Apoptosis and Cancer: Methods and Protocols

ISBN-13: 9781617375828 / Angielski / Miękka / 2010 / 266 str.

Gil Mor; Ayesha Alvero

Opis

The aim of Apoptosis and Cancer is to describe the performance of contemporary techniques for studying the biology of apoptosis and its role in cancer. The protocols described will aid both the academic laboratory interested in further characterizing the mechanisms of apoptosis, as well as the industry laboratory, aimed at identifying new target molecules or screening for new compounds with potential clinical use.

Recenzje

From the reviews:

"Divided among 17 chapters, the condensed information about apoptosis, its pathways and the performance of progressive techniques for apoptosis assessment are presented. ... The valuable and most appreciated part of each chapter are notes - the reader will find here the plethora of hints, the real grain of wisdom, virtual wizard that saves a lot of struggling time at benchtop. This book belongs to the category of must-have books." (J. Sedlák, Neoplasma, September, 2008)

Spis treści

APOPTOSIS AND CANCER Methods and Protocols Methods in Molecular Medicine Editors: Gil Mor MD, PhD Department of Obstetrics, Gynecology and Reproductive Science Yale University School of Medicine and Ayesha Alvero MD Department of Obstetrics, Gynecology and Reproductive Science Yale University School of Medicine Table of Contents: Chapter 1 (review) Modulation of Apoptosis to Reverse Chemoresistance Gil Mor, Michele K. Montagna and Ayesha B. Alvero Chapter 2. Caspase-3 Activation is a Critical Determinant of Genotoxic Stress-Induced Apoptosis Suparna Mazumder1, Dragos Plesca1,3, and Alexandru Almasan1,2, Chapter 3. Flow cytometry enumeration of apoptotic cancer cells by apoptotic rate *David Diaz, BSc *Alfredo Prieto BSc, PhD, *, Eduardo Reyes, BSc, PhD, *Hugo Barcenilla, BSc, *Jorge Monserrat, BSc, PhD, *, Melchor Alvarez-Mon, MD, PhD. Chapter 4 Detection of cancer-related proteins in fresh-frozen ovarian cancer samples using Laser capture microdissection Dan-Arin Silasi, Ayesha B. Alvero, Jechiel Mor, Rui Chen, Han-Hsuan Fu, Michele K. Montagna and Gil Mor Chapter 5 Flow cytometric detection of activated caspase-3 Richard Fox1 and Martine Aubert2 Chapter 6 The ChemoFx® Assay: An Ex Vivo Chemo-Sensitivity and Resistance Assay for Predicting Patient Response to Cancer Chemotherapy Stacey L. Brower, Ph.D., Jeffrey E. Fensterer, Jason E. Bush, Ph.D., MBA Chapter 7 Correlation of caspase activity and in vitro chemo-response in epithelial ovarian cancer cell lines Ayesha B. Alvero, Michele K. Montagna, and Gil Mor Chapter 8 Assessing Expression of Apoptotic Markers Using Large Cohort Tissue Microarrays Elah Pick, Mary M. McCarthy and Harriet M. Kluger Chapter 9 Mitochondria potential, Bax 'activation' and programmed cell death. C. Michael Knudson and Nicholas M. Brown Chapter 10 In Vitro and In Vivo Apoptosis Detection Using Membrane Permeant, Fluorescent Labeled Inhibitors of Caspases Brian W. Lee*, Michael R. Olin, Gary L. Johnson, and Robert J. Griffin Chapter 11 Caspase Activity Assays Andrew L. Niles, Richard A. Moravec, Terry L. Riss Chapter 12 Multiplex Caspase Activity and Cytotoxicity Assays Andrew L. Niles, Richard A. Moravec, Terry L. Riss Chapter 13 Homogeneous, Bioluminescent Proteasome Assays Martha A. O'Brien, Richard A. Moravec, Terry Riss, and Robert F. Bulleit Chapter 14 A simple method for profiling miRNA expression Jia-Wang Wang, PhD*, Jin Q. Cheng, Ph.D., M.D*. Chapter 15 Apoptotic Caspase Activation and Activity Jean-Berard Denault and Guy S. Salvesen Chapter 16 Biochemical Analysis of the native TRAIL Death-inducing Signalling Complex (DISC) Henning Walczak and Tobias L. Haas Chapter 17 Laser Microdissection Sample Preparation for RNA Analyses Christopher J. Vega

Blurb

Our understanding of the apoptotic pathway and its regulation has improved significantly in the last 10 years. However, as our knowledge of the intricate intracellular events increase, we realize the complexity of the regulatory mechanisms that control it. As such, Apoptosis and Cancer describes the performance of contemporary techniques for studying the biology of apoptosis and its role in cancer. This book is a collaboration between academics- and industry-based scientists. The chapters have the technical development characteristic of an academic environment and the standardized system necessary for translating a product to general use, as provided by the industry. Thus, the protocols described within this groundbreaking new volume will aid both an academic laboratory interested in further characterizing the mechanism of apoptosis and an industry laboratory interested on identifying new target molecules or screening for new compounds with potential clinical use. The authors – all leaders in the field – have created a comprehensive text that covers the newest available techniques as well as conventional basic techniques. Apoptosis and Cancer will undoubtedly provide the necessary resources to everyone interested in the area of apoptosis.