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Single-Molecule Techniques: A Laboratory Manual
ISBN: 9780879697754 / Angielski / Miękka / 516 str. Termin realizacji zamówienia: ok. 5-8 dni roboczych. As molecular and cellular biologists move toward nanotechniques for performing experiments on single molecules rather than on populations of molecules, a comprehensive manual on how (and why) to carry out such experiments is needed. Single-Molecule Techniques: A Laboratory Manual fills this requirementit is the first to take researchers who know nothing about singlemolecule analyses to the point where they can successfully design and execute appropriate experiments. Geared toward research scientists in structural and molecular biology, biochemistry, and biophysics, the manual will be useful...
As molecular and cellular biologists move toward nanotechniques for performing experiments on single molecules rather than on populations of molecules...
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cena:
197,32 |
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Retinoid Protocols
ISBN: 9780896034389 / Angielski / Twarda / 434 str. Termin realizacji zamówienia: ok. 5-8 dni roboczych. Interest in retinoic acid, the main biologically active derivative of vi- min A or retinol, increased dramatically between 1989 and 1993, following the cloning of nuclear receptors or RARs reported in 1987 (Fig. 1). Important discoveries since then have shown how RARs work as all-trans retinoic ac- dependent heterodimers with related nuclear receptors for 9-cis retinoic acid called RXRs. This has stimulated the development of synthetic analogs s- cific for each type of receptor, and opens the way to develop new methods for regulating pharmacologically the activity ofretinoic acid-dependent...
Interest in retinoic acid, the main biologically active derivative of vi- min A or retinol, increased dramatically between 1989 and 1993, following th...
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cena:
845,61 |
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Protein Phosphatase Protocols
ISBN: 9780896034686 / Angielski / Twarda / 316 str. Termin realizacji zamówienia: ok. 5-8 dni roboczych. A major mechanism by which cells regulate protein function is to place phosphate groups on serine and threonine residues. Though the steady-state level of protein phosphorylation depends on the relative activities of both kinases and phosphatases, a much greater effort has previously gone into the study of the former that the latter . Today, however, there is an increasing appreciation for the role that protein phosphatases play in the dynamic p- cess of protein phosphorylation . To date, there are four major types of protein serine/threonine phosphatase catalytic subunits, designated protein...
A major mechanism by which cells regulate protein function is to place phosphate groups on serine and threonine residues. Though the steady-state leve...
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cena:
547,14 |
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PCR in Bioanalysis
ISBN: 9780896034976 / Angielski / Twarda / 281 str. Termin realizacji zamówienia: ok. 5-8 dni roboczych. Offers powerful PCR-based protocols and assays in actual use or potential use in clinical medicine and commercial biology. The main focus of the book is on the commercial applications of PCR, as opposed to basic research uses. Topics covered include: the measurement of hormone levels using PCR; transcription factor isolation; detection of viruses using PCR; detection of tumour contamination of stem cells; and evaluation of grafts for tumour cells.
Offers powerful PCR-based protocols and assays in actual use or potential use in clinical medicine and commercial biology. The main focus of the book ...
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cena:
412,92 |
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2-D Proteome Analysis Protocols
ISBN: 9780896035249 / Angielski / Twarda / 601 str. Termin realizacji zamówienia: ok. 5-8 dni roboczych. With the completion of sequencing projects and the advancement of a- lytical tools for protein identification, proteomics the study of the expressed part of the genome has become a major region of the burgeoning field of functional genomics. High-resolution 2-D gels can reveal virtually all p- teins present in a cell or tissue at any given time, including posttranslationally modified proteins. Changes in the expression and structure of most cellular proteins caused by differentiation or external stimuli can be displayed and eventually identified using 2-D protein gels. 2-D Proteome Analysis...
With the completion of sequencing projects and the advancement of a- lytical tools for protein identification, proteomics the study of the expressed p...
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cena:
619,41 |
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Protein Lipidation Protocols
ISBN: 9780896035348 / Angielski / Twarda / 240 str. Termin realizacji zamówienia: ok. 5-8 dni roboczych. It is hard to think of a protein in eukaryotic cells that does not undergo some type of posttranslational modification. The covalent attachment of l- ids to proteins, protein lipidation, occurs for a few thousand proteins. Several functions for protein lipidation are known. Protein lipids may target proteins to specific cellular membranes, they may serve as molecular switches that allow cytosol-to-membrane transfer, they may direct protein-protein compl- ation, and they may stabilize protein structure. In cases such as the fatty a- lation of intracellular loops of transmembrane proteins, the...
It is hard to think of a protein in eukaryotic cells that does not undergo some type of posttranslational modification. The covalent attachment of l- ...
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cena:
619,41 |
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Stress Response: Methods and Protocols
ISBN: 9780896036116 / Angielski / Twarda / 488 str. Termin realizacji zamówienia: ok. 5-8 dni roboczych. Mammalian cells have evolved a complex multicomponent machinery that enables them to sense and respond to a wide variety of potentially toxic agents present in their environment. These stress responses are often associated with an increased cellular capacity to tolerate normally lethal levels of an insult. The realization that the mammalian stress response may be intimately linked with many human diseases, including rheumatoid arthritis, ischemia, fever, infection, and cancer, has led to an explosion of interest in this research area. Stress Response: Methods and Protocols brings together a...
Mammalian cells have evolved a complex multicomponent machinery that enables them to sense and respond to a wide variety of potentially toxic agents p...
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cena:
547,14 |
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Genomic Imprinting: Methods and Protocols
ISBN: 9780896037410 / Angielski / Twarda / 377 str. Termin realizacji zamówienia: ok. 5-8 dni roboczych. Genomic imprinting is the process by which gene activity is regulated according to parent of origin. Usually, this means that either the maternally inherited or the paternally inherited allele of a gene is expressed while the opposite allele is repressed. The phenomenon is largely restricted to mammals and flowering plants and was first recognized at the level of whole genomes. Nuclear transplantation experiments carried out in mice in the late 1970s established the non-equivalence of the maternal and paternal genomes in mammals, and a similar conclusion was drawn from studies of interploidy...
Genomic imprinting is the process by which gene activity is regulated according to parent of origin. Usually, this means that either the maternally in...
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cena:
412,92 |
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Steroid Receptor Methods: Protocols and Assays
ISBN: 9780896037540 / Angielski / Twarda / 381 str. Termin realizacji zamówienia: ok. 5-8 dni roboczych. This volume of the Methods in Molecular Biology series is entirely devoted to the study of steroid receptor biology. Steroid hormone receptors represent a powerful system for the study of both the most fundamental molecular mec- nisms of gene regulation and control and the gross physiological responses of organisms to steroid hormones. Research in this field has brought forth advances in the treatment of cancer, endocrine disorders, and reproductive biology, and allowed elucidation of the fundamental biological mechanisms of gene expr- sion. In Steroid Receptor Methods: Protocols and Assays,...
This volume of the Methods in Molecular Biology series is entirely devoted to the study of steroid receptor biology. Steroid hormone receptors represe...
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cena:
547,14 |
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DNA Arrays: Methods and Protocols
ISBN: 9780896038226 / Angielski / Twarda / 264 str. Termin realizacji zamówienia: ok. 5-8 dni roboczych. Microarray technology provides a highly sensitive and precise te- nique for obtaining information from biological samples, with the added advantage that it can handle a large number of samples simultaneously that may be analyzed rapidly. Researchers are applying microarray technology to understand gene expression, mutation analysis, and the sequencing of genes. Although this technology has been experimental, and thus has been through feasibility studies, it has just recently entered into widespread use for advanced research. The purpose of DNA Arrays: Methods and Protocols is to provide...
Microarray technology provides a highly sensitive and precise te- nique for obtaining information from biological samples, with the added advantage th...
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cena:
547,14 |
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Two-Hybrid Systems
ISBN: 9780896038325 / Angielski / Twarda / 336 str. Termin realizacji zamówienia: ok. 5-8 dni roboczych. Many, if not all, essential biological processes require selective interactions between proteins. Complex signaling systems require sequential, ordered protein-protein interactions at essentially all levels of the signaling cascade. For example, peptide hormones interact with selective membrane receptor proteins, and autophosphorylation of the receptor then recruits other key regulatory proteins that initiate kinase cascades in which each phosphorylation event requires selective recognition of the protein substrate. The ultimate signaling effect, in many cases, is the regulation of RNA...
Many, if not all, essential biological processes require selective interactions between proteins. Complex signaling systems require sequential, ordere...
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cena:
547,14 |
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Biostatistical Methods
ISBN: 9780896039513 / Angielski / Twarda / 216 str. Termin realizacji zamówienia: ok. 5-8 dni roboczych. Leading biostatisticians and biomedical researchers describe many of the key techniques used to solve commonly occurring data analytic problems in molecular biology, and demonstrate how these methods can be used in the development of new markers for exposure to a risk factor or for disease outcomes. Major areas of application include microarray analysis, proteomic studies, image quantitation, genetic susceptibility and association, evaluation of new biomarkers, and power analysis and sample size.
Leading biostatisticians and biomedical researchers describe many of the key techniques used to solve commonly occurring data analytic problems in mol...
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cena:
412,92 |
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Thyroid Hormone Receptors: Methods and Protocols
ISBN: 9780896039957 / Angielski / Twarda / 223 str. Termin realizacji zamówienia: ok. 5-8 dni roboczych. A panel of outstanding investigators surveys and explains the major cutting-edge methods used in thryroid receptor (TR) research and explains their practical experimental details. Described in step-by-step detail to ensure robust experimental results, the techniques presented cover a wide variety of key areas, including TR in development and knockout (mouse and Xenopus), transcriptional regulation by TRs in both cell-free systems and in living cells, and TR mutant analysis of patients. Additional methods provide powerful tools for the isolation of TR-regulated protein complexes, for studying...
A panel of outstanding investigators surveys and explains the major cutting-edge methods used in thryroid receptor (TR) research and explains their pr...
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cena:
547,14 |
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American Indian MtDNA, Y Chromosome Genetic Data, and the Peopling of North America
ISBN: 9780972134910 / Angielski / Miękka / 236 str. Termin realizacji zamówienia: ok. 5-8 dni roboczych. The field of molecular anthropology has grown in recent years with the advent of new methodologies and theoretical assumptions. The field has been particularly insightful in helping understand the initial peopling of North America. The author discusses the field of molecular anthropology and its insights into the peopling of North America, examining in detail the mtDNA and Y chromosome genetic data. Written in a clear, readable fashion, the author gives an overview of the topic for researchers, graduate students, and other professionals who are interested in this exciting new area of inquiry...
The field of molecular anthropology has grown in recent years with the advent of new methodologies and theoretical assumptions. The field has been par...
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cena:
127,62 |
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The Biology and History of Molecular Biology: New Perspectives
ISBN: 9781402002496 / Angielski / Miękka / 262 str. Termin realizacji zamówienia: ok. 5-8 dni roboczych. This book is a collection of papers which reflect the recent trends in the philosophy and history of molecular biology. It brings together historians, philosophers, and molecular biologists who reflect on the discipline's emergence in the 1950's, its explosive growth, and the directions in which it is going. Questions addressed include: (i) what are the limits of molecular biology? (ii) What is the relation of molecular biology to older subdisciplines of biology, especially biochemistry? (iii) Are there theories in molecular biology? (iv) If so, how are these theories structured? (v) What...
This book is a collection of papers which reflect the recent trends in the philosophy and history of molecular biology. It brings together historians,...
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cena:
454,22 |
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Miracles of Our Time: As Revealed in the Complexity of Cellular Life
ISBN: 9781410795304 / Angielski / Miękka / 131 str. Termin realizacji zamówienia: ok. 5-8 dni roboczych. |
cena:
58,73 |
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Miracles of Our Time: As Revealed in the Complexity of Cellular Life
ISBN: 9781414039275 / Angielski / Twarda / 131 str. Termin realizacji zamówienia: ok. 5-8 dni roboczych. |
cena:
114,87 |
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Microarray Methods and Protocols
ISBN: 9781420046656 / Angielski / Miękka / 216 str. Termin realizacji zamówienia: ok. 5-8 dni roboczych. A Step-by-Step Guide to Present and Future Uses of Microarray Technology Microarray technology continues to evolve, taking on a variety of forms. From the spotting of cDNA and the in situ synthesis of oligonucleotide arrays now come microarrays comprising proteins, carbohydrates, drugs, tissues, and cells. With contributions from microarray experts in both academia and industry, Microarray Methods and Protocols is a turn-by-turn roadmap through the processes necessary to perform a successful microarray experiment. ...A Step-by-Step Guide to Present and Future Uses of Microarray Technology Microarray technology continues to evolve,... |
cena:
898,63 |
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Introduction to Molecular Biology, Genomics and Proteomics for Biomedical Engineers
ISBN: 9781420061192 / Angielski / Twarda / 480 str. Termin realizacji zamówienia: ok. 5-8 dni roboczych. Illustrates the Complex Biochemical Relations that Permit Life to Exist It can be argued that the dawn of the 21st century has emerged as the age focused on molecular biology, which includes all the regulatory mechanisms that make cellular biochemical reaction pathways stable and life possible. For biomedical engineers, this concept is essential to their chosen profession. Introduction to Molecular Biology, Genomics, and Proteomics for Biomedical Engineers hones in on the specialized organic molecules in living organisms and how they... Illustrates the Complex Biochemical Relations that Permit Life to Exist It can be argued that the dawn of the 21st |
cena:
954,48 |
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Handbook of Molecular and Cellular Methods in Biology and Medicine
ISBN: 9781420069389 / Angielski / Twarda / 735 str. Termin realizacji zamówienia: ok. 5-8 dni roboczych. Several milestones in biology have been achieved since the first publication of the Handbook of Molecular and Cellular Methods in Biology and Medicine. This is true particularly with respect to genome-level sequencing of higher eukaryotes, the invention of DNA microarray technology, advances in bioinformatics, and the development of RNAi technology. Now in its third edition, this volume provides researchers with an updated tool kit that incorporates conventional as well as modern approaches to tackle biological and medicinal research in the post-genomics era. ...Several milestones in biology have been achieved since the first publication of the Handbook of Molecular and Cellular Methods in Biology a... |
cena:
1066,23 |