Two-Component Signaling Systems, Part a: Volume 422

ISBN-13: 9780123738516 / Angielski / Twarda / 2007 / 551 str.

Melvin I. Simon; Brian R. Crane; Alexandrine Crane

Opis

Multicellular organisms must be able to adapt to cellular events to accommodate prevailing conditions. Sensory-response circuits operate by making use of a phosphorylation control mechanism known as the "two-component system."

Sections include:
Computational Analyses of Sequences and Sequence Alignments
Biochemical and Genetic Assays of Individual Components of Signaling Systems
Physiological Assays and Readouts

• Presents detailed protocols
• Includes troubleshooting tips

Spis treści

Section I. Computational Analyses of Sequences and Sequence Alignments

CHAPTER 1: Comparative genomic and protein sequence analyses of a complex system controlling bacterial chemotaxis.
CHAPTER 2: Two component systems in microbial communities: Approaches and resources for analyzing metagenomic data sets.
CHAPTER 3: Identification of sensory and signal-transducing domains in two-component signaling systems.
CHAPTER 4: Features of protein-protein interactions in two-component signaling deduced from genomic libraries.
CHAPTER 5: Sporulation phosphorelay proteins and their complexes: Crystallographic characterization.
CHAPTER 6: Control Analysis of Bacterial Chemotaxis Signaling.
CHAPTER 7: Classification of Response Regulators based on their Surface Properties

Section II. Biochemical and Genetic Assays of Individual Components of the Signaling Systems

CHAPTER 8: Purification and Assays of Rhodobacter capsulatus RegB-RegA Two Component Signal Transduction System.
CHAPTER 9: Purification and reconstitution of PYP-phytochrome (Ppr) with biliverdin and 4-hydroxycinnamic acid.
CHAPTER 10: Oxygen and Redox-Sensing by Two-Component Systems That Regulate Behavioral Responses. Behavioral Assays and Structural Studies of Aer using in vivo Disulfide Crosslinking.
CHAPTER 11: Two-Component Signaling in the Virulence of S. aureus: A Silkworm Larvae-Pathogenic Agent Infection Model of Virulence.
CHAPTER 12: TonB System, In vivo Assays and Characterization
CHAPTER 13: Biochemical Characterization of Plant Ethylene Receptors following Transgenic Expression in Yeast.
CHAPTER 14: Structure of SixA, a histidine protein phosphatase of the ArcB HPt domain in E. coli.
CHAPTER 15: Triggering and monitoring light-sensing reactions in protein crystals
CHAPTER 16
CHAPTER 17: Application of Fluorescence Resonance Energy Transfer to Examine EnvZ/OmpR Interactions
CHAPTER 18: Gene promoter scan (GPS) methodology for identifying and classifying co-regulated promoters.
CHAPTER 19: Targeting two-component signal transduction: A novel drug discovery system.
CHAPTER 20: The essential YycFG two-component system of Bacillus subtilis

Section III. Physiological Assays and Readouts

CHAPTER 21: Isolation and Characterization of Chemotaxis Mutants of the Lyme Disease Spirochete Borrelia burgdorferi Using Allelic Exchange Mutagenesis, Flow Cytometry and Cell Tracking.
CHAPTER 22: Phosphorylation assays of chemotaxis two-component system proteins in Borrelia burgdorferi.
CHAPTER 23: Regulation of Respiratory Genes by ResD-ResE Signal Transduction System in Bacillus subtilis.
CHAPTER 24: Physiological and genetic characterization of two-component systems in Myxococcus.
CHAPTER 25: Detection and measurement of two-component systems that control dimorphism and virulence in fungi.
CHAPTER 26: Using Two-Component Systems and other Bacterial Regulatory Factors for the Fabrication of Synthetic Genetic Devices.