Agrobacterium Protocols: Volume 1

ISBN-13: 9781493945412 / Angielski / Miękka / 2016 / 368 str.

Kan Wang

Opis

Rapid changes and significant progress have been made in the Agrobacterium field, such as genetically transforming plants for both basic research purposes and agricultural development. In Agrobacterium Protocols, Third Edition, Volumes 1 and 2, a team of leading experts and veteran researchers describe in detail techniques for delivering DNA to plant cells and permanently altering their genomes. This edition emphasizes agricultural crops and plant species with economic values, with updated protocols on 32 plant species and protocols involving 19 new species. Together with the 1^st and 2^nd editions, these two volumes offer Agrobacterium-mediated genetic transformation protocols for a total of 76 plant species. For a number of important plants such as rice, barley, wheat and citrus, multiple protocols using different starting plant materials for transformation are included. Volume 1 details updated techniques available for 18 plant species drawn from cereal crops, legume plants, vegetable plants, and three model plant species: Brachypodium distachyon, Medicago truncatula, and Setaria viridis. It also updates a chapter for vector construction, a step critical to a successful plant transformation process. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Agrobacterium Protocols, Third Edition facilitates the transfer of this rapidly developing technology to all researchers for use in both fundamental and applied biology.

Spis treści

Part I

Vectors And Model Plants

1

Gateway^® Compatible Plant Transformation Vectors

 

 

Mark A. Smedley and Wendy A. Harwood

2

Brachypodium Distachyon

 

 

Jennifer N. Bragg, Amy Anderton, Rita Nieu, And John P. Vogel

3

Medicago Truncatula Transformation Using Cotyledonary Explants

 

 

Elane Wright and Zeng-Yu Wang

4

Medicago Truncatula Transformation Using Leaf Explants

 

 

Viviane Cosson, Alexis Eschstruth, and Pascal Ratet

5

Setaria Viridis

 

 

Joyce Van Eck And Kerry Swartwood

 

 

Part II

Cereal Plants

6

Barley (Hordeum Vulgare L.) Transformation Using Immature Embryos

 

 

 

Cornelia Marthe, Jochen Kumlehn and Goetz Hensel

 

7

Barley (Hordeum Vulgare L.) Transformation Using Embryogenic Pollen Cultures

 

 

 

Ingrid Otto, Andrea Müller, and Jochen Kumlehn

 

8

Maize (Zea Mays L.)

 

 

 

Bronwyn Frame, Katey Warnberg, Marcy Main, and Kan Wang

 

9

Maize, Tropical (Zea Mays L.)

 

 

 

Shireen K. Assem

 

10

Finger Millet [Eleusine Coracana (L.) Gaertn]

 

 

 

Stanislaus Antony Ceasar, and Savarimuthu Ignacimuthu

 

11

Oat (Avena Sativa L.)

 

 

 

Sebastian Gasparis and Anna Nadolska-Orczyk

 

12

Rice, Indica (Oryza Sativa L.)

 

 

 

Yukoh Hiei, Yuji Ishida and Toshihiko Komari

 

13

Rice, Japonica (Oryza Sativa L.)

 

 

 

Marcy Main, Bronwyn Frame And Kan Wang

 

14

Sorghum (Sorghum Bicolor L.)

 

 

 

Yuechun Wan And Jeanne Layton

 

15

Wheat (Triticum Aestivum L.) Transformation Using Immature Embryos

 

 

 

Yuji Ishida, Masako Tsunashima, Yukoh Hiei And Toshihiko Komari

 

16

Wheat (Triticum Aestivum L.) Transformation Using Mature Embryos

 

 

 

Eva Medvecká And Wendy A Harwood

 

 

 

Part III

Legume Plants

17

Alfalfa (Medicago Sativa L.)

 

 

Chunxiang Fu, Timothy Hernandez, Chuanen Zhou, and Zeng-Yu Wang

18

Clovers (Trifolium Spp.)

 

 

Samira Rahimi-Ashtiani, Sareena Sahab, Stephen Panter, John Mason, and German Spangenberg

19

Clover, Red (Trifolium Pratense)

 

 

Michael L. Sullivan and Kenneth H. Quesenberry

20

Cowpea [Vigna Unguiculata (L.) Walp]

 

 

Ratikanta Behura, Sanjeev Kumar, Bedabrata Saha, Manas Kumar Panda, Mohitosh Dey, Ayan Sadhukhan, Sagarika Mishra, Shamsher Alam, Debee Prasad Sahoo, Twinkle Sugla, Sanjib Kumar Panda, and Lingaraj Sahoo

22

Lentil (Lens Culinaris Medik.)

 

 

Ufuk Celikkol Akcay, Meral Yücel, Hüseyin Avni Öktem

22

Soybean [Glycine Max (L.) Merr.]

 

 

Diane Luth, Katey Warnburg, and Kan Wang

 

 

Part IV

Legume Plants

23

Brassica Oleracea And B. Napus

 

 

Penny A.C. Hundleby Nee Sparrow, and Judith A. Irwin

24

Cucumber (Cucumis Sativus L.) and Kabocha Squash (Cucurbita Moschata Duch)

 

 

Yoshihiko Nanasato and Yutaka Tabei

25

Pepper, Chili (Capsicum Annuum)

 

 

Jung Min, Sun Hee Shin, En Mi Jeon, Jung Mi Park, Ji Young Hyun,and  Chee Hark Harn

26

Pepper, Sweet (Capsicum Annuum)

 

 

Iris Heidmann And Kim Boutilier

27

Sugar Beet (Beta Vulgaris L.)

 

 

Hiroyo Kagami, Masayuki Kurata, Hiroaki Matsuhira, Kazunori Taguchi, Tetsuo Mikami, Hideto Tamagake, and Tomohiko Kubo

28

Tomato (Solanum Lycopersicum)

 

 

Martha L. Orozco-Cárdenas, Dora Garcia, and Javier Narváez-Vásquez