Analytical Techniques in Biochemistry and Molecular Biology

ISBN-13: 9781489996787 / Angielski / Miękka / 2014 / 441 str.

Rajan Katoch

Opis

Advances in biochemistry now allow us to control living systems in ways that were undreamt of a decade ago. This volume guides researchers and students through the full spectrum of experimental protocols used in biochemistry, plant biology and biotechnology.

Spis treści

Chapter 1: Preparation of solutions

Chapter 2: Expression of concentration

Chapter 3: Buffers and their preparation

Chapter 4: Techniques in biochemical evaluation

Chapter 5: Carbohydrate estimations

Chapter 6: Estimation of lipids

Chapter 7: Qualitative and quantitative estimations of amino acids and proteins

Chapter 8: Protein purification techniques

Chapter 9: Cell disruption and fractionation

Chapter 10: Enzymes in metabolism

Chapter 11: Isoenzyme analysis

Chapter 12: Chromatographic separations

Chapter 13: Methods for nutritional quality evaluation of food materials

Chapter 14: Nutritional evaluation of forages

Chapter 15: Techniques in molecular biology

Blurb

Long defined as a core subject in the life sciences, biochemistry now allows us to control living systems in ways undreamt of even a decade ago. Its relevance increases daily as plant biology and biotechnology move inexorably up the scientific agenda. This comprehensive volume explores the numerous experimental techniques that must be mastered by researchers in plant biology, biochemistry and biotechnology. As the interest in biochemical and molecular methods of investigating physiological processes rises, there's a concurrent development of new, faster, and more sensitive experimental procedures, allowing us to explore the inner workings of animal and plant organisms.

<i>Analytical Techniques in Biochemistry and Molecular Biology</i> guides the reader through these methodologies, beginning with the preparation of solutions and the expression of their concentrates; continuing with the concept and preparation of buffers, a key element of working with enzymes; and discussing biochemical analysis, carbohydrates, and proteins and amino acids, as well as enzyme and isozyme analysis.  This volume also examines the full spectrum of protocols used in contemporary biochemical experimentation, including chromatographic separation procedures and the nutritional evaluation of foodstuffs. Lastly, the book covers cutting-edge techniques in biotechnology. Truly multidisciplinary, <i>Analytical Techniques in Biochemistry and Molecular Biology</i> is a valuable resource to researchers in biochemistry, biotechnology and their derivative fields.