Microfluidic Techniques: Reviews and Protocols

ISBN-13: 9781617376139 / Angielski / Miękka / 2010 / 248 str.

Shelley D. Minteer

Opis

Hands-on researchers review the principles behind successful miniaturization and describe the key techniques for miniaturizing large-scale biochemical and bioanalytical methods for microchip analysis. The authors cover not only the most popular methods for the fabrication of microchips (photolithography, laser ablation, and soft lithography), but also microfluidic techniques for such bioanalytical assays and bioprocesses as DNA analysis, PCR, immunoassays, and cell reactors. Highlights include PCR on a microchip, microscale cell culturing, and the study of cellular processes on a microchip. The protocols offer step-by-step laboratory instructions, an introduction outlining the principles behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls.

Spis treści

Overview of Advances in Microfluidics and Microfabrication Shelley D. Minteer and Christine M. Moore Part I. Microfabrication Methods Introduction to Microfabrication Techniques Rabih Zaouk, Benjamin Y. Park, and Marc J. Madou Fabrication of Polydimethylsiloxane Microfluidics Using SU-8 Molds Rabih Zaouk, Benjamin Y. Park, and Marc J. Madou Fabrication of Microelectrodes Using the Lift-Off Technique Benjamin Y. Park, Rabih Zaouk, and Marc J. Madou Laser Ablation as a Fabrication Technique for Microfluidic Devices Emanuel A. Waddell Reversible, Room Temperature Bonding of Glass Devices for Microfluidics Loranelle L. Lockyear Part II. Biological Applications Solid-Phase Extraction in Packed Beds on Glass Microdevices Loranelle L. Lockyear Fabrication of Micromachined Magnetic Particle Separators for Bioseparation in Microfluidic Systems Jin-Woo Choi Hydrogel-Immobilized Antibodies for Microfluidic Immunoassays: Hydrogel Immunoassay Gloria Thomas, Emad M. El-Giar, Laurie E. Locascio, and Michael J. Tarlov Polymerase Chain Reaction in Miniaturized Systems: Big Progress in Little Devices Kalee D. Spitzack and Victor M. Ugaz Polymerase Chain Reaction on Microchips Maria C. Carles and Nikolaus J. Sucher A Sensitive Sandwich DNA Array Using Fluorescent Nanoparticle Probes Xiaojun Zhao, David T. Pierce, and Yanfu Huan Microfluidic Ethanol Biobatteries on a Microchip Shelley D. Minteer and Christine M. Moore Microfluidic Chambers for Cell Migration and Neuroscience Research Anne M. Taylor, Seog Woo Rhee, and Noo Li Jeon Microtextured Polydimethylsiloxane Substrates for Culturing Mesenchymal Stem Cells Erik T. K. Peterson and Ian Papautsky Contraction Study of a Single Cardiac Muscle Cell in a Microfluidic Chip Xiujun (James) Li and Paul C. H. Li Microscale Integrated Sperm Sorter Yaokuang Chung, Xiaoyue Zhu, Wei Gu, Gary D. Smith, and Shuichi Takayama Index

Blurb

Over the last ten years, miniaturization has dramatically shrunk the size of both cellular telephones and chemical instrumentation, and now also holds the promise of developing portable, inexpensive, and disposable analytical systems on a microchip. In Microfluidic Techniques: Reviews and Protocols, hands-on researchers review the principles behind successful miniaturization and describe the key techniques for miniaturizing large-scale biochemical and bioanalytical methods for microchip analysis. The authors cover not only the most popular methods for the fabrication of microchips (photolithography, laser ablation, and soft lithography), but also microfluidic techniques for such bioanalytical assays and bioprocesses as DNA analysis, polymerase chain reaction (PCR), immunoassays, and cell reactors. Highlights include PCR on a microchip, microscale cell culturing, and the study of cellular processes on a microchip. The protocols follow the successful Methods in Molecular Biology™ series format, each offering step-by-step laboratory instructions, an introduction outlining the principles behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls.
Comprehensive and informative, Microfluidic Techniques: Reviews and Protocols synthesizes the chemistry, biology, and engineering underlying microfluidic devices so that molecular biologists, as well as analytical and clinical chemists, can advance easily from macroscale to microscale biochemical and bioanalytical techniques.