Kategoria BISAC: Medical >> Genetics

One Hundred Years of Chromosome Research: What Remains to be Learned, offers the reader a critical analysis of the observations and experiments that shaped the last 100 years of chromosome research, as well as the ideas which prevailed during this period.
Emphasis is placed on what remains to be learned, particularly in light of reality of the sequencing of DNA which leaves the previous era of chromosome research as a prehistoric event. It is at this turning point, that well formulated questions can be asked about many of the chromosome's properties, which remain to...

Since the discovery of microRNAs, developmental biologists have striven to understand the role of miRNAs in development and disease. MicroRNAs in Development: Methods and Protocols collects contributions from expert researchers in order to provide practical guidelines to this complex study. Divided into three convenient sections, this detailed volume covers various techniques to detect and profile miRNA expression, followed by protocols to manipulate the activity of miRNAs in various organisms, and it concludes with a section that outlines different methods to identify and validate...

RNA interference has become a key method in the suppression of gene expression and the development of therapeutic agents, yet there is still the problem of delivery, stability, and the danger of off-target effects such as the silencing of unwanted genes and activation of innate immunity. In siRNA and miRNA Gene Silencing: From Bench to Bedside, expert researchers explore the most recent advances in siRNA design, expression, delivery, in vivo imaging, and methods to minimize siRNA s unwanted effects and promote successful use in patients. As part of the highly successful...

Non-coding, inhibitory microRNAs have emerged as important modulators of cellular gene expression, through a process called RNA interference (RNAi). To date, hundreds of conserved and species-specific microRNAs have been identified in organisms ranging from single-celled algae to humans. Many of these tiny RNAs are now known to play fundamental roles in developmental biology and disease pathogenesis. In addition, RNAi has emerged as a technology useful for manipulating gene expression. In "RNA Interference Techniques," expert researchers present detailed methods for designing and...

Series:

- Highly practical and clinically relevant

- Ophthalmology series is indispensable for continuous education and advanced training

- All editors with international reputation and contributing authors with expertise in their topics

- Reader-friendly format: Well-structured text and design, quick and easy to read

- Richly illustrated with numerous tables and color photos

- Bridges the gap between primary literature and daily practice

- Every 2^nd year each subject is refreshed with timely information about the new...

Pyrosequencing(R) Protocols presents detailed protocols for the multidisciplinary application of Pyrosequencing(R) technology, all written by world-renowned experts in the field. This comprehensive volume enables quick reference by collecting the primary applications for Pyrosequencing(R), and supplementing each protocol with troubleshooting tips specific to that method.

Chapters 1 and 2 present an introductory overview of the origins of the Pyrosequencing(R) methodology and its key applications. Chapters 3 and 4 describe primer selection and the basic Pyrosequencing(R) technique,...