Molecular Bacteriology: Protocols and Clinical Applications

ISBN-13: 9780896034983 / Angielski / Twarda / 1998 / 682 str.

Neil Woodford; Alan Johnson

Opis

The enormous advances in molecular biology that have been witnessed in . Not recent years have had major impacts on many areas of the biological sciences least of these has been in the field of clinical bacteriology and infectious disease . Molecular Bacteriology: Protocols and ClinicalApplications aims to provide the reader with an insight into the role that molecular methodology has to play in modern medical bacteriology. The introductory chapter ofMolecular Bacteriology: ProtocolsandCli- cal Applications offers a personal overview by a Consultant Medical Microbio- gist of the impact and future potential offered by molecular methods. The next six chapters comprise detailed protocols for a range of such methods . We believe that the use of these protocols should allow the reader to establish the various methods described in his or her own laboratory. In selecting the methods to be included in this section, we have concentrated on those that, arguably, have greatest current relevance to reference clinical bacteriology laboratories; we have deliberately chosen not to give detailed protocols for certain methods, such as multilocus enzyme electrophoresis that, in our opinion, remain the preserve of specialist la- ratories and that are not currently suited for general use. We feel that the methods included in this section will find increasing use in diagnostic laboratories and that it is important that the concepts, advantages, and limitations of each are th- oughly understood by a wide range of workers in the field .

Recenzje

"The book is authoritative, as its many contributors are all experts in their fields; their contributions have been well coordinated by the editors, resulting in a unified work. . .The book is sturdily bound in hardback to withstand the considerable use it deserves and will undoubtedly get. It represents very good value and I recommend that all laboratories interested in molecular diagnosis in bacteriology should buy a copy of it."-Journal of Medical Microbiology

"Although intended primarily for clinical microbiologists and infectious disease specialists, this book will be an excellent reference for investigators who want to use established molecular techniques in a variety of studies. The numerous contributing authors are well regarded within their respective fields and their descriptions and interpretations of their techniques will allow readers to efficiently establish state-of-the-art methodologies in their laboratories...Each chapter contained in this volume is current and well written, and supported with an extensive and recent list of citations....This is an important reference for molecular techniques that will soon change clinical microbiology. It will be a valuable addition to the collection of libraries that serve medical and academic communities. It is an essential addition to the private libraries of clinical microbiologists and those studying infectious diseases."-Doody's Health Science Book Review Journal

". . .a book that will be useful not only to the experienced researchers, but also to the newcomer."-Laboratoriums Medizin

Spis treści

The Impact of Molecular Methods on Clinical Bacteriology R. C. George Genomic DNA Digestion and Ribotyping J. Z. Jordens Pulsed-Field Gel Electrophoresis M. E. Kaufmann Plasmid Analysis A. P. Johnson and N. Woodford DNA Amplification J. P. Clewley and N. Saunders Arbitrarily Primed PCR Methods for Studying Bacterial Diseases D. Ralph and M. McClelland Genomic Fingerprinting by Application of rep-PCR A. M. Ridley Molecular Approaches to the Identification of Streptococci R. E. McLaughlin and J. J. Ferretti Pneumococcal Diseases A. M. Smith and K. P. Klugman Tuberculosis & Other Diseases Caused by Mycobacterium spp M. Goyal and D. B. Young Diptheria A. Efstratiou, K. E. Engler, and A. de Zoysa Infection Caused by Legionella spp T. G Harrison and N.K. Fry Haemophilus Influenzae M. A. Herbert, D. Crook, and E. R. Moxon Meningococcal Infection M. C. J. Maiden Gonorrhoea C. A. Ison Chancroid S. A. Morse, D. L. Trees, and P. A. Totten Mycoplasma/Ureaplasma Infection C. B. Gilroy and D. T. Robinson Salmonella Infections E. John Threlfall, M. D. Hampton and A.M. Ridley Cholera T. J. Barrett and D. N. Cameron Diagnosis and Investigation of Diarrheagenic Escherichia coli J. P. Nataro and J. Martinez Campylobacter Infection J. Gibson and R. J. Owen Helicobacter pylori R. J. Owen and J. Gibson Staphylococci A. Deplano and M. Struelens Application of Molecular Techniques to the Study of Nosocomial Infection Caused by Enterococci T. M. Coque, P. Seetulsingh, K. V. Singh, and B. E. Murray Molecular Approaches for the Detection and Identification of ß-Lactamases D. J. Payne and C. J.Thomson Biochemical and Enzyme Kinetic Applications for the Characterization of ß-Lactamases D. J. Payne and T. H. Farmer ß-lactam Resistance Mediated by Changes in Penicillin-Binding Proteins C. G. Dowson and T. J. Coffey Application of Molecular Techniques for the Study of Aminoglycoside Resistance K. J. Shaw, F. J. Sabatelli, L. Naples, P. Mann, R. S. Hare, and G. H. Miller Molecular Investigation of Glycopeptide Resistance in Gram-Positive Bacteria N. Woodford and J. M. Stigter Quinolone Resistance J. Brown and S. G. B. Amyes Resistance to Tetracyclines, Macrolides, Trimethoprim, and Sulfonamides M. C. Roberts Index

Blurb

In Molecular Bacteriology: Protocols and Clinical Applications, Woodford and Johnson bring together in a single volume a state-of-the-art collection of detailed molecular methods for the diagnosis and clinical investigation of bacterial infections. These wide ranging techniques-each presented by recognized experts who have fine-tuned their methods for optimum performance-will permit readers to establish them successfully in their own laboratories. An accompanying series of minireviews highlights and explains the clinical and epidemiological relevance of the techniques and shows how they may be applied to major pathogen species and various types of antibiotic resistance.

Molecular Bacteriology: Protocols and Clinical Applications enlarges our current understanding of the role that molecular methodology can play in modern medical bacteriology, fully explaining both the practical and theoretical aspects of the cutting-edge methods that are increasingly finding diagnostic use in laboratories throughout the world. An authoritative laboratory resource for everyone working with infectious diseases today, this book's insightful reviews and detailed protocols will soon win many devotees.