena Ugolev, Iris Dahan, Yevgeny Berdichevsky, and Edgar Pick 26. Immunoaffinity Purification of Human Phagocyte Flavocytochrome b and Analysis