Nucleic Acids and Molecular Biology

ISBN-13: 9783642837111 / Angielski / Miękka / 2012 / 272 str.

Fritz Eckstein;David M. J. Lilley

Opis

A wide range of topics are covered, including articles on nucleic acid structure, through their interactions with proteins to the control of gene expression. A number of authors address the subject of RNA, including the difficult but important subject of its chemical synthesis, the complexities of its structures and the mechanisms of transcript splicing. The probing of DNA structure is reviewed in papers on the application of hydroxyl radical and 1,10 phenanthroline copper cleavages. A number of important DNA-protein interactions are discussed, including DNA polymerase, the tryptophan and deoR repressors, and the resolvase enzymes which cleave Holliday junctions in recombination. Gene transcription is also covered, from the points of view of DNA methylation, mammalian ribosomal and avian lysozyme genes, and the control of transcription in the proto-oncogene c-fos. Finally, the plant kingdom has not been forgotten with articles on development and transposition in plants.

Spis treści

Structural Studies of DNA Through Cleavage by the Hydroxyl Radical.- DNase Activity of 1,10-Phenanthroline-Copper Ion.- Structure of E. coli DNA Polymerase I, Large Fragment, and Its Functional Implications.- Resolution of Model Holliday Junctions in Vitro.- Structure and Mechanism of the trp Repressor/Operator System.- The deoR Repressor from E. coli and Its Action in Regulation-at-a-Distance.- Complexities in Gene Regulation by Promoter Methylation.- Transcriptional Regulation of Proto-Oncogene c-fos.- The Structural and Functional Domain Organization of the Chicken Lysozyme Gene Locus.- Mammalian Ribosomal Gene Transcription.- The Chemical Synthesis of Oligo- and Poly-ribonucleotides.- RNA Structure.- Pre-mRNA Splicing in Yeast.- Trans-Splicing of RNA.- Structure and Function of Bacterial RNase P.- Transposition in Plants.- Transgenic Plants and the Study of Plant Development.