This book deals primarily with the principal extracellular macromole cules of animal connective tissues. It attempts to answer some general questions about the biological organization of the tissues: What is the nature of this organization at various dimensional levels? What functions does the organization serve? How has it evolved? I have given major emphasis to the structures and properties of the macromolecular components of extracellular matrices from a wide range of invertebrates and vertebrates. In doing so, however, I have treated cursorily many important aspects of connective tissue...

This book is based on practical experience and is therefore written as a prac tical manual. The fore-runners of the book were the manuals of the first and second EMBO-Courses on "Micromethods in Molecular Biology" which were held in G6ttingen in the spring of 1970 and the autumn of 1971. This book may serve as a manual not only for the participants of the third EMBO-Course to be held in G6ttingen in autumn 1973, but also for all experimenters who are interested in using micromethods. It must be emphasized from the outset that this book is conceived as a "cook book" and not as a monograph...

The application of circular dichroism (CD) to various problems involving con formation of proteins and other biopolymers is emphasized in this revised and enlarged second edition. The usefulness of CD and ORD in helping to solve structural prob lems is demonstrated by many examples, and the most essential data are tabulated. The author is sincerely grateful to the editors of the series Molecular Biology, Bio chemistry and Biophysics, especially to Professor GEORG F. SPRINGER, M.D., for their interest in this edition, as well as to the many reviewers for their constructive criticism of the...

The purpose of this monograph is to bring together under one cover results of research on phenomena drawn from the fields of chemistry, biochemistry, bio physics, virology, and cell biology. The processes and reactions considered have one important feature in common: they are endothermic and, therefore, entropy driven. They are, in the main, reversible reactions leading to the formation of large structures, some of which play critical roles in life processes. If one thinks only of the subunits and of the structures they form upon poly merization, it seems to be a contradiction that such...

Studies on microbial RNases began in 1924 when NOGUCHI found nucleic acid degrading enzymes in Takadiastase, a commercially prepared digest from Aspergillus oryzae. In 1935 OrANI reported the presence of enzymes degrading yeast RNA in fungi (Aspergillus sp. etc.). In 1948, MCCARTY studied the nuclease activity in 36 strains of group A hemolytic Streptococci and indicated the release of both RNase and DNase from Streptococcal cells during growth in culture media. As is well known, these nucleases (TILLETT et al., 1948) are applied in the treatment of inflammatory exudates. In 1952, MUGGLETON...