Prion diseases, also known as Transmissible Spongiform Encephalopathies (TSEs), exist in both humans (Creutzfeldt-Jakob disease (CJD)) and animals (scrapie, bovine spongiform encephalopathy (BSE), chronic wasting disease) and have the unique property of being infectious, sporadic or genetic in origin.
Although the precise nature of the infectious agent responsible for TSEs is not definitely identified, it is now clearly demonstrated that a protein named PrP (for Prion Protein) plays a critical role in the transmission and pathogenesis of TSEs.
This book provides the general...
Prion diseases, also known as Transmissible Spongiform Encephalopathies (TSEs), exist in both humans (Creutzfeldt-Jakob disease (CJD)) and animals ...
The technique of needle microinjection has become a prominent experime ntal approach in biological research. Cellular organelles, DNA and RNA, enzymes, structural proteins, metabolites, ions and antibodies are j ust some of the molecular and cellular elements that have been transpo sed from test tubes into living cells by needle injection. This techni que is broadly used as a valuable tool for the study of many different cell responses in a variety of systems. From individual cell signalin g responses, such as regulation of metabolic pathways and second funct ion of second messengers, the...
The technique of needle microinjection has become a prominent experime ntal approach in biological research. Cellular organelles, DNA and RNA, enzymes...
It is now something of a truism to say that natural toxins form a major compo nent of the molecular tools used increasingly frequently by the ever growing number of laboratories of various kinds. Evidence for this is provided not only by the increasing number of firms including such toxins in their catalogues but also by the large number of demands received by those who discover new tox ins. This book is designed to facilitate the work of scientists interested in explor ing a new domain of toxicology or in using toxins as tools for research. The length of this book has been kept reasonable by...
It is now something of a truism to say that natural toxins form a major compo nent of the molecular tools used increasingly frequently by the ever gro...
A description of the latest and most appropriate mathematical and numerical methods for optimizing soil venting. The monograph considers mathematical, numerical, and technical aspects as well as their practical significance. This book will be of interest to applied mathematicians, geophysicists, geoecologists, soil physicists, and environmental engineers.
A description of the latest and most appropriate mathematical and numerical methods for optimizing soil venting. The monograph considers mathematic...
Analytical Biotechnology presents a set of technologies that provide state-of-the art tools for genomics, proteomics, metabolomics, drug discovery, screening, and analysis of biomolecules such as DNA, proteins, and small metabolites. Thus, the book covers topics from immunoassays and biochips to nano-biochemistry. Moreover, it aims to apply advanced automation and microfabrication technology to the development of biosensor devices as well as integrated systems. This volume promotes cross-disciplinary approaches directed toward solving key problems in biology and medicine. Each chapter...
Analytical Biotechnology presents a set of technologies that provide state-of-the art tools for genomics, proteomics, metabolomics, drug discovery, sc...
Prokaryotic Genomics provides molecular microbiologists in particular and researchers working with bacteria in general with the most important established recipes needed for their work. The volume covers both revisited classical methods and new tools for global analysis such as genomics or proteomics. It is written for those in need of a bench manual to complete their experiments and for those wanting to understand the modern tools used in microbiology.
Prokaryotic Genomics provides molecular microbiologists in particular and researchers working with bacteria in general with the most important establi...
This manual reflects practical approaches to handling bacteria in the labora tory. It is designed to recall historical methods of bacterial genetics that have had recent developments and to present new techniques that allow full genome analysis. It has been written for microbiologists who need to group their protocols at the state of the art of a new millennium and also for scientists in other fields of life sciences who need to use bacteria for their research. Teachers, graduate students, and postdocs also will benefit from having these protocols to help them understand modern bacterial...
This manual reflects practical approaches to handling bacteria in the labora tory. It is designed to recall historical methods of bacterial genetics t...
Modern analytical biotechnology is focused on the use of a set of enabling platform technologies that provide contemporary, state-of-the-art tools for genomics, proteomics, metabolomics, drug discovery, screening, and analysis of natural product molecules. Thus, analytical biotechnology covers all areas of bioanalysis from biochips and nano-chemistry to biology and high throughput screening. Moreover, it aims to apply advanced automation and micro fabrica tion technology to the development of robotic and fluidic devices as well as integrated systems. This book focuses on enhancement...
Modern analytical biotechnology is focused on the use of a set of enabling platform technologies that provide contemporary, state-of-the-art tools for...
One major concern of biotechnology is either using enzymes or producing them. Enzyme/protein production is therefore an important starting point for biotechnology. Bioseparation or Downstream Processing constitutes about 40-90% of the total production cost. Driven by economics, highly selective technologies applicable to large-scale processing have emerged during the last decade. These technologies are slowly diffusing to enzymologists who are working on a smaller scale, looking for fast and efficient purification protocols. The affinity-based techniques (including precipitation, two-phase...
One major concern of biotechnology is either using enzymes or producing them. Enzyme/protein production is therefore an important starting point fo...
