Stress Response: Methods and Protocols

ISBN-13: 9780896036116 / Angielski / Twarda / 2000 / 488 str.

Stephen M. Keyse

Opis

Mammalian cells have evolved a complex multicomponent machinery that enables them to sense and respond to a wide variety of potentially toxic agents present in their environment. These stress responses are often associated with an increased cellular capacity to tolerate normally lethal levels of an insult. The realization that the mammalian stress response may be intimately linked with many human diseases, including rheumatoid arthritis, ischemia, fever, infection, and cancer, has led to an explosion of interest in this research area. Stress Response: Methods and Protocols brings together a diverse array of practical methodologies that may be employed to address various aspects of the response of mammalian cells to environmental stress. The p- tocols are carefully described by authors who have both devised and succe- fully employed them, and they represent a mixture not only of well-established techniques, but also new technologies at the leading edge of research. The areas covered include the detection and assay of stress-induced damage, the acti- tion of signal transduction pathways, stress-inducible gene expression, and stress protein function. Although no volume of this size can be comprehensive and the topics covered reflect a personal choice, it is hoped that it will prove of subst- tial interest and use to a wide range of research workers in the field.

Recenzje

"This handbook will be very helpful in this respect for everybody interested in studies on the effects of stress at the cellular level using biochemical and molecular biology methods." -Cell Biology International

Spis treści

Part I. Detection and Assay of Stress-Induced Damage. Identifying and Counting Protein Modifications Triggered by Nitrosative Stress, Prabodh K. Sehajpal and Harry M. Lander. Determination of Carbonyl Groups in Oxidized Proteins, Rodney L. Levine, Nancy Wehr, Joy A. Williams, Earl R. Stadtman, and Emily Shacter. Quantitation of 4-Hydroxynonenal Protein Adducts, Koji Uchida and Earl R. Stadtman. Detection of Oxidative Stress in Lymphocytes Using Dichlorodihydrofluorescein Diacetate, Cecile M. Krejsa and Gary L. Schieven. The Measurement of Protein Degradation in Response to Oxidative Stress, Thomas Reinheckel, Tilman Grune, and Kelvin J. A. Davies. Part II. The Activation of Signal Transduction by Cellular Stress. Analysis of the Role of the AMP-Activated Protein Kinase in the Response to Cellular Stress, D. Grahame Hardie, Ian P. Salt, and Stephen P. Davies. Detection and Activation of Stress-Responsive Tyrosine Kinases, Gary L. Schieven. Detection of DNA-Dependent Protein Kinase in Extracts from Human and Rodent Cells, Yamini Achari and Susan P. Lees-Miller. Expression and Assay of Recombinant ATM, Yael Ziv, Sharon Banin, Dae-Sik Lim, Christine E. Canman, Michael B. Kastan, and Yosef Shiloh. Detection and Purification of a Multiprotein Kinase Complex from Mammalian Cells: IKK Signalsome, Frank Mercurio, David B. Young, and Anthony M. Manning. Methods to Assay Stress-Activated Protein Kinases, Ana Cuenda. Monitoring the Activation of Stress-Activated Protein Kinases Using GAL4 Fusion Transactivators, Chao-Feng Zheng and Li Xu. Use of Kinase Inhibitors to Dissect Signaling Pathways, Ana Cuenda and Dario R. Alessi. The Development and Use of Phospho-Specific Antibodies to Study Protein Phosphorylation, Jeremy P. Blaydes, Borek Vojtesek, Graham B. Bloomberg, and Ted R. Hupp. Peptide Assay of Protein Kinases and Use of Variant Peptides to Determine Recognition Motifs, D. Grahame Hardie. Part III. The Analysis of Stress-Induced Gene Expression. Assaying NF-kB and AP-1 DNA-Binding and Transcriptional Activity, Judith M. Mueller and Heike L. Pahl. Analysis of the Mammalian Heat Shock Response: Inducible Gene Expression and Heat Shock Factor Activity, Anu Mathew, Yanhong Shi, Caroline Jolly, and Richard I. Morimoto. Approaches to Define the Involvement of Reactive Oxygen Species and Iron in Ultraviolet-A Inducible Gene Expression, Charareh Pourzand, Olivier Reelfs, and Rex M. Tyrrell. The Human Immunodeficiency Virus LTR-Promoter Region as a Reporter of Stress-Induced Gene Expression, Michael W. Bate, Sushma R. Jassal, and David W. Brighty. SAGE: The Serial Analysis of Gene Expression, Jill Powell. Analysis of Differential Gene Expression Using the SABRE Enrichment Protocol, Daniel J. Lavery, Phillippe Fonjallaz, Fabienne Fleury-Olela, and Ueli Schibler. UVB-Regulated Gene Expression in Human Keratinocytes: Analysis by Differential Display, Harry Frank Abts, Thomas Welss, Kai Breuhahn, and Thomas Ruzicka. Part IV. Analysis of Stress Protein Function. Heme Oxygenase Activity: Current Methods and Applications, Stefan W. Ryter, Egil Kvam, and Rex M. Tyrrell. Analysis of Molecular Chaperone Activities Using In Vitro and In Vivo Approaches, Brian C. Freeman, Annamieke Michels, Jaewhan Song, Harm H. Kampinga, and Richard I. Morimoto. Analysis of Chaperone Properties of Small Hsp's, Monika Ehrnsperger, Matthias Gaestel, and Johannes Buchner. Analysis of Small Hsp Phosphorylation, Rainer Benndorf, Katrin Engel, and Matthias Gaestel. Analysis of Multisite Phosphorylation of the p53 Tumor-Suppressor Protein by Tryptic Phosphopeptide Mapping, David W. Meek and Diane M. Milne. The Development of Physiological Models to Study Stress Protein Responses, Ted R. Hupp. Index.

Blurb

In is now understood that the response of mammalian cells to a wide variety of potentially toxic agents may be intimately linked with many human diseases, including rheumatoid arthritis, ischemia, fever, infection, and cancer. In Stress Response: Methods and Protocols, Stephen Keyse has assembled a diverse collection of readily reproducible methods devoted to the study of these varied and powerful responses. Written by leading researchers expert in the techniques they describe, these detailed methods cover the detection and assay of stress-induced damage, the activation of a wide range of signal transduction pathways by cellular stress, stress-induced gene expression, and stress protein function. To ensure experimental success, step-by-step guidance is provided for each method, along with details of reagents, equipment, and other requirements. The methods include both well-established techniques and new technologies at the leading edge of research.
Wide ranging and highly practical, Stress Response: Methods and Protocols provides a gold-standard bench manual for today's basic and clinical scientists working to understand how cells and tissues respond during physiological stress and in human disease