Cancer Genomics and Proteomics: Methods and Protocols

ISBN-13: 9781617376054 / Angielski / Miękka / 2010 / 358 str.

Paul B. Fisher

Opis

Cancer Genomics and Proteomics provides a compendium of techniques and applications in gene identification and function. The approaches described in detail are state-of-the art and can be tailored to individual ongoing or planned research projects. This volume is a valuable laboratory resource for designing experiments to identify and analyze genes that are relevant to complex biological phenomena.

Recenzje

From the reviews:

"A compendium of techniques and applications that will be of wide utility to researchers interested in gene identification and function. ... this volume will be of interest to a broad audience of molecular biologists, geneticists and biochemists and will provide a valuable resource in the laboratory due to its applicability to a wide breadth of projects. ... for labs with a strong interest in proteomics and genomics it would certainly be well worth buying a copy to share. ... I would certainly recommend this book." (Barbara Guinn, Immunology News, May, 2008)

Spis treści

Table of Contents Cancer Genomics and Proteomics: Methods and Protocols, Paul B. Fisher, Ed. 1. Devanand Sarkar, Dong-chul Kang and Paul B. Fisher Reciprocal Subtraction Differential RNA Display (RSDD): An Efficient Technology for Cloning Differentially Expressed Genes 2. Habib Boukerche, Zao-zhong Su, Dong-chul Kang & Paul B. Fisher Cloning Differentially Expressed Genes Using Rapid Subtraction Hybridization (RaSH) 3. Ken C.N. Chang, Barry Komm, Nichole Boyer Arnold, and Murray Korc The Application of Differential Display as a Gene Profiling Tool 4. Fred van Ruissen and Frank Baas Serial Analysis of Gene Expression (SAGE) 5. Katia Basso, and Riccardo Dalla-Favera Gene expression profile analysis of tumors 6. Zhong Chen, Tin-Lap Lee, Xin-Ping Yang, Gang Dong, Amy Loercher, and Carter Van Waes cDNA Microarray and Bioinformatic Analysis of Nuclear Factor-KappaB Related Genes in Squamous Cell Carcinoma 7. Yan Ma, Qing Feng, Ian Pitha-Rowe, Sutisak Kitareewan and Ethan Dmitrovsky Gene Profiling Uncovers Retinoid Target Genes 8. Dong-chul Kang and Paul B. Fisher Complete Open Reading Frame (C-ORF) Technique: A Rapid and Efficient Method for Obtaining Complete Protein Coding Sequences 9. Piyali Dasgupta and Srikumar P. Chellappan Chromatin Immunoprecipitation Assays: Molecular Analysis of Chromatin Modification and Gene Regulation 10. Stephanie F. Phelps, Sharon Illenye and Nicholas H. Heintz Manipulating Genes and Gene Copy Number by BAC Transfection 11. Pulivarthi H. Rao, Subhadra V. Nandula, and Vundavalli V. Murty Molecular Cytogenetic Applications in the Analysis of Cancer Genome 12. Hetty Carraway and James Herman Evaluation of DNA Methylation 13. Renee Brissette and Neil I. Goldstein The Use of Phage Display Peptide Libraries for Basicand Translational Research 14. Shu-ichi Matzsuzawa and John C. Reed Yeast and Mammalian Two-Hybrid Systems for Studying Protein-Protein Interactions 15. Timothy E. Baroni, Michele T. Lastro, Aparna C. Ranganathan, Scott A. Tenenbaum, Douglas S. Conkin and Julio Aguirre-Ghiso Ribonomic and Short Hairpin RNA Gene Silencing Methods to Explore Functional Gene Programs Associated with Tumor Growth Arrest 16. Neil I. Goldstein and Paul B. Fisher Surface-Epitope Masking (SEM): An Immunological Subtraction Approach for Developing Monoclonal Antibodies Targeting Surface-Expressed Molecules 17. Paul Dent, Philip B. Hylemon, Steven Grant and Paul B. Fisher Approaches for Monitoring Signal Transduction Changes in Normal and Cancer Cells 18. Siddharth Balachandran and Glen N. Barber PKR in Innate Immunity, Cancer, and Viral Oncolysis 19. Hong Sun and Reshma Taneja Analysis of Transformation and Tumorigenicity using Mouse Embryonic Fibroblast Cells 20. Hong Sun, Neriman Tuba Gulbagci and Reshma Taneja- Analysis of Growth Properties and Cell Cycle Regulation using Mouse Embryonic Fibroblast Cells 21. Virginia Espina, Julia Wulfkuhle, Emanuel F. Petricoin III & Lance A. Liotta Reverse Phase Protein Microarrays for Monitoring Biologic Responses 22. Champion Deivanayagam, William J. Cook and Mark R. Walter Protein Crystallization

Blurb

Cancer Genomics and Proteomics: Methods and Protocols provides a compendium of techniques and applications that will be of profound use to researchers interested in gene identification and function. The approaches described in this volume are state-of-the-art and may be tailored to ongoing individual or planned research projects.

Reviews are written by experts in specific aspects of gene identification and full-length gene cloning, gene profiling (microarrays), chromatin modification of gene regulation, bacterial artificial chromosomes, cancer cytogenetic analyses, and gene methylation. Chapters also discuss topics such as phage display, yeast and mammalian two-hybrid systems, RNA silencing, monoclonal antibody production, kinases and signal transduction, PKR, analyses of mouse embryo fibroblasts, protein microarrays, and protein crystallization.

Cancer Genomics and Proteomics: Methods and Protocols will be of interest to molecular biologists, geneticists, cell biologists, and biochemists involved in studying genes associated with and regulating important physiological processes. This volume will serve as a valuable laboratory resource for designing experiments to identify and analyze genes that are relevant to complex biological phenomena.