Hands-on researchers review the principles behind successful miniaturization and describe the key techniques for miniaturizing large-scale biochemical and bioanalytical methods for microchip analysis. The authors cover not only the most popular methods for the fabrication of microchips (photolithography, laser ablation, and soft lithography), but also microfluidic techniques for such bioanalytical assays and bioprocesses as DNA analysis, PCR, immunoassays, and cell reactors. Highlights include PCR on a microchip, microscale cell culturing, and the study of cellular processes on a microchip....
Hands-on researchers review the principles behind successful miniaturization and describe the key techniques for miniaturizing large-scale biochemical...
The first edition of this book, published in 1999 and called DNA Repair Protocols: Eukaryotic Systems, brought together laboratory-based methods for studying DNA damage and repair in diverse eukaryotes: namely, two kinds of yeast, a nematode, a fruit fly, a toad, three different plants, and human and murine cells. This second edition of DNA Repair Protocols covers mammalian cells only and hence its new subtitle, Mammalian Systems. There are two reasons for this fresh emphasis, both of them pragmatic: to cater to the interests of what is now a largely mammalocentric DNA repair field, and to...
The first edition of this book, published in 1999 and called DNA Repair Protocols: Eukaryotic Systems, brought together laboratory-based methods for s...
Cancer Genomics and Proteomics provides a compendium of techniques and applications in gene identification and function. The approaches described in detail are state-of-the art and can be tailored to individual ongoing or planned research projects. This volume is a valuable laboratory resource for designing experiments to identify and analyze genes that are relevant to complex biological phenomena.
Cancer Genomics and Proteomics provides a compendium of techniques and applications in gene identification and function. The approaches described i...
Intracellular checkpoint controls constitute a network of signal transd- tion pathways that protect cells from external stresses and internal errors. Ext- nal stresses can be generated by the continuous assault of DNA-damaging agents, such as environmental mutagens, ultraviolet (UV) light, ionizing radiation, or the reactive oxygen species that can arise during normal cellular metabolism. In response to any of these assaults on the integrity of the genome, the activation of the network of checkpoint control pathways can lead to diverse cellular responses, such as cell cycle arrest, DNA...
Intracellular checkpoint controls constitute a network of signal transd- tion pathways that protect cells from external stresses and internal errors. ...
Now in two volumes, this completely updated and expanded edition of Embryonic Stem Cells: Methods and Protocols provides a diverse collection of readily reproducible cellular and molecular protocols for the manipulation of nonhuman embryonic stem cells. Volume one, Embryonic Stem Cell Protocols: Isolation and Characterization, Second Edition, provides a diverse collection of readily reproducible cellular and molecular protocols for the isolation, maintenance, and characterization of embryonic stem cells. The second volume, Embryonic Stem Cell Protocols: Differentiation Models, Second Edition,...
Now in two volumes, this completely updated and expanded edition of Embryonic Stem Cells: Methods and Protocols provides a diverse collection of readi...
A cutting-edge collection of basic and state-of-the-art methods optimized for investigating the molecular biology of this class of retrovirus. These readily reproducible techniques range from methods for the isolation and detection of human retroviruses to cutting-edge methods for exploring the interplay between the viruses and the host. Here, the researcher will find up-to-date techniques for the isolation and propagation of HIV, HTLV, and foamy virus from a variety of sources. There are also assays for determining the cell tropism of HIV-1, the coreceptor usage of HIV-1, and human gene...
A cutting-edge collection of basic and state-of-the-art methods optimized for investigating the molecular biology of this class of retrovirus. These r...
In this first book dedicated entirely to the ELISPOT, a critical enzyme-linked immunospot assay used widely in biomedical research, recognized experts with first-hand experience detail how to design, perform, and analyze these assays. The readily reproducible techniques they provide cover a wide variety of topics, including the use of membrane-backed plates, the standardization and validation procedures, the removal of cells from ELISPOT plates, cell separation techniques, and the quantification of ELISPOT data. There are also numerous ELISPOT applications involving animal models, human...
In this first book dedicated entirely to the ELISPOT, a critical enzyme-linked immunospot assay used widely in biomedical research, recognized experts...
The aim of Apoptosis and Cancer is to describe the performance of contemporary techniques for studying the biology of apoptosis and its role in cancer. The protocols described will aid both the academic laboratory interested in further characterizing the mechanisms of apoptosis, as well as the industry laboratory, aimed at identifying new target molecules or screening for new compounds with potential clinical use.
The aim of Apoptosis and Cancer is to describe the performance of contemporary techniques for studying the biology of apoptosis and its role in can...
Plant-Pathogen Interactions: Methods and Protocols provides key methods, approaches, and strategies to dissect the plant defense response. Addressing methods to identify and characterize plant resistance genes as well as pathogen-associated molecules that trigger the plant defense response, this volume creates a better understanding of the interactions between pathogens and their hosts, which will help to develop better methods for disease control in plants and animals. Plant-Pathogen Interactions: Methods and Protocols reviews methods for engineering resistance to plant viruses, the...
Plant-Pathogen Interactions: Methods and Protocols provides key methods, approaches, and strategies to dissect the plant defense response. Addressing ...
In the first edition of Calcium Signaling Protocols I began by writing "The regula- 2+ tion of intracellular Ca is a common theme presented in many papers over the last 20 2+ or so years and the description of the Ca -sensitive indicator dye fura-2 in 1985 resulted in a massive increase in these types of studies. " This statement is as true in 2005 as it was in 1999, but 20 or so years is now 30 years There has been some reorganization of the volume such that there are now 22 ch- ters including five new ones, all written by experts in their field. These new chapters 2+ include use of the...
In the first edition of Calcium Signaling Protocols I began by writing "The regula- 2+ tion of intracellular Ca is a common theme presented in many pa...
