Multicellular organisms must be able to adapt to cellular events to accommodate prevailing conditions. Sensory-response circuits operate by making use of a phosphorylation control mechanism known as the "two-component system." Sections include: Computational Analyses of Sequences and Sequence Alignments Biochemical and Genetic Assays of Individual Components of Signaling Systems Physiological Assays and Readouts
Presents detailed protocols
Includes troubleshooting tips
Multicellular organisms must be able to adapt to cellular events to accommodate prevailing conditions. Sensory-response circuits operate by making use...
Multicellular organisms must be able to adapt to cellular events to accommodate prevailing conditions. Sensory-response circuits operate by making use of a phosphorylation control mechanism known as the "two-component system."
Sections in Two-Component Signaling Systems, Part B include:
Structural Approaches
Reconstitution of Heterogeneous Systems
Intracellular Methods and Assays
Genome-Wide Analyses of Two-Component Systems
Presents detailed protocols
Includes troubleshooting tips
Multicellular organisms must be able to adapt to cellular events to accommodate prevailing conditions. Sensory-response circuits operate by making ...
Computer-based design and modeling, computational approaches, and instrumental methods for elucidating molecular mechanisms of protein folding and ligand-acceptor interactions are included in Volumes 202 and 203, as are genetic and chemical methods for the production of functional molecules including antibodies and antigens, enzymes, receptors, nucleic acids and polysaccharides, and drugs.
Computer-based design and modeling, computational approaches, and instrumental methods for elucidating molecular mechanisms of protein folding and lig...
This volume, as does Volume 176, provides a general background of modern NMR techniques, with a specific focus on NMR techniques that pertain to proteins and enzymology, and a "snapshot" of the current state-of-the-art in NMR experimental techniques. These books enable the reader to understand a given technique, to evaluate its strengths and limitations, to decide which is the best approach, and, finally, to design an experiment using the chosen technique to solve a problem.
This volume, as does Volume 176, provides a general background of modern NMR techniques, with a specific focus on NMR techniques that pertain to prote...
The critically acclaimed laboratory standard for more than forty years, Methods in Enzymology is one of the most highly respected publications in the field of biochemistry. Since 1955, each volume has been eagerly awaited, frequently consulted, and praised by researchers and reviewers alike. More than 260 volumes have been published (all of them still in print) and much of the material is relevant even today--truly an essential publication for researchers in all fields of life sciences.
Liquid chromatography
Electrophoresis
Mass spectrometry
The critically acclaimed laboratory standard for more than forty years, Methods in Enzymology is one of the most highly respected publications ...
The critically acclaimed laboratory standard for more than forty years, Methods in Enzymology is one of the most highly respected publications in the field of biochemistry. Since 1955, each volume has been eagerly awaited, frequently consulted, and praised by researchers and reviewers alike. More than 285 volumes have been published (all of them still in print) and much of the material is relevant even today-truly an essential publication for researchersin all fields of life sciences.
Prokaryotic ABC Transporters
Eukaryotic ABC Transporters
Nonmammalian ABC...
The critically acclaimed laboratory standard for more than forty years, Methods in Enzymology is one of the most highly respected publications ...
Multicellular organisms must be able to adapt to cellular events to accommodate prevailing conditions. Sensory-response circuits operate by making use of a phosphorylation control mechanism known as the "two-component system." This volume, the third in a three-volume treatment edited by the same group of editors, includes a wide range of methods, including those dealing with the Sln-1 kinase pathway, triazole sensitivity in C. albicans, and histidine kinases in cyanobacteria circadian clock.
Includes time-tested core methods and new innovations applicable to any...
Multicellular organisms must be able to adapt to cellular events to accommodate prevailing conditions. Sensory-response circuits operate by making ...
This volume of Methods in Enzymology covers the current methodology for the detection and assessment of constitutively active proteins. The chapters written by expert authors who are leaders in the field, provide hints and tricks not available in primary research publications.It is extensively referenced, with useful figures and tables throughout the volume.
Expert authors who are leaders in the field
Extensively referenced and useful figures and tables
Provides hints and tricks to facilitate reproduction of methods
This volume of Methods in Enzymology covers the current methodology for the detection and assessment of constitutively active proteins. The ...
This volume of Methods in Enzymology covers the current methodology for the detection and assessment of constitutively active proteins. The chapters written by expert authors who are leaders in the field, provide hints and tricks not available in primary research publications.It is extensively referenced, with useful figures and tables throughout the volume.
Expert authors who are leaders in the field
Extensively referenced and useful figures and tables
Provides hints and tricks to facilitate reproduction of methods
This volume of Methods in Enzymology covers the current methodology for the detection and assessment of constitutively active proteins. The ...
In the past several years, there has been an explosion in the ability of biologists, molecular biologists and biochemists to collect vast amounts of data on their systems. Biothermodynamics, Part C presents sophisticated methods for estimating the thermodynamic parameters of specific protein-protein, protein-DNA and small molecule interactions.
The use of thermodynamics in biological research is used as an "energy book-keeping system." While the structure and function of a molecule is important, it is equally important to know what drives the energy force. These methods look...
In the past several years, there has been an explosion in the ability of biologists, molecular biologists and biochemists to collect vast amounts o...