Tumor Marker Protocols

ISBN-13: 9780896033801 / Angielski / Twarda / 1998 / 500 str.

Margaret Hanausek; Zbigniew Walaszek

Opis

The development of cancer is a slow process and it very often takes a number of years before a tumor becomes clinically evident. No current te- nique is sensitive and specific enough to detect tumors at their earliest stage, i. e., when the tumor is smaller than one billion cells. Consequently, the d- ease is usually diagnosed in an advanced state, very often when it is already beyond the reach of therapeutic strategies. This is the main stumbling block to the secondary prevention that would reduce cancer mortality. There is hope, however, because in the last decade we have witnessed an explosion of reports dealing with tumor markers. In many instances, simple, noninvasive diagnostic tests are becoming available to detect the early signs ofneoplasia. Interest in early detection of neoplasia is growing among those basic scientists, cli- cians, and health professionals who realize that progress in reducing cancer mortality is dependent to a great degree on its early detection and prevention. Some disappointment usually accompanies these discoveries, because what at first glance proves to be promising, is in many instances, applicable only to a limited number of cases. What is the cause of these failures? The answer may be that cancer is a multitude of neoplastic diseases in which endogenous and exogenous etiologic factors contribute either sim- taneously or over a lifetime to the development of disease.

Spis treści

Part I. Multiple Markers: Principles and Guidelines Integrating Multiple Clinical Tests to Increase Predictive Power Harry B. Burke Statistical Considerations in the Analysis of Tumor Markers Dennis A. Johnston Selection and Development of Biomarkers for Bladder Cancer George P. Hemstreet III, Robert E. Hurst, and Rebecca B. Bonner Clinical Application of Tissue and Serum Markers in Breast Cancer Gordon F. Schwartz and Roland Schwarting Part II. Immunodiagnosis of Cancer: Serum and Tissue Markers Serum and Tissue Biomarkers in the Prognosis and Treatment of Breast Cancer Tina J. Heiken and Rajeshwari R. Mehta The Oncofetal Protein p65 in Breast Cancer Detection Margaret Hanausek, Zbigniew Walaszek, Ute Sherman, and Jerzy T. Klijanienko Determination of Tumor Ferritin Concentration in Breast Cancer Jonathan F. Head and Robert L. Elliott Immunodiagnosis of Childhood Malignancies David M. Parham and Hallie Holt Immunohistochemical Evaluation of Biomarkers in Prostatic and Colorectal Neoplasia: Principles and Guidelines William E. Grizzle, Russell B. Myers, Upender Manne, and Sudhir Srivastava Factors Affecting Immunohistochemical Evaluation of Biomarker Expression in Neoplasia William E. Grizzle, Russell B. Myers, Upender Manne, Cecil Rob Stockard, Lualhati E. Harkins, and Sidhir Srivastava Instrumentation, Accuracy, and Quality Control Issues in Development of Quantitative Fluorescence-Image Analysis (QFIA) Rebecca B. Bonner, Rubert E. Hurst, Jian Yu Rao, and George P. Hemstreet Part III. Cytogenetic Markers Cytogenetics as a Diagnostic Aid for Childhood Hematologic Disorders: Conventional Cytogenetic Techniques, Fluorescence In Situ Hybridization, and Comparative Genomic Hybridization Susanna C. Raimondi, Susan Mathew, and Ching-Hon Pui Preparation of Metaphase Chromosomes for Cytogenetic Analysis Kevin A. Hahn and Penny K. Riggs Chromosome Staining and Banding Techniques Kevin A. Hahn and Penny K. Riggs Fluorescence In Situ Hybridization to Chromosomes Penny K. Riggs and Kevin A. Hahn Detection of Rearrangements in the bcl-2 Gene Using the Polymerase Chain Reaction Ruth W. Craig, Dorothy R. Belloni, Ernest S. Kawasaki, and Norman B. Levy Part IV. Molecular Markers Applications of Tissue Microdissection in Molecular Pathology: Principles and Guidelines Michael R. Emmert-Buck, Irina A. Lubensky, Rodrigo F. Chuaqui, Larisa V. Debelenko, Cathy D. Vocke, Maria J. Merino, Paul H. Duray, W. Marston Linehan, Lance A. Liotta, and Zhengping Zhuang EWS Gene Fusions as Diagnostic Markers in Sarcomas: Principles and Guidelines Marc Ladanyi p53 Detection in Breast Cancer Penelope L. Davis and J. Dirk Iglehart Use of the Polymerase Chain Reaction Technique to Detect the t(14;18) Translocation in Lymphoid Tissue Maryalice Stetler-Stevenson and Megan S. Lim Detection of ras Gene Mutations Using Oligonucleotide Ligation Technology Faye A. Eggerding Detection of Prostate-Cancer Cells in Blood and Bone Marrow by RT-PCR Robert L. Vessella and Eva Corey Quantitative, Competitive RT-PCR Analysis of Biomarkers in the Study of Neoplasia Richard D. Hockett Jr., Miriam D. Rogers, and Sudhir Srivastava Differential Display to Define Molecular Markers and Genes That Mediate Malignancy Edward J. Pavlik, Katherine Nelson, Suseela Srinivasan, Thomas L. Johnson, and Paul D. DePriest Part V. Miscellaneous Markers and Early Detection of Cancer Transforming Growth Factor Beta: A Plasma Tumor Marker Feng-Ming Kong, Mitchell S. Anscher, and Randy L. Jirtle Anti-HMdU Autoantibodies in Human Sera as a Biomarker of Canc

Blurb

This much-needed collection of Tumor Marker Protocols presents cutting-edge methods for the detection and discovery of new cancer markers, particularly molecular tumor
markers. Each method is fully described by a hands-on expert who uses it regularly in the laboratory and has perfected it to a high degree of ready reproducibility. The book also places special emphasis on statistical considerations in the analysis of tumor markers, recent discoveries in the field of serum and tissue marker proteins, cytogenetic markers, molecular markers, and markers for the early detection of cancer.

Tumor Marker Protocols offers many promising opportunities for transferring new technologies from the realm of basic science to that of clinical practice. The well-honed procedures detailed here will enable cancer researchers and clinical oncologists alike to realize the promise of these powerful new diagnostic strategies for the detection, therapy, and successful prevention of cancer.