This laboratory manual includes the latest tools and techniques involved in genomic research. It starts with an introductory chapter on genomics and the various tools and applications involved. The initial chapters present protocols for basic techniques such as DNA isolation, electrophoresis, PCR, cDNA synthesis etc. The book then goes on to describe more advanced techniques such as next-generation sequencing, exome sequencing, use of RNAi, RNAseq, genome editing, single cell genomics etc. Each topic includes a brief description, information on the principles involved, materials & methods,...

This volume provides basic insight and protocols relating to endophytic microbes. Chapter are divided into five major sections detailing basic isolation, bioactive metabolites production. endophytism, isolation and identification of endophytes, bioactive potentials, and screening of metabolites.Authoritative and cutting-edge,Endophytic Microbes: Isolation, Identification, and Bioactive Potentialsaims to provide comprehensive and accessible methods to undergraduate, graduate, and established scientist.

This volume details techniques involved to study aquatic pathogens that cause infections, especially in fish. Chapters guide readers through a wide range of basic and advanced methods, viral and fungal pathogens, probiotic bacteria, treatment of pathogens using seaweed extract, medicinal plant extracts, and actinomycetes.Authoritative and cutting-edge,Aquaculture Microbiologyaims to be a useful practical guide to researches to help further their study in this field.

This volume details techniques on the study of Isolation, characterization, and exploration of actinobacteria in industrial, food, agricultural, and environmental microbiology. Chapters cover a wide range of basic and advanced techniques associated with research on isolation, characterization and identification of actinobacteria in soil, sediment, estuarine, water, Saltpan, Mangroves, plants, lichens, sea weeds, sea grass, animals-crab, snail, shrimp.Authoritative and cutting-edge,Methods in Actinobacteriologyaims to be a useful practical guide to researches to help further their study in...

This volume focuses upon pollen cryopreservation in various crops from the most widely cultivated tomato to the lesser known medicinal species likeDecalepis arayalpatra.Chapters detail standard techniques such as pollen collection, cryopreservation, pollen , germination tests for viability, and specific crops. Step-by-step protocols are complemented by personal notes, precautions, and specifying the reagents to be used in each step to ensure the repeatability of the procedure across labs.Authoritative and cutting-edge,Pollen Cryopreservation Protocolsaims to serve as reference for researchers...

This book comprehensively covers in an easy-to-understand language, the principles and working of advanced analytical techniques used in Dairy Chemistry research. It begins with the basic laboratory techniques and progresses towards techniques like spectroscopy, membrane processes, western blotting etc. It provides step-by-step protocols and troubleshooting guides for easy reproduction. These one-of-a-kind protocols book is specifically designed for techniques used in Dairy Science research. It discusses all the necessary steps in different techniques, starting from sample preparations,...

This detailed new edition compiles fully updated practical methodologies and approaches for diagnosticians and researchers working toward a better understanding of animal coronaviruses. The book explores vital techniques in virus isolation, diagnostic approaches via the detection of viral nucleic acids or proteins, serology, disease-targeted animal species models, and next-generation sequencing and bioinformatics. Written for the invaluableSpringer Protocols Handbooksseries, chapters contain readily reproducible laboratory protocols as well as expert tips on troubleshooting and avoiding known...

This volume provides a wide range of aspects related to mycoremediation, which can be applied for both basic and advanced multidisciplinary research. Chapters guide readers through screening of fungi, Polyaromatic Hydrocarbons (PAHs), textile dyes, pesticides, bioreactors, molecular methods, redictive Mycology and Proteomics approaches to select fungi, elucidating biological mechanisms, and fungal laccase enzyme-based biosensors for the detection of environmental contaminants.Authoritative and cutting-edge,Mycoremediation Protocolsaims to be a  practical guide on the functional properties...