Enzymes and whole cells are able to catalyze the most complex chemical processes under the most benign experimental and environmental conditions. In this way, enzymes and cells could be excellent catalysts for a much more sustainable chemical industry. However, enzymes and cells also have some limitations for nonbiological applications: fine chemistry, food chemistry, analysis, therapeutics, and so on. Enzymes and cells may be unstable, difficult to handle under nonconventional conditions, poorly selective toward synthetic substrates, and so forth. From this point of view, the transformation...
Enzymes and whole cells are able to catalyze the most complex chemical processes under the most benign experimental and environmental conditions. In t...
A comprehensive collection of robust methods for the detection of pesticide compounds or their metabolites useful in food, environmental, and biological monitoring, and in studies of exposure via food, water, air, and the skin or lungs. The readily reproducible methods range from gas and liquid chromatography coupled to mass spectrometry detection and other classic detectors, to capillary electrophoresis and immunochemical or radioimmunoassay methods. The authors have focused on extraction and cleanup procedures, in order to develop and optimize more fullyautomated and miniaturized methods,...
A comprehensive collection of robust methods for the detection of pesticide compounds or their metabolites useful in food, environmental, and biologic...
A collection of readily reproducible classic and emerging molecular methods for the laboratory isolation and identification of the pathogens, viruses, and parasites that cause food-borne disease. Among the pathogens covered are specific bacteria, including Salmonella spp, Campylobacter spp., Listeria spp., and Bacillus spp.; viruses, including noroviruses and enteroviruses; and parasites, including Cryptosporidium and seafood nematode worms. The protocols follow the successful Methods in Biotechnology series format, each offering step-by-step laboratory instructions, an introduction outlining...
A collection of readily reproducible classic and emerging molecular methods for the laboratory isolation and identification of the pathogens, viruses,...
The development of biotechnology over the last 20 years, and particularly the use of recombinant DNA techniques, has rapidly expanded the opportu- ties for human benefits from living resources. Efforts to reduce pollution, p- vent environmental damage, combat microbial infection, improve food production, and so on can each involve fermentation or the environmental - lease of microorganisms. Many products of fermentation technology, such as alcoholic beverages, bread, antibiotics, amino acids, vitamins, enzymes, and others, have been influenced by the progress of recombinant DNA techniques....
The development of biotechnology over the last 20 years, and particularly the use of recombinant DNA techniques, has rapidly expanded the opportu- tie...
Animal Cell Biotechnology: Methods and Protocols, Second Edition constitutes a comprehensive manual of state-of-the-art and new techniques for setting up mammalian cell lines for production of biopharmaceuticals, and for optimizing critical parameters for cell culture considering the whole cascade from lab to final production. The volume is divided into five parts that reflect the processes required for different stages of production. In Part I, basic techniques for establishment of production cell lines are addressed, especially transduction techniques, cells for gene therapy and antibody...
Animal Cell Biotechnology: Methods and Protocols, Second Edition constitutes a comprehensive manual of state-of-the-art and new techniques for sett...
In 1962 Clark and Lyons pioneered the concept of a biosensor. They p- posed immobilizing enzymes at electrochemical detectors to form "enzyme el- trodes" in order to expand the analyte range of ther base sensor. Smce then, the field of blosensors has greatly expanded. Some of the reasons for the expansion include both advances in signal transduction technologies and the incorporation of different biological sensing elements (Table 1). As a consequence, there are now a bewildering array of permutations of the biological sensing element and signal transducers that can be used to c- struct a...
In 1962 Clark and Lyons pioneered the concept of a biosensor. They p- posed immobilizing enzymes at electrochemical detectors to form "enzyme el- trod...
The frequency of reports concemmg the interface of biological reco- tion elements to signal transduction technologies has risen dramatically over the last decade. Because any one of a wide variety of biological recognition elements (e. g., antibodies, receptors, DNA, microorganisms, or enzymes) can theoretically be interfaced with any one of a wide variety of signal transducers (e. g., optical, electrochemical, thermal, or acoustic), the potential range of devices and techniques can be bewildering. The purpose of this volume and the previous volume in this series is to provide a basic...
The frequency of reports concemmg the interface of biological reco- tion elements to signal transduction technologies has risen dramatically over the ...
Altogether, the biochemical, technical and economic limitations on existing proka- otic and eukaryotic expression systems and the growing clinical demand for complex therapeutic proteins have created substantial interest in developing new expression systems for the production of therapeutic proteins. To that end, plants have emerged in the past decade as a suitable alternative to the current production systems, and today their potential for production of high quality, much safer and biologically active complex recombinant pharmaceutical proteins is largely documented. The chapters in this...
Altogether, the biochemical, technical and economic limitations on existing proka- otic and eukaryotic expression systems and the growing clinical dem...
Phytoremediation: Methods and Reviews presents the most innovative recent methodological developments in phytoremediation research, and outlines a variety of the contexts in which phytoremediation has begun to be applied. A significant portion of this volume is devoted to groundbreaking methods for the production of plants that are able to degrade, take up, or tolerate the effects of pollutants. Phytoremediation: Methods and Reviews adopts a multidisciplinary approach to the examination of principles and practices of phytoremediation, from molecular manipulation to field...
Phytoremediation: Methods and Reviews presents the most innovative recent methodological developments in phytoremediation research, and outlines a ...
Understanding the physical and genetic structure of cereal genomes and how defined coding and non-coding regions interact with the environment to determine a phenotype are key to the future of plant breeding and agriculture. The production and characteri- tion of transgenic plants is a powerful reverse genetic strategy increasingly used in cereals research to ascribe function to defined DNA sequences. However, the techniques and resources required to conduct these investigations have, until recently, been difficult to achieve or totally lacking in wheat, barley and oat. This book brings...
Understanding the physical and genetic structure of cereal genomes and how defined coding and non-coding regions interact with the environment to dete...
