NMR of Proteins and Nucleic Acids

ISBN-13: 9780471828938 / Angielski / Twarda / 1991 / 320 str.

Kurt Wuthrich; Kurt W]thrich;Wuthrich

Opis

This comprehensive introduction to both underlying principles and experimental procedures, covers the structure and NMR of biopolymers, including 2D NMR and Nuclear Overhauser effects; resonance assignments and structure determination in proteins; and resonance assignments and structure determination in nucleic acids. Also provided is an account of NMR studies for chemists, biochemists, biophysicists and molecular biologists concerned with structure and function of proteins and nucleic acids. The book aims to enable specialists and non-specialists to evaluate the potentialities and limitations of the method.

Spis treści

Introduction and Survey.

THE FOUNDATIONS: STRUCTURE AND NMR OF BIOPOLYMERS.

NMR of Amino Acid Residues and Mononucleotides.

NMR Spectra of Proteins and Nucleic Acids in Solution.

The NMR Assignment Problem in Biopolymers.

Two–Dimensional NMR With Proteins and Nucleic Acids.

Nuclear Overhauser Enhancement (NOE) in Biopolymers.

RESONANCE ASSIGNMENTS AND STRUCTURE DETERMINATION IN PROTEINS.

NOE–Observable 1H–1H Distances in Proteins.

Sequence–Specific Resonance Assignments in Proteins.

Polypeptide Secondary Structures in Proteins by NMR.

Three–Dimensional Protein Structures by NMR.

RESONANCE ASSIGNMENTS AND STRUCTURE DETERMINATION IN NUCLEIC ACIDS.

NOE–Observable 1H–1H Distances in Nucleic Acids.

Resonance Assignments in Nucleic Acids Using Scalar Couplings.

Nucleic Acid Conformation, 1H–1H Overhauser Effects, and Sequence–Specific Resonance Assignments.

WITH NMR TO BIOPOLYMER CONFORMATION AND BEYOND.

Conformation of Noncrystalline Proteins and Nucleic Acids.

NMR Studies of Intermolecular Interactions with Biopolymers.

References.

Index.

O autorze

Kurt Wuthrich was a co–recipient of the 2003 Nobel Prize in Chemistry for his work on nuclear magnetic resonance technology and its application to protein analysis.