From the Preface
Although it has been suspected since ancient times that metals participate in biological processes, details of the manner in which they might exert a function have until recently remained unknown. It is now appreciated that metals play roles in catalysis, hormone action, gene and other regulatory functions, structural stabilization of macromolecules, muscle contraction, nerve conduction, and transport. "Metallobiochemistry" is a field which has grown and matured rapidly over the past decade. Metalloenzymes have been the central investigating targets. This volume provides...

The critically acclaimed laboratory standard, Methods in Enzymology, is one of the most highly respected publications in the field of biochemistry. Since 1955, each volume has been eagerly awaited, frequently consulted, and praised by researchers and reviewers alike. The series contains much material still relevant today - truly an essential publication for researchers in all fields of life sciences.

The critically acclaimed laboratory standard, Methods in Enzymology, is one of the most highly respected publications in the field of biochemistry. Since 1955, each volume has been eagerly awaited, frequently consulted, and praised by researchers and reviewers alike. The series contains much material still relevant today - truly an essential publication for researchers in all fields of life sciences.

This volume, as does Volume 177, provides a general background of modern NMR techniques, with a specific focus on NMR techniques that pertain to proteins and enzymology, and a "snapshot" of the current state of the art in NMR experimental techniques. These books enable the reader to understand a given technique, to evaluate its strengths and limitations, to decide which is the best approach, and, finally, to design an experiment using the chosen technique to solve a problem.

The critically acclaimed laboratory standard, Methods in Enzymology, is one of the most highly respected publications in the field of biochemistry. Since 1955, each volume has been eagerly awaited, frequently consulted, and praised by researchers and reviewers alike. The series contains much material still relevant today - truly an essential publication for researchers in all fields of life sciences.

The methods presented in this volume will enable the reader to design effective strategies for the expression of cloned genes and cDNAs and will prove useful in solving the majority of expression problems one is likely to encounter.

This volume on Phospholipases, the first of its kind, presents techniques for the assay, purification, and characterization of these enzymes, which are not only degradative enzymes of lipid metabolism but are also key enzymes in the signal transduction pathway.

This volume provides a compilation of recent methods for studying protein phosphorylation.

This volume collates in one source methodology for in vivo genetic engineering and for genetic analysis in a wide range of bacteria. Not only is Escherichia coli well covered, but so are other emerging bacterial systems.

This book provides up-to-date, state-of-the-art methods for the purification of metallothionein and related proteins, for the study of metal binding, for the quantitation of metallothionein, and for the study of the physical and biological properties of metallothionein. New views of the structure and function of metallothionein are also included in some chapters.