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The genotyping results of this study suggest that type specific PCR evaluated in this study may reliably be used in genotyping studies of HCV. Both type specific PCR and serotyping has advantages and drawbacks. Serotyping assay have the advantages over molecular biology based typing methods only in terms of low cost, rapid test procedure and requirement of a few instruments that would be present in any clinical laboratory. However, ELISA is less sensitive and does not allow identification of HCV mixed infection and subtypes. Furthermore, the serotyping assay cannot differentiate between...

In the history of leishmaniasis, several
methods were used for classification, characterization and
identification of the infecting parasites. The conventional
methods of clinical diagnosis of CL have ranged from
clinical picture and epidemiological data, visualizing the
amastigotes by microscopy of stained smears from skin touch
specimens or biopsies to in-vitro culturing of the parasite.
These conventional methods are, however, limited in
sensitivity, need an experienced hand and do not...