Genetic Transformation Systems in Fungi, Volume 2

ISBN-13: 9783319364605 / Angielski / Miękka / 2016 / 270 str.

Marco a. Van Den Berg; Karunakaran Maruthachalam

Opis

Not only do they host a range of versatile enzymes used in industrial applications (biofuels, laundry, food processing), as well do they produce several very important pharmaceutical drugs (statins and penicillins). This book will cover the basics behind the most commonly used transformation methods, as well as associated tools and techniques.

Spis treści

I.                   Endogenous DNA: Cell Fusion

1.      Anastomosis and Heterokaryon Formation

Martin Weichert and André Fleißner

2.      Induction of the Sexual Cycle in Filamentous Ascomycetes

Jos Houbraken and Paul S. Dyer

3.      What Have We Learned by Doing Transformations in Neurospora tetrasperma?

Durgadas P. Kasbekar

II.                Endogenous DNA: Repetitive Elements

4.      Repeat-induced Point Mutation: A Fungal-specific, Endogenous Mutagenesis Process

James K. Hane, Angela H. Williams, Adam P. Taranto, Peter S. Solomon and Richard P. Oliver

5.      Calculating RIP Mutation in Fungal Genomes Using RIPCAL

James K. Hane

6.      Fungal Transposable Elements

Linda Paun and Frank Kempken

7.      In vivo Targeted Mutagenesis in Yeast using TaGTEAM

Shawn Finney-Manchester and Narendra Maheshri

III.             Endogenous DNA: RNAi

8.      RNA Silencing in Filamentous Fungi: from Basic to Applications

Nguyen Bao Quoc and Hitoshi Nakayashiki

9.      RNAi-mediated Gene Silencing in the Beta-lactam Producers Fungi Penicillium chrysogenum and Acremonium chrysogenum

Carlos García-Estrada and Ricardo V. Ullán

10.  Controlling Fungal Gene Expression Using the Doxycycline-Dependent Tet-ON System in Aspergillus fumigatus

Michaela Dümig and Sven Krappmann

IV.             Tools & Applications: Selection Markers and Vectors

11.  Expanding the Repertoire of Selectable Markers for Aspergillus Transformation

Khyati Dave, V. Lakshmi Prabha, Manmeet Ahuja, Kashyap Dave, S. Tejaswini and Narayan S. Punekar

12.  Arginase (agaA) as a Fungal Transformation Marker

Kashyap Dave, Manmeet Ahuja, Jayashri, T.N., Rekha Bisht Sirola, Khyati Dave and Narayan S. Punekar

13.  Transformation of Ascomycetous Fungi using Autonomously Replicating Vectors

Takeo Shimizu and Satoko Kanematsu

14.  A Recyclable and Bidirectionally Selectable Marker System for Transformation of Trichoderma

Thiago M. Mello-de-Sousa, Robert L. Mach and Astrid R. Mach-Aigner

15.  Split Marker-Mediated Transformation and Targeted Gene Disruption in Filamentous Fungi

Kuang-Ren Chung and Miin-Huey Lee

V.                Tools & Applications: High Throughput Experimentation

16.  Integrated Automation for Continuous High-Throughput Synthetic Chromosome Assembly and Transformation to Identify Improved Yeast Strains for Industrial Production of Biofuels and Bio-based Chemicals

Stephen R. Hughes and Steven B. Riedmuller

17.  Imaging Flow Cytometry and High-throughput Microscopy for Automated Macroscopic Morphological Analysis of Filamentous Fungi

Aydin Golabgir, Daniela Ehgartner, Lukas Neutsch, Andreas E. Posch, Peter Sagmeister and Christoph Herwig

18.  Yeast Cell Electroporation in Droplet-Based Microfluidic Chip

Qiuxian Cai and Chunxiong Luo

19.  Identification of T-DNA Integration Sites: TAIL-PCR and Sequence Analysis

Jaehyuk Choi, Junhyun Jeon and Yong-Hwan Lee

VI.             Tools & Applications: Comprehensive Approaches in Selected Fungi

20.  Genetic and Genomic Manipulation in Aspergillus niger

Adrian Tsang, Annie Bellemare, Corinne Darmond and Janny Bakhuis

21.  Genetic Manipulation of Meyerozyma guilliermondii

Nicolas Papon,Yuriy R. Boretsky, Vincent Courdavault, Marc Clastre and Andriy A. Sibirny

O autorze

Marco A. van den Berg, PhD

Principal Scientist Applied Biochemistry and Screening

DSM Biotechnology Center

Delft, The Netherlands

 Karunakaran Maruthachalam, PhD

Current: Research Scientist

Global Marker Technology Lab, DuPont-Pioneer

DuPont Knowledge Center

Andhra Pradesh, India

 Previous: Molecular Biologist

California Seed and Plant Labs

Pleasant Grove, CA, USA

Blurb

Several different transformation techniques have been developed over the years and readily shown to be decisive methods in fungal biotechnology. While Volume 1 provided its readers with an in-depth understanding of how the transformation of DNA is used to understand the genetic basis behind these fungal traits, Volume 2 of this text describes the transformation associated methods, tools, and many relevant elements available to the modern fungal researcher.

With ample illustrative examples and protocols, Genetic Transformation Systems in Fungi, Volume 2 is meant not only as a reference guide for the experienced researcher, but also as an a learning tool for the emerging scientist.