Mass Spectrometry in the Biological Sciences: A Tutorial

ISBN-13: 9780792315391 / Angielski / Twarda / 1991 / 461 str.

Michael L. Gros; Michael L. Gross; M. L. Gross

Opis

This book covers both the basic principles of contemporary mass spectrometry instrumentation as well as its application to problems in structural biochemistry. Its tutorial form serves as an introduction to those entering the field, and for established scientists who wish to apply mass spectrometry to structural problems. The expertly written chapters introduce and summarize the capabilities of sector, tandem sector, hybrid, time-of-flight, triple quadrupole, ion trap, and Fourier transform mass spectrometers. New methods, such as electrospray ionization, laser desorption, and high performance liquid chromatography are also covered. Additional chapters report case studies and approaches to the determination of peptides, nucleosides, nucleotides, and lipids.

Recenzje

`I therefore can be highly recommended to all chemists and physicist who are interested to learn about the current applicability of mass spectrometry to biological sciences and in particular to those who are practising this area of research.'
Recueil dex Travaux Chimiques des Pays-Bas
'In conclusion, Mass Spectrometry in the sciences: A Tutorial achieves its objective as a tutorial for biological mass spectrometry. This book would be an excellent complement to an introductory mass spectrometry book because it contains the most recent techniques...covered by most introductory texts....I recommend this book as an ideal primer to understanding the current status of biological mass spectrometry' 'Applied Spectroscopy' 46:10 1992

Spis treści

I: Instrumentation for Mass Analysis and Detection.- 1. Sectors and Tandem Sectors.- 2. Hybrid Tandem Mass Spectrometry.- 3. Triple Quadrupole Mass Spectrometry.- 4. Quadrupole Ion Trap Mass Spectrometers for Studies of Biomolecules.- 5. Fourier Transform Mass Spectrometry: Features, Principles, Capabilities, and Limitations.- 6. Time-of-Flight Mass Spectrometers.- 7. Ion Detection in Mass Spectrometry.- II: Methods in Mass Spectrometry.- 8. Electrospray Mass Spectrometry.- 9. Laser Desorption Mass Spectrometry. Part I: Basic Mechanisms and Techniques.- 10. Laser Desorption Mass Spectrometry. Part II: Performance and Applications of Matrix-Assisted Laser Desorption/Ionization of Large Biomolecules.- 11. Laser Desorption, Chemical Ionization, and Laser Desorption/Chemical Ionization Applications with Fourier Transform Mass Spectrometry.- 12. Plasma Desorption Mass Spectrometry: Principles and Applications to Protein Studies.- 13. Continuous Flow Fast Atom Bombardment Mass Spectrometry in Analytical Biochemistry.- 14. Combined Liquid Chromatography/Mass Spectrometry (LC/MS): A Review.- 15. Combined Supercritical Fluid Chromatography/Mass Spectrometry (SFC/MS).- 16. Determination of Biomolecules by Using Liquid Chromatography and Thermospray Mass Spectrometry: from Penicillins to Peptides.- 17. Neutralization-Reionization Mass Spectrometry: Fundamental Studies and Analytical Applications.- 18. Some Applications of the Quantum-Mechanical Semiempirical Methods to the Gas-Phase Chemistry of Bio-Organic Ions.- III: Applications to Biomolecules.- 19. Characterization of Human Hemoglobin Variants by Mass Spectrometry.- 20. Detection of Post-Translational Modification of Proteins by Mass Spectrometry.- 21. Manipulation of Experimental Variables for FAB MS and MS/MS to Facilitate Identification of a Post-Translational Modification in Rabbit Elongation Factor l?.- 22. Use of FAB MS and MS/MS in the Structural Determinations of Cockroach Neuropeptides.- 23. The Mass Spectrometric Determination of Retro-Inverso Linear Peptides.- 24. Mass Spectrometry in Studies of O-Linked Glycopeptides Isolated from Natural and Recombinant Glycoproteins.- 25. Nucleosides and Oligonucleotides: Structures and Principles of Reactivity from Mass Spectrometry.- 26. Application of Tandem Mass Spectrometry to Carcinogen-Modified Nucleosides.- 27. Structure Determination of Lipids: Comparison of Classical Methods and New Approaches Involving Charge-Remote Fragmentation.- 28. The Use of FAB-MS of Cellular Lipids for the Characterization of Medically Important Bacteria.- 29. Structural Analysis of Phosphatidylinositol from Carrot Cell Membranes by Fast Atom Bombardment and Tandem Mass Spectrometry.