Pesticide Protocols

ISBN-13: 9781588294104 / Angielski / Twarda / 2005 / 504 str.

Jose L. Martinez Vidal; Jos L. Martnez Vidal

Opis

A comprehensive collection of robust methods for the detection of pesticide compounds or their metabolites useful in food, environmental, and biological monitoring, and in studies of exposure via food, water, air, and the skin or lungs. The readily reproducible methods range from gas and liquid chromatography coupled to mass spectrometry detection and other classic detectors, to capillary electrophoresis and immunochemical or radioimmunoassay methods. The authors have focused on extraction and cleanup procedures, in order to develop and optimize more fullyautomated and miniaturized methods, including solid-phase extraction, solid-phase microextraction, microwave-assisted extraction, and on-line tandem liquid chromatography (LC/LC) trace enrichment, among others. The protocols offer step-by-step laboratory instructions, an introduction outlining the principles behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls.

Spis treści

Part I. Analytical Methodologies to Determine Pesticides and Metabolites in Human Fat Tissues and Body Fluids Analysis of Endocrine Disruptor Pesticides in Adipose Tissue Using Gas Chromatography-Tandem Mass Spectrometry: Assessment of the Uncertainty of the Method José L. Martínez Vidal, Antonia Garrido Frenich, Francisco J. Egea González, and Francisco J. Arrebola Liébanas Determination of Pyrethroids in Blood Plasma and Pyrethroid/Pyrethrin Metabolites in Urine by Gas Chromatography-Mass Spectrometry and High-Resolution GC-MS Gabriele Leng and Wolfgang Gries A Multianalyte Method for the Quantification of Current-Use Pesticides in Human Serum or Plasma Using Isotope Dilution Gas Chromatography-High-Resolution Mass Spectrometry Dana B. Barr, Roberto Bravo, John R. Barr, and Larry L. Needham Application of Solid-Phase Disk Extraction Combined With Gas Chromatographic Techniques for Determination of Organochlorine Pesticides in Human Body Fluids Adrian Covaci A Comprehensive Approach for Biological Monitoring of Pesticides in Urine Using HPLC-MS/MS and GC-MS/MS Dana B. Barr, Anders O. Olsson, Roberto Bravo, and Larry L. Needham Urinary Ethylenethiourea as a Biomarker of Exposure to Ethylenebisdithiocarbamates Silvia Fustinoni, Laura Campo, Sarah Birindelli, and Claudio Colosio Analysis of 2,4-Dichlorophenoxyacetic Acid and 2-Methyl-4- Chloro-Phenoxyacetic Acid in Human Urine Cristina Aprea, Gianfranco Sciarra, Nanda Bozzi, and Liana Lunghini Determination of Herbicides in Human Urine by Liquid Chromatography-Mass Spectrometry With Electrospray Ionization Isabel C. S. F. Jardim, Joseane M. Pozzebon, and Sonia C. N. Queiroz Analysis of Pentachlorophenol and Other Chlorinated Phenols in Biological Samples by Gas Chromatography or Liquid Chromatography-Mass Spectrometry Ji Y. Zhang Analysis of2,4-Dichlorophenoxyacetic Acid in Body Fluids of Exposed Subjects Using Radioimmunoassay Dietmar Knopp A High-Throughput Screening Immunochemical Protocol for Biological Exposure Assessment of Chlorophenols in Urine Samples Mikaela Nichkova and M.-Pilar Marco Part II. Assessment of Inhalatory and Potential Dermal Exposure Assessment of Postapplication Exposure to Pesticides in Agriculture Joop J. van Hemmen, Katinka E. van der Jagt, and Derk H. Brouwer Field Study Methods for the Determination of Bystander Exposure to Pesticides C. Richard Glass Determination of Household Insecticides in Indoor Air by Gas Chromatography-Mass Spectrometry Edith Berger-Preiss and Lutz Elflein Assessment of Dermal and Inhalatory Exposure of Agricultural Workers to Malathion Using Gas Chromatography-Tandem Mass Spectrometry Francisco J. Egea González, Francisco J. Arrebola Liébanas, and A. Marín Pesticides in Human Fat and Serum Samples vs Total Effective Xenoestrogen Burden Patricia Araque, Ana M. Soto, M. Fátima Olea-Serrano, Carlos Sonnenschein, and Nicolas Olea Part III. Pesticide Analysis in Food Quality Criteria in Pesticide Analysis Antonia Garrido Frenich, José L. Martínez Vidal, Francisco J. Egea González, and Francisco J. Arrebola Liébanas Immunoassay Methods for Measuring Atrazine and 3,5,6-Trichloro-2-Pyridinol in Foods Jeanette M. Van Emon and Jane C. Chuang Quick, Easy, Cheap, Effective, Rugged, and Safe Approach for Determining Pesticide Residues Steven J. Lehotay Determination of Organophosphorus Pesticide Residues in Vegetable Oils by Single-Step Multicartridge Extraction and Cleanup and by Gas Chromatography With Flame Photometric Detector Alfonso Di Muccio, Anna M. Cicero, Antonella Ausili, and Stefano Di Muccio Multiclass Pesticide Analysis in Vegetables Using

Blurb

Recent advances in the analysis of pesticide residues in both environmental and food commodities hold out the possibility of analyzing many pesticides in one analytical run. In Pesticide Protocols, expert researchers-who have each perfected the techniques they write about-describe in step-by-step detail robust methods for the detection of pesticide compounds or their metabolites, techniques that are highly useful in food, environmental, and biological monitoring, and in studies of exposure via food, water, air, and the skin or lungs. The methods range from gas and liquid chromatography coupled to mass spectrometry detection and other classic detectors, to capillary electrophoresis and immunochemical or radioimmunoassay techniques. The authors apply these broad-ranging techniques to the analysis of several families of pesticides, to pesticide residues in vegetables, to pesticides in water and air, and to pesticide exposure. The authors have focused on extraction and cleanup procedures in order to develop and optimize more fully automated and miniaturized methods, including solid-phase extraction, solid-phase microextraction, microwave-assisted extraction, and on-line tandem liquid chromatography trace enrichment, among others. The protocols follow the successful Methods in Biotechnology™ series format, each offering step-by-step laboratory instructions, an introduction outlining the principles behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls.
Comprehensive and versatile, Pesticide Protocols offers analysts powerful and highly practical tools for analyzing the presence of pesticides in complex matrices.