Light Microscopy: Methods and Protocols

ISBN-13: 9781607619499 / Angielski / Twarda / 2010 / 244 str.

Helio Chiarini-Garcia; Rossana C. N. Melo

Opis

This book explores the basics and the latest advances in microscope instrumentation, sample preparation, and imaging techniques. Each chapter offers step-by-step methods, lists of necessary materials, and tips on troubleshooting and avoiding known pitfalls.

Recenzje

From the reviews:

"The book is superbly illustrated with the great majority of the figures in colours and is certainly a must not only for beginners ... but I am pretty sure that even the practised colleagues in doing every day use of microscopic methods will find very useful tricks ... . thanks to this well planned and clearly written book we are stimulated to imagine new applications." (Carlo Alberto Redi, European Journal of Histochemistry, Vol. 55, 2011)

Spis treści

TABLE OF CONTENTS: Table of Contents Preface Contributors Part I. Bright-field Microscopy Applications 1. Glycol methacrylate embedding for improved morphological, morphometrical and immunohistochemical investigations under light microscopy: testes as a model Hélio Chiarini-Garcia, Gleydes Gambogi Parreira and Fernanda Raddichi Almeida 2. Histological processing of teeth and periodontal tissues for light microscopy analysis. Gerluza A. Borges Silva, Adriana Moreira and José Bento Alves 3. Large plant samples: how to process for 2-hydroxyethyl methacrylate embedding? Élder Antônio Sousa Paiva, Sheila Zambello de Pinho and Denise Maria Trombert Oliveira 4. Image cytometry: nuclear and chromosomal DNA quantification Carlos Roberto Carvalho, Wellington Ronildo Clarindo and Isabella Santiago de Abreu 5. Histological approaches to study tissue parasitism during Trypanosoma cruzi infection Daniela L. Fabrino, Lívia Teixeira, Grazielle Ribeiro and Rossana C.N. Melo 6. Intravital microscopy to study leukocyte recruitment in vivo Vanessa Pinho, Fernanda Matos Coelho, Gustavo Batista Menezes and Denise Carmona Cara Part II. Fluorescence Microscopy Applications 7. Introduction to fluorescence microscopy Ionita Ghiran 8. Using the fluorescent styryl dye FM1-43 to visualize synaptic vesicles exocytosis and endocytosis in motor nerve terminals Ernani Amaral, Silvia Guatimosim and Cristina Guatimosim 9. Imaging lipid bodies within leukocytes with different light microscopy techniques Rossana C. N. Melo, Heloísa D´Ávila, Patrícia T. Bozza and Peter F. Weller 10. EicosaCell – An immunofluorescent-based assay to localize newly synthesized eicosanoid lipid mediators at intracellular sites. Christianne Bandeira-Melo, Peter F. Weller and Patricia T. Bozza 11. Nestin-driven green fluorescent protein as an imaging marker for nascent blood vessels in mouse models of cancer Robert M. Hoffman 12. Imaging calcium sparks in cardiac myocytes Silvia Guatimosim, Cristina Guatimosim and Long-Sheng Song 13. Light microscopy in aquatic ecology: methods for plankton communities studies Maria Carolina S. Soares, Lúcia M. Lobão, Luciana O. Vidal, Natália P. Noyma, Nathan O. Barros, Fábio Roland 14. Fluorescence immunohistochemistry in combination with differential interference contrast microscopy for studies of semi-ultrathin specimens of epoxy resin-embedding samples. Shin-ichi Iwasaki and Hidekazu Aoyagi

Blurb

Of all scientific instruments, probably none has had more applications in the life sciences than the light microscope. In Light Microscopy: Methods and Protocols, expert researchers explore the basics and the latest advances in microscope instrumentation, sample preparation, and imaging techniques, all of which have been producing fundamental insights into the functions of cells and tissues. Chapters cover a variety of bright field and fluorescence microscopy-based approaches that are central to the study of a range of biological questions, providing information on how to prepare cells and tissues for microscopic investigations, covering detailed staining procedures, and exploring methods to analyze images and interpret the results accurately. Composed in the highly successful Methods in Molecular Biology™ series format, each chapter contains a brief introduction, step-by-step methods, a list of necessary materials, and a Notes section which shares tips on troubleshooting and avoiding known pitfalls. Comprehensive and current, Light Microscopy: Methods and Protocols is an essential handbook for all researchers who are exploring the intriguing microscopic world of the cell.