This volume brings together expert contributors to present the development of recent genetic techniques that allow for generating genetically encoded activity sensors in order to investigate neuronal activity. Each chapter describes a specific sensor and its utilization to study neuronal activity in a particular way.
This volume brings together expert contributors to present the development of recent genetic techniques that allow for generating genetically encoded ...
Advances in methodologies and experimental models are pivotal to furthering our understanding of central nervous system (CNS) functions in mammals. Isolated Central Nervous System Circuits examines the application a variety of technologies to mostly active in vitro preparations from basically different CNS regions with a diversity of functions. From patch-clamp, multiphoton imaging, live fluorescent protein expression, or immunohistochemistry techniques to acute or 'organotypic' slices and en bloc preparations, this detailed volume features key protocols and examples...
Advances in methodologies and experimental models are pivotal to furthering our understanding of central nervous system (CNS) functions in mammals....
This combined analysis of the environmental, genetic and social causes of eating disorders includes lab-ready protocols as well as contributions from authors well versed in deploying the methodologies. It is a vital new volume in the Neuromethods series.
This combined analysis of the environmental, genetic and social causes of eating disorders includes lab-ready protocols as well as contributions from ...
Since its first application, microdialysis has become incredibly popular to study brain function and has been applied with success in different fields from psychopharmacology, neurobiology, and physiology in animals and also humans. Microdialysis Techniques in Neuroscience focuses on the practical aspects of microdialysis in animal and human, highlighting current technical limitations and providing a vision of what is yet to come for the determination of the most disparate compounds in the brain. The book's contents range from new techniques for detection and quantifying the release...
Since its first application, microdialysis has become incredibly popular to study brain function and has been applied with success in different fie...
The development of treatment strategies that can help patients with spinal cord injury to regain lost functions and an improved quality of life is a major medical challenge, and experimental spinal cord research has to meet these challenges by resolving fundamental problems, establishing a basis for possible novel treatment strategies of spinal cord injury, and motivating their clinical translation. In Animal Models of Spinal Cord Repair, expert researchers examine a broad range of experimental models for research on spinal cord injury, how they have contributed to our current state...
The development of treatment strategies that can help patients with spinal cord injury to regain lost functions and an improved quality of life is ...
Although the available models, whether at the cellular, tissue, or animal level, do not exactly represent the biology of human brain tumors, animal models can offer significant insights into these tumors, providing a better understanding of biological mechanisms underlying tumor generation, growth, angiogenesis, invasion, and metastasis. Animal Models of Brain Tumors brings together developments and discoveries in "in vivo" experimental tumor research that have provided advances in our understanding of the cellular and molecular mechanisms involved in the generation, progression,...
Although the available models, whether at the cellular, tissue, or animal level, do not exactly represent the biology of human brain tumors, animal...
Since the introduction of fluorescent calcium indicators and the subsequent development of capacities for real-time monitoring and imaging of calcium movements in the intact cells studied in isolation, in situ and in vivo, the complex and vital calcium signaling system has been illuminated, proving calcium signals to be excellent universal reporters of cellular activity. In "Calcium Measurement Methods," a team of renowned experts in the field contribute methods dedicated to calcium imaging in neural cells with a balance between the main principles of calcium imaging and specific...
Since the introduction of fluorescent calcium indicators and the subsequent development of capacities for real-time monitoring and imaging of calci...
Synapses underlie rapid and flexible neural communication in the brain and they hold the key to understanding higher brain functions in health and disease. Because they are very small and highly dynamic, it is very difficult to study them with traditional techniques. Fortunately, recent ground-breaking advances in optical microscopy (e.g. STED, PALM, STORM, SIM) have greatly improved our ability to image living synapses at the nanoscale, even down to the level of single molecules. The proposed volume brings together leading researchers to review these exciting new techniques and their...
Synapses underlie rapid and flexible neural communication in the brain and they hold the key to understanding higher brain functions in health and dis...
This second edition provides updated and expanded chapters that critically address the issues or rodent stroke modeling, from choosing the model and outcome measures, designing the experiment, conducting and analyzing it, to reporting it in a scientific publication. Rodent Models of Stroke, Second Edition aims to help its readers understand the limitations and the opportunities of modeling stroke in rodents and enable them to conduct experiments which will not only improve our understanding of the pathophysiology of this devastating disorder but also serve as the basis for...
This second edition provides updated and expanded chapters that critically address the issues or rodent stroke modeling, from choosing the model an...
The second edition of this volume provides up-to-date methods on the main methodological aspects of functional MRI (fMRI), applying fMRI to the study of central nervous system, and future evolutions of fMRI techniques. fMRI: Method and Protocols, Second Edition guides the reader through chapters on basic knowledge for the understanding of the technical aspects of fMRI, overview of the main results derived from the application of fMRI to the study of healthy individuals, application of fMRI to assess the role of brain plasticity in the major neurological and psychiatric conditions,...
The second edition of this volume provides up-to-date methods on the main methodological aspects of functional MRI (fMRI), applying fMRI to the stu...
