Endophytic Microbes: Isolation, Identification, and Bioactive Potentials

ISBN-13: 9781071628263 / Angielski / Twarda / 2022 / 272 str.

A. Sankaranarayanan; N. Amaresan; Mitesh Kumar Dwivedi

Opis

This volume provides basic insight and protocols relating to endophytic microbes. Chapter are divided into five major sections detailing basic isolation, bioactive metabolites production. endophytism, isolation and identification of endophytes, bioactive potentials, and screening of metabolites.Authoritative and cutting-edge, Endophytic Microbes: Isolation, Identification, and Bioactive Potentials aims to provide comprehensive and accessible methods to undergraduate, graduate, and established scientist.

Spis treści

Part I: Cultivation Methods of Bacterial Endophytes

1. Isolation of endophytic bacteria from leaves, stems or roots

Joyce Prisca Bakuwa Njoloma

 

2. Heat-treatment Method for Isolation of Bacillus spp. from Plant Tissues

T.R. Kavitha, C. Suneetha, and T.R. Sunitha

 

3. Isolation and detection of endophytic Streptomyces from Actinorhizal Casuarina root nodules

Thirumagal Thirugnanam and Dhanasekaran Dharumadurai

 

4. Isolation of endophytic fungi from stems, leaves or roots

Ambikapathy V., N.Sengottaian, Shijila Rani A.S., Babu S, and Anbukumaran A.

 

Part II: Biochemical Methods for Identification of Endophytes

5. Detection of endophytes by Cultivation method

PaulaRodríguez,David Gonzalez, and Sonia Rodríguez Giordano

 

6. Detection of Endophytes by Visualization Methods

Vijayalakshmi Selvakumar, Karnan Muthusamy, Thenmozhi Manivel, Preethi Gopalsamy^, Panneerselvam Annamalai, Ramachandran Chelliah, and Deog-Hwan Oh

7. Detection of endophytes by Immunological methods

Ravikumara, B.M, Manjunatha, L, Subathra, K, Narasareddy, G, Jyothi, G, and Prashantha, C

 

8. Detection of Endophytes by Electron Microscope

Pradeep Kumar, Simran Rani, Sarita, Amita Suneja Dang,and Pooja Suneja

 

9. Detection of Endophytes by Reactive Oxygen Staining

Simran Rani,Pradeep Kumar, Deepika, Amita Suneja Dang, and  Pooja Suneja

 

Part III: Molecular Methods for Identification of Endophytes

10. Detection of Endophytes by Electron Microscope

Pradeep Kumar, Simran Rani, Sarita, Amita Suneja Dang, and Pooja Suneja

 

11. Identification of fungal endophytes by ITS rDNA technique

Pooja Suneja, Pradeep Kumar,Simran Rani, Simran, and AmitaSuneja Dang

 

12. Identification of Fungal Endophytes by 28srDNATechnique

Karnan Muthusamy, Tamilkani Pichai, Vijayalakshmi Selvakumar, Anbukumaran Ananthan, Reshma devi Ramesh, Leelavathi Lakshmanan, Senthilkumar govindarajan, Panneerselvam Annamalai, and Ki Choon Choi

 

13. Detection of endophytes by molecular fingerprinting techniques

Manjunatha, L., Narasareddy, G., Ravikumara, B.M, LeelaSaisree Uppala, Rishikesh Kumar, Manjunatha, N, Keerthi, M.C., Mishra, R. K, and Bansa Singh

 

14. Metagenomics and Pyrosequencing Approach

Jean Carlos Vencioneck Dutra and Maria do Carmo Pimentel Batitucci

 

Part IV: Methods to Proof Endophytism

15. Cultivation-based studies of endophytic fungi in plants

Lizzy A. Mwamburi

 

16. Microscopy Based Studies

Brígido C and Paço A

 

17. Surrogate host test

Harshida A. Gamit and Natarajan Amaresan

 

18. Hood and Shew staining

Harshida A. Gamit and NatarajanAmaresan

 

Part V: Endophytes for Bioactive Metabolites

19. Screening of endophytes for plant growth promoting metabolites

Rinka S. Pramanik, SapnaChandwani, and NatarajanAmaresan

 

20. Screening of endophytes for biocontrol properties

Shah Rushabh, Sapna Chandwani, and Natarajan Amaresan

 

21. Screening of Endophytes for Biocatalytic Tools

David Gonzalez,Emiliana Botto, Sonia Rodríguez Giordano, and Paula Rodríguez

 

22. Screening of Endophytes for Biosurfactant Production

Ambikapathy V., Babu S., Anbukumaran A. and Shijila Rani A.S.

 

23. Screening of Endophytes for Antibacterial Properties

Veerapagu M, Jeya KR and Sankaranarayanan A

  24. Screening of endophytes for Nematicidal activities Soma Barman, Ratan Chowdhury, and Satya Sundar Bhattacharya

 

25. Screening of Endophytes for Virucidal Activity

Manjunatha, L., Narasa Reddy, G., LeelaSaisree Uppala, Ravikumara, B.M, and Ranjini, R

 

26. Screening of Endophytes for Fungicidal Activities

Jeya KR, Veerapagu M,  and Sankaranarayanan A

 

27. Screening of endophytes for anti-cancer compounds

Drielly Dayanne Monteiro dos Santos Baliza, Juliana Fonseca Moreira da Silva, Eskálath Morganna Silva Ferreira, IrlonMaciel Ferreira, José Luiz Martins do Nascimento, and Raphael Sanzio Pimenta