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Today, Bioinformatics community is facing several challenges. To handle large amount of data, robust systems are required that enable bioinformatics analysis to be carried out easily. To perform fast and accurate analysis, development of new algorithms and methods is required to make sense of large quantities of data generated in large-scale efforts of biological community. This will be a valuable contribution in this context. I acknowledge my supervisor, Dr. Shahid Nadeem, Chairman, Department of Bioinformatics & Biotechnology, Government College University Faisalabad, Pakistan for his...

In the history of leishmaniasis, several
methods were used for classification, characterization and
identification of the infecting parasites. The conventional
methods of clinical diagnosis of CL have ranged from
clinical picture and epidemiological data, visualizing the
amastigotes by microscopy of stained smears from skin touch
specimens or biopsies to in-vitro culturing of the parasite.
These conventional methods are, however, limited in
sensitivity, need an experienced hand and do not...

The genotyping results of this study suggest that type specific PCR evaluated in this study may reliably be used in genotyping studies of HCV. Both type specific PCR and serotyping has advantages and drawbacks. Serotyping assay have the advantages over molecular biology based typing methods only in terms of low cost, rapid test procedure and requirement of a few instruments that would be present in any clinical laboratory. However, ELISA is less sensitive and does not allow identification of HCV mixed infection and subtypes. Furthermore, the serotyping assay cannot differentiate between...