Enzyme and Microbial Biosensors: Techniques and Protocols

ISBN-13: 9780896034105 / Angielski / Twarda / 1998 / 264 str.

Ashok Mulchandani; Kim Rogers

Opis

In 1962 Clark and Lyons pioneered the concept of a biosensor. They p- posed immobilizing enzymes at electrochemical detectors to form "enzyme el- trodes" in order to expand the analyte range of ther base sensor. Smce then, the field of blosensors has greatly expanded. Some of the reasons for the expansion include both advances in signal transduction technologies and the incorporation of different biological sensing elements (Table 1). As a consequence, there are now a bewildering array of permutations of the biological sensing element and signal transducers that can be used to c- struct a biosensor. The purpose of the two volumes of Protocols and Te- niques in Biosensors is to provide a basic reference tool and starting point for use by graduate students, postdoctoral and senior researchers, and technicians m academics, industry, and government research establishments, to enable rapid entry into the field of biosensors. There are a variety of approaches that researchers employ to select a combination of bioaffinity elements and signal transducers. One commonly used approach is to identtfy the compound or compounds of interest; identify the biological molecule that yields an appropriate recognitionlselectivtty and dynamic concentration range for the assay; and choose an assay format and signal transduction technology that will meet the analytical requirements for the proposed application, This volume, Enzyme and Microbial Biosensors: Techniques and Protocols, describes a variety of transduction technologies that have been interfaced to enzymes and microorganisms.

Recenzje

"Mulchandani and Rogers have assembled an international group of authors that presents a comprehensive panel of biosensors. . .the consistent format among chapters is useful and refreshing. . .chapters include concise descriptions of the fundamentals and developmental history of the highlighted transducer. . .every chapter includes tips for ensuring experimental success."-Molecular Biotechnology

Spis treści

Part I.Enzyme Biosensors. Principles of Enzyme Biosensors, Ashok Mulchandani. Enzyme Biosensors Based on pH Electrode Canh Tran-Minh . Enzyme Biosensors Based on Gas Electrodes, Marco Mascini and Gianna Marrazza. Enzyme Biosensors Based on ISFETs, Roland Ulber and Thomas Scheper. Enzyme Biosensors Based on Oxygen Detection, F. W. Scheller, D. Pfeiffer, F. Lisdat, C. Bauer, and N. Gajovic. Enzyme Biosensors Based on the Hydrogen Peroxide Electrode, John Woodward. Enzyme Biosensors Based on Mediator-Modified Carbon Paste Electrode, Prem C. Pandey. Enzyme Biosensors Based on Electron Transfer Between Electrode and Immobilized Peroxidases, Lo Gorton, Elisabeth Csöregi, Tautgirdas Ruzgas, Irina Gazaryan, and György Marko-Varga. Enzyme Biosensors Based on Redox Polymers, Latha Shankar, Michael G. Garguilo, and Adrian C. Michael . Enzyme Biosensors Based on Metallized Carbon Electrodes, Joseph Wang. Enzyme Biosensors Based on Conducting Polymers, Wolfgang Schuhmann. Enzyme Sensors Based on Conductimetric Measurement, Norman F. Sheppard, Jr. and Anthony Guiseppi-Elie. Enzyme Biosensors Based on Thermal Transducer/Thermistor, Kumaran Ramanathan, Masoud Khayyami, and Bengt Danielsson. Enzyme Biosensors Based on Fluorometric Detection, Ashutosh Sharma. Part II. Microbial Biosensors. Microbial Biosensors Based on Oxygen Electrodes,Klaus Riedel . Microbial Biosensors Based on Respiratory Inhibition, Yoshiko Arikawa, Kazunori Ikebukuro, and Isao Karube. Microbial BiosensorsBased on Potentiometric Detection Aleksandr L. Simonian, Evgenia I. Rainina, and James R. Wild. Microbial Biosensors Based on Optical Detection, Udayakumar Matrubutham and Gary S. Sayler. Index .

Blurb

In Enzyme and Microbial Biosensors: Protocols and Techniques, master experimentalists detail cutting-edge methods developed in their own laboratories for the construction and evaluation of enzyme- and cell-based biosensors. The enzyme biosensors detailed here are based on potentiometric, amperometric, conductimetric, optical, and thermal transducers, whereas the microbial biosensors are based on oxygen-gas electrode and optical techniques in which microorganisms are coupled to the signal transducer. All the techniques are thoroughly tested and are presented in sufficient detail to ensure robust and easily reproducible results. A companion volume, Affinity Biosensors: Protocols and Techniques, by Rogers & Mulchandani, concentrates on affinity biosensors based on optical, electrochemical, thermal, acoustic, and plasmon resource techniques as applied to nucleic acids, liposomes, and eukaryotic cells.

Clearly the optimal starting point for all graduate students, postdoctoral and senior researchers, and technicians in academia, industry, and research establishments seeking rapid entry into the field of biosensors, Enzyme and Microbial Biosensors: Protocols and Techniques instantly becomes today's leading reference. Its step-by-step descriptions of various enzyme- and microbial-based biosensor techniques will allow both novices and experienced investigators alike successfully to apply these powerful new tools, and thus sharply enhance their laboratories' productivity.