This detailed volume provides diagnosticians and researchers with practical methodologies and approaches to tackle animal coronaviruses. It explores conventional immunohistochemistry, virus neutralization, enzyme-linked immunosorbent assays, expression and purification of recombinant viral proteins, and various molecular assays, including conventional and real-time reverse transcription-PCR, reverse genetics methodology, and next generation sequencing and sequence analyses. As part of the Springer Protocols Handbooks series, chapters contain readily reproducible laboratory protocols...
This detailed volume provides diagnosticians and researchers with practical methodologies and approaches to tackle animal coronaviruses. It explore...
This detailed new edition compiles fully updated practical methodologies and approaches for diagnosticians and researchers working toward a better understanding of animal coronaviruses. The book explores vital techniques in virus isolation, diagnostic approaches via the detection of viral nucleic acids or proteins, serology, disease-targeted animal species models, and next-generation sequencing and bioinformatics. Written for the invaluableSpringer Protocols Handbooksseries, chapters contain readily reproducible laboratory protocols as well as expert tips on troubleshooting and avoiding known...
This detailed new edition compiles fully updated practical methodologies and approaches for diagnosticians and researchers working toward a better und...
