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In the history of leishmaniasis, several
methods were used for classification, characterization and
identification of the infecting parasites. The conventional
methods of clinical diagnosis of CL have ranged from
clinical picture and epidemiological data, visualizing the
amastigotes by microscopy of stained smears from skin touch
specimens or biopsies to in-vitro culturing of the parasite.
These conventional methods are, however, limited in
sensitivity, need an experienced hand and do not...