Kategoria BISAC: Science >> Life Sciences - Genetics & Genomics

Rapid changes and significant progress have been made in the Agrobacterium field, such as genetically transforming plants for both basic research purposes and agricultural development. In Agrobacterium Protocols, Third Edition, Volumes 1 and 2, a team of leading experts and veteran researchers describe in detail techniques for delivering DNA to plant cells and permanently altering their genomes. This edition emphasizes agricultural crops and plant species with economic values, with updated protocols on 32 plant species and protocols involving 19 new species. Together...

Several different transformation techniques have been developed over the years and readily shown to be decisive methods in fungal biotechnology. This book will cover the basics behind the most commonly used transformation methods, as well as associated tools and techniques. Each chapter will provide protocols along with examples used in laboratories worldwide. ​

Not only will this text provide a detailed background on applications in industrial and pharmaceutical relevant microbes, but also the importance of fungal pathogens in agricultural production (Phytophthora and...

Genetically Engineered Plants as a Source of Vaccines Against Wide Spread Diseases: An Integrated View provides an integrated outlook of the disciplines involved in the development of plant-based vaccines as well as an updated compilation of the successful developments in the field. The volume covers immunological aspects of mucosal vaccine design, molecular approaches to attain high levels of the recombinant antigens, the rationale of using bioreactor to expand plant biomass, and pharmaceutical technology approaches that have been applied to the development of plant-based vaccine...

This book is really helpful for someone who wants to start learning about genes and DNA. It is a well-written book describing all the introductory materials one would need to become current with genomes and genomics topics. It begins with an introduction to DNA and genes in chapter 1 and goes on from there through epigenetic in chapter 2, including acetylation, methylation, ubiquitylation of protein, deimination, and proline isomerization. It goes through gene editing in chapter 3, which includes good description of TALENs, ZFNs, and CRISPR/Cas systems. Chapter 4 includes cloning using...

This book, written by experienced geneticists, covers topics ranging from the natural history of the mouse species, its handling and reproduction in the laboratory, and its classical genetics and cytogenetics, to modern issues including the analysis of the transcriptome, the parental imprinting and X-chromosome inactivation. The strategies for creating all sorts of mutations, either by genetic engineering or by using mutagens, are also reviewed and discussed in detail. Finally, a last chapter outlines the methodology used for the analysis of complex or quantitative traits.

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Electroporation gene therapy, or gene electrotransfer, has evolved greatly over the last few decades as a result of the remarkable progress in genetic sequencing, gene array analysis, gene cloning, gene expression detection, DNA manufacture and discovery and synthesis of siRNA. Electroporation Protocols: Preclinical and Clinical Gene Medicine, Second Edition provides in-depth knowledge on the delivery of naked DNA and small-interfering RNA (siRNA) to the targeted cells, tissues, and animals for prevention and treatment of disease. It builds on the success of the first edition and on...

Endocrine Biomarkers: Clinical Aspects and Laboratory Determination covers all the pre-analytical variables that can affect test results, both in the clinic and laboratory. Biomarkers of endocrine and bone diseases are discussed from both clinical and laboratory perspectives, and the authors elaborate on the teamwork-based app+roach between the clinician and the laboratory professional in the diagnosis and management of endocrine and bone disorders.

Discussions include test utilization, laboratory measurement methods, harmonization and standardization, interpretation of...

Directed Evolution Library Creation: Methods and Protocols, Second Edition presents user-friendly protocols for both proven strategies and cutting-edge approaches for the creation of mutant gene libraries for directed evolution. As well as experimental methods, information on current computational approaches is provided in a user-friendly format that will allow researchers to make informed choices without needing to comprehend the full technical details of each algorithm. Directed evolution has become a fundamental approach for engineering proteins to enhance activity and explore...

This volume presents a list of cutting-edge protocols for the study of CRISPR-Cas defense systems and their applications at the genomic, genetic, biochemical and structural levels. CRISPR: Methods and Protocols guides readers through techniques that have been developed specifically for the analysis of CRISPR-Cas and techniques adapted from standard protocols of DNA, RNA and protein biology. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents,...

Since the publication of the pioneering first edition of Chemical Genomics and Proteomics more than seven years ago, the area of chemical genomics has rapidly expanded and diversified to numerous novel methods and subdisciplines, such as chemical glycomics and lipidomics. This second edition has been updated to uniquely reflect this interdisciplinary feature as well as the remarkable developments that have occurred. The new edition also covers innovative applications from cell biology to drug discovery to, more recently, clinical diagnostics and medical practice, which utilize the...

PCR Guru: An Ultimate Benchtop Reference for Molecular Biologists is provides researchers in molecular biology with a handy reference for approaching and solving challenging problems associated with PCR setup and optimization. As a laboratory guide, it emphasizes the technical aspects of employing PCR as a tool in molecular biology laboratories. The book covers the history of PCR and the basic science underlying it. It then discusses PCR at the bench level, starting with detailed description and tips on primer design, and continuing with the standard protocols used to perform...