Harry Baker and Rosalind Ridley have done an admirable job in assem bling this collection of articles that describe the methodology frequently used to study a group of CNS illnesses often referred to as the "prion diseases." Research on prions and the disorders that they cause has progressed relatively rapidly over the last decade since the discovery of the prion protein (PrP) that allowed the application of modem molecular biological and genetic tools. The power of these techniques is awesome and their use in deciphering the once mysterious prion diseases has brought a wealth of new...
Harry Baker and Rosalind Ridley have done an admirable job in assem bling this collection of articles that describe the methodology frequently used to...
Herpes Simplex Virus Protocols comprises a wide range of experimental protocols that should be especially useful to new workers in herpes virology. Hopefully, it will also provide information for those with experience in the field, as well as those embarking on techniques that are new to them. Obviously the range of topics covered cannot be comprehensive, but we have tried to provide protocols dealing with those procedures that are most widely used; and we have selected expert authors accordingly. We have also tried to cover the range from the more biological in vivo maneuvers to purely...
Herpes Simplex Virus Protocols comprises a wide range of experimental protocols that should be especially useful to new workers in herpes virology. Ho...
The development of cancer is a slow process and it very often takes a number of years before a tumor becomes clinically evident. No current te- nique is sensitive and specific enough to detect tumors at their earliest stage, i. e., when the tumor is smaller than one billion cells. Consequently, the d- ease is usually diagnosed in an advanced state, very often when it is already beyond the reach of therapeutic strategies. This is the main stumbling block to the secondary prevention that would reduce cancer mortality. There is hope, however, because in the last decade we have witnessed an...
The development of cancer is a slow process and it very often takes a number of years before a tumor becomes clinically evident. No current te- nique ...
Laboratory studies in hemostasis have traditionally focused on abn- malities of platelet function or the quantitative and qualitative disorders that affect the proteins involved in blood coagulation. However, over the last 10 years there has been an explosion in our understanding of the molecular bases that underlie many of the inherited and acquired disorders of hemostasis. Many of these disorders are now routinely diagnosed and assessed by methods that involve genotypic analysis. Indeed in the late 1990s the distinction between molecular methods for research and for routine diagnosis is...
Laboratory studies in hemostasis have traditionally focused on abn- malities of platelet function or the quantitative and qualitative disorders that a...
The enormous advances in molecular biology that have been witnessed in . Not recent years have had major impacts on many areas of the biological sciences least of these has been in the field of clinical bacteriology and infectious disease . Molecular Bacteriology: Protocols and ClinicalApplications aims to provide the reader with an insight into the role that molecular methodology has to play in modern medical bacteriology. The introductory chapter ofMolecular Bacteriology: ProtocolsandCli- cal Applications offers a personal overview by a Consultant Medical Microbio- gist of the impact and...
The enormous advances in molecular biology that have been witnessed in . Not recent years have had major impacts on many areas of the biological scien...
Many physiologically important receptors and enzymes are regulated by their endogenous ligands and inhibitors. Nature chose to utilize these p- tides as chemical switches since they can be efficiently synthesized and - graded in cells, thus providing an immediate response to a fast changing environment. Such properties, so critical in cell survival, make peptides less than ideal drug candidates. Other frequently desired properties in drug m- ecules include oral bioavailability, the ability to cross membranes, including the blood-brain barrier, and sufficient stability for administration 1-2...
Many physiologically important receptors and enzymes are regulated by their endogenous ligands and inhibitors. Nature chose to utilize these p- tides ...
Advances in understanding the molecular mechanisms of disease together with the advent of recombinant DNA and other technologies have opened opportunities for a vast array of novel therapeutic biopharmaceuticals and diagnostic agents. However, even natural biomolecules present a myriad of problems that limit their potential as pharmaceutical agents. Rapid degra- tion and elimination, immunological reactions, and toxicity are often asso- ated with new biopharmaceuticals, much as with conventional agents. Targeted delivery systems have the potential to increase the efficacy of existing...
Advances in understanding the molecular mechanisms of disease together with the advent of recombinant DNA and other technologies have opened opportuni...
In recent years, molecular techniques have enhanced our ability to detect sexually transmitted infections and to conduct research to further our und- standing of sexually transmitted diseases. Molecular methods to quantitate pathogen load have also been shown to be useful for the management of HIV and other viral STDs. Existing laboratory manuals for the clinical microbi- ogy laboratory often do not include molecular methods for STDs. Sexually Transmitted Diseases: Methods and Protocols is intended to fill the need for a dedicated manual that covers all the fundamental aspects of molecular...
In recent years, molecular techniques have enhanced our ability to detect sexually transmitted infections and to conduct research to further our und- ...
The aim of Hematologic Malignancies: Methods and Techniques is to review those methods most useful for the diagnosis and subsequent mana- ment of hematologic malignancies. The scope of coverage is intentionally broad, ranging from routine procedures to highly sophisticated methods not currently offered by most clinical laboratories. The latter methods were selected especially to bring into focus recent advances in molecular biology that, since they provide us with strong tools for assessing the outcome of upcoming therapeutic modalities intent on disease eradication, are expected to impact...
The aim of Hematologic Malignancies: Methods and Techniques is to review those methods most useful for the diagnosis and subsequent mana- ment of hema...
This latest addition to the Methods in Molecular Medicine series, Anti- ral Methods and Protocols, is opportune because there is an increasing int- est in discovering compounds that are effective against both chronic and acute viral infections. A number of the methods described in the volume are unp- lished and their inclusion indicates the speed at which this field is moving. This volume is not a review but each chapter contains methods validated by the experts who have spent time in developing the protocols. The hallmark of this series is the comprehensive way in which the me- ods are...
This latest addition to the Methods in Molecular Medicine series, Anti- ral Methods and Protocols, is opportune because there is an increasing int- es...