Antiviral Methods and Protocols

ISBN-13: 9780896035614 / Angielski / Twarda / 1999 / 404 str.

Derek Kinchington; Raymond F. Schinazi

Opis

This latest addition to the Methods in Molecular Medicine series, Anti- ral Methods and Protocols, is opportune because there is an increasing int- est in discovering compounds that are effective against both chronic and acute viral infections. A number of the methods described in the volume are unp- lished and their inclusion indicates the speed at which this field is moving. This volume is not a review but each chapter contains methods validated by the experts who have spent time in developing the protocols. The hallmark of this series is the comprehensive way in which the me- ods are described, which includes a list of all the reagents needed for each protocol. Of importance is the section on tips and pitfalls that the authors have discovered while developing their protocols. The manual itself is designed to be used by researchers in universities and industry who are familiar with a range of biological techniques but who want to set up quickly a novel assay system. We encourage a dialog between readers and authors, which may also result in useful collaborations.

Recenzje

"...will be of great benefit to all involved with antiviral development..." -Antiviral Research

"...Together with some useful general discussions on safety and new approaches to antiviral research this volume offers extremely useful information for both the pharmaceutical industry and the academic researcher."-Microbiology Today

"...the editors are to be congratulated for assembling an impressive array of experts in the field as authors for the chapters and the book should remain a valuable reference work for many years, despite the rapidly moving nature of the discipline." - Journal of Antimicrobial Chemotherapy

Spis treści

Changing Methods for Discovering Antiviral Drugs Philip S. Jones Laboratory Safety Considerations Paul M. Feorino, John D. Williamson, and Raymond F. Schinazi Routine Quality Control Testing of Cell Cultures: Detection of Mycoplasma Alison Stacey and Glyn Stacey Part I. Hepatitis Virus Hep AD38 Assay: A High-Throughput, Cell-Based Screen for the Evaluation of Compounds Against Hepatitis B Virus Robert W. King and Stephanie K. Ladner Hepatitis B Virus Cell Culture Assays for Antiviral Activity Karen Schmidt and Brent Korba A Rapid Microtiter Assay for Duck Hepatitis Virus Reverse Transcriptase Michael J. Otto and Richard A. Whitaker Assays for Measuring Hepadnaviral Supercoiled DNA Stephen A. Locarnini and Gilda M. Civitico Assays for Hepatitis B Virus DNA- and RNA-Dependent DNA Polymerase Activities Timothy Shaw and Stephen A. Locarnini Development of a Hepatitis C Virus RNA Helicase High Throughput Assay Ann D. Kwong and Christine Risano Part II. Herpesvirus Determination of Viral Infectivity: HSV, VZV, and CMV David R. Harper Antiviral Screening Assays for Human Herpesviruses 6 and 7 Jodi B. Black and Philip E. Pellett Strategies for Evaluation of Antiviral Agents Against Epstein-Barr Virus in Culture Jung-Chung Lin Monitoring Drug Resistance for Herpesviruses Graciela Andrei, Pierre Fiten, Erik De Clercq, Robert Snoeck, and Ghislain Opdenakker Herpesvirus Protease Assays Peter Ertl, Linda Russell, and Jane Angier Part III. Human Immunodeficiency Virus Testing Compounds for Antiviral Activity in Cell Cultures Infected with HIV Donna Devine, Naomi Mathews, and Derek Kinchington Evaluation of Compounds That Prevent Reactivation of HIV-1 in OM-10.1 Cells Salvatore T. Butera Assessment of Activity of Topical Virucidal Agents Timothy O'Connor Assays That Measure Selective Killing of Virus-Infected Cells Sharon Randall Evaluating Clinical Isolates for Their Phenotypic and Genotypic Resistance Against Anti-HIV Drugs Anne-Mieke Vandamme, Myriam Witvrouw, Christophe Pannecouque, Jan Balzarini, Kristel Van Laethem, Jean-Claude Schmit, Jan Desmyter, and Erik De Clercq Line Probe Assay for Detecting Mutations in HIV-1 Reverse Transcriptase Lieven Stuyver, Ann Wyseur, Gonda Verpooten, and Rudi Rossau RT-PCR for Amplification of Specific Fragments of HIV-1 Genome Marie-Pierre de Béthune and Kurt Hertogs Evaluation of Compounds Against Recombinant HIV Reverse Transcriptase Jan Balzarini Ultrasensitive Detection of Reverse Transcriptase Activity by the Amp-RT Assay: Applications to the Measurement of Virus Loads and Phenotypic Drug Resistance J. Gerardo García Lerma and Walid Heneine Reverse Transcriptase Assay Based on Product Enhancement for Assessing the Drug Susceptibility of Retroviruses Jürg Böni and Jörg Schüpbach Identifying and Characterizing HIV Protease Inhibitors Eric S. Furfine Retroviral Integrase: A Novel Target in Antiviral Drug Development and Basic In Vitro Assays with the Purified Enzyme Abhijit Mazumder, Nouri Neamati, Sanjay Sunder, Joshua Owen, and Yves Pommier Part IV. Human Papilloma Virus Papillomavirus DNA Replication Maureen C. Ferran and Alison A. McBride Quantitative Immunodetection of Viral Oncoprotein Levels in Human Papillomavirus-Transformed Human Cells Jeffery A. Johnson and J. David Gangemi Part V. Influenza Viruses Biochemical Methods for the Characterization of Influenza Viruses with Reduced Sensitivity to 4-Guanidino-Neu5Ac2en Richard C. Bethell, Graham J. Hart, Tony J. Blick, Anjali Sahasrabudhe,

Blurb

In Antiviral Methods and Protocols, experienced scientists describe-in an easily-followed format-their state-of-the-art techniques for evaluating antiviral compounds. The assays described include systems for investigating drugs used against herpesviruses, hepatitis viruses, human immunodeficiency viruses, human papillomaviruses, and influenza viruses. These well-tested methods range from cellular assays to some of the most up-to-date molecular approaches for identifying compounds that are active against viral enzymes and the development of viral resistance against drugs currently in use. The detailed protocols also discuss possible pitfalls and ways to overcome them.
Timely and comprehensive, Antiviral Methods and Protocols offers today's researchers in academia, clinical departments, and the pharmaceutical industry the powerful, reproducible, and novel methods needed to evaluate compounds effective against both acute and chronic infections.