Kategoria BISAC: Science >> Microscopes & Microscopy

Detecting Signals at the Single Molecule Level: Pioneering Achievements in Microscopy

Recent advances have led to such remarkable improvements in fluorescence lifetime imaging microscopy's (FLIM) capacity for contrast and sensitivity that researchers can now employ it to detect signals at the single molecule level. FLIM also offers the additional benefit of independence from fluorophore concentration and excitation intensity. Moreover, its unique sensitivity makes it an excellent reporter of conformational changes and of variations in the molecular...

"Alberto Diaspro has been choreographing light's dance for over 20 years,
and in Nanoscopy and Multidimensional Optical Fluorescence Microscopy, he has assembled a diverse group of experts to explain the methods they use to coax light to reveal biology's secrets."
- From the Foreword by Daniel Evanko, editor, Nature Methods

Nanoscopy and Multidimensional Optical Fluorescence Microscopy demonstrates that the boundaries between sciences do blur at the bottom, especially those that might separate the optical work of physicists...

In the seventeenth century the microscope opened up a new world of observation, and, according to Catherine Wilson, profoundly revised the thinking of scientists and philosophers alike. The interior of nature, once closed off to both sympathetic intuition and direct perception, was now accessible with the help of optical instruments. The microscope led to a conception of science as an objective, procedure-driven mode of inquiry and renewed interest in atomism and mechanism. Focusing on the earliest forays into microscopical research, from 1620 to 1720, this book provides us with both a...

As part of the Reliable Lab Solutions series, Techniques in Confocal Microscopy brings together chapters from volumes 302, 307 and 356 of Methods in Enzymology. It documents many diverse uses for confocal microscopy in disciplines that broadly span biology.

• Documents many diverse uses for confocal microscopy in disciplines that broadly span biology
• The methods presented include shortcuts and conveniences not included in the initial publications
• Techniques are described in a context that allows comparisons to other related...

"Supplementary material for this book can be found by entering ISBN 9780470638828 on...

Molecular recognition or biorecognition is the heart of all biological interactions. Originating from protein stretching experiments, dynamical force spectroscopy allows for the extraction of detailed information on the unbinding process of biomolecular complexes. It is becoming progressively more important in biochemical studies and is finding wider applications in areas such as biophysics and polymer science. This book covers the most recent ideas and advances in the field of DFS applied to biorecognition. Leading experts in the field offer their perspectives on such important topics as...