In recent years Molecular Biology has experienced an unprecedented revolution by the discovery of functional small RNAs. The number of cellular processes in which non-coding RNAs are involved is growing rapidly and include gene regulation on the transcriptional, post-transcriptional and translational level. To complicate matters, these processes seem to be strongly interconnected on the one hand, and diverse among different organisms on the other. This volume describes strategies for the discovery and validation of small RNAs and provides a snapshot of our current understanding of the...
In recent years Molecular Biology has experienced an unprecedented revolution by the discovery of functional small RNAs. The number of cellular proces...
Restriction enzymes are highly specific nucleases which occur ubiquitously among prokaryotic organisms, where they serve to protect bacterial cells against foreign DNA. Many different types of restriction enzymes are known, among them multi-subunit enzymes which depend on ATP or GTP hydrolysis for target site location. The best known representatives, the orthodox type II restriction endonucleases, are homodimers which recognize palindromic sequences, 4 to 8 base pairs in length, and cleave the DNA within or immediately adjacent to the recognition site. In addition to their important...
Restriction enzymes are highly specific nucleases which occur ubiquitously among prokaryotic organisms, where they serve to protect bacterial cells...
With the dramatic increase in RNA 3D structure determination in recent years, we now know that RNA molecules are highly structured. Moreover, knowledge of RNA 3D structures has proven crucial for understanding in atomic detail how they carry out their biological functions. Because of the huge number of potentially important RNA molecules in biology, many more than can be studied experimentally, we need theoretical approaches for predicting 3D structures on the basis of sequences alone. This volume provides a comprehensive overview of current progress in the field by leading practitioners...
With the dramatic increase in RNA 3D structure determination in recent years, we now know that RNA molecules are highly structured. Moreover, knowl...
Ribonucleases are a ubiquitous and functionally diverse group of enzymes that have a common ability to cleave RNA. Either through scission of internal phosphodiesters, or removal of nucleotides from RNA 5' or 3' ends, ribonucleases perform essential roles in gene expression and regulation, genome replication and maintenance, host defense, stress response, and viral strategies of infection. Ribonucleases have also served as highly informative models to understand virtually every aspect of biomolecular structure and function. The fifteen chapters in this volume are written by recognized...
Ribonucleases are a ubiquitous and functionally diverse group of enzymes that have a common ability to cleave RNA. Either through scission of inter...
Trypanosomes are unicellular protozoa of ancient evolutionary origin that are responsible for several tropical diseases, such as African sleeping sickness. Over the last few decades, research in trypanosome biology has revealed many unique and fascinating features, many of which have helped to establish new paradigms in other biological systems. This applies in particular to studies in gene expression and regulation, which benefit enormously from the trypanosome genome projects and from the new genome-wide approaches recently introduced in trypanosome research. This volume covers the most...
Trypanosomes are unicellular protozoa of ancient evolutionary origin that are responsible for several tropical diseases, such as African sleeping sick...
DNA is the central repository of genetic information in the cell, yet it is under constant attack by chemical mutagens, radiation and other processes. Cells therefore put a great deal of resources into repairing any damage to this precious store. Mechanisms of DNA repair vary greatly in their level of complexity, from specific reversal mechanisms that involve a single protein, to sequential pathways that require many enzymes. But at the heart of all these mechanisms lie proteins that recognize damage to DNA, raising important questions about how damaged DNA may be distinguished. These...
DNA is the central repository of genetic information in the cell, yet it is under constant attack by chemical mutagens, radiation and other processes....
Molecular biology is one of the most rapidly developing and at the same time most exciting disciplines. The key to molecular biology lies in the understanding of nucleic acids - their structure, function, and interaction with proteins. Nucleic Acids and Molecular Biology keeps scientists informed of the explosively growing information and complies with the great interest in this field by offering a continued high standard of review. A substantial part of this volume has been devoted to the analysis of different aspects of nucleic acid-protein-interactions including...
Molecular biology is one of the most rapidly developing and at the same time most exciting disciplines. The key to molecular biology lies in the under...
A wide range of topics are covered, including articles on nucleic acid structure, through their interactions with proteins to the control of gene expression. A number of authors address the subject of RNA, including the difficult but important subject of its chemical synthesis, the complexities of its structures and the mechanisms of transcript splicing. The probing of DNA structure is reviewed in papers on the application of hydroxyl radical and 1,10 phenanthroline copper cleavages. A number of important DNA-protein interactions are discussed, including DNA polymerase, the tryptophan and...
A wide range of topics are covered, including articles on nucleic acid structure, through their interactions with proteins to the control of gene expr...
Molecular biology is one of the most rapidly developing and at the same time most exciting disciplines. The key to molecular biology lies in the understanding of nucleic acids - their structure, function, and interaction with proteins. Nucleic Acids and Molecular Biology was created to keep scientists abreast of the explosively growing information and to comply with the great interest in this field.
Molecular biology is one of the most rapidly developing and at the same time most exciting disciplines. The key to molecular biology lies in the under...
Restriction enzymes are highly specific nucleases which occur ubiquitously among prokaryotic organisms, where they serve to protect bacterial cells against foreign DNA. Many different types of restriction enzymes are known, among them multi-subunit enzymes which depend on ATP or GTP hydrolysis for target site location. The best known representatives, the orthodox type II restriction endonucleases, are homodimers which recognize palindromic sequences, 4 to 8 base pairs in length, and cleave the DNA within or immediately adjacent to the recognition site. In addition to their important...
Restriction enzymes are highly specific nucleases which occur ubiquitously among prokaryotic organisms, where they serve to protect bacterial cells...
