One major concern of biotechnology is either using enzymes or producing them. Enzyme/protein production is therefore an important starting point for biotechnology. Bioseparation or Downstream Processing constitutes about 40-90% of the total production cost. Driven by economics, highly selective technologies applicable to large-scale processing have emerged during the last decade. These technologies are slowly diffusing to enzymologists who are working on a smaller scale, looking for fast and efficient purification protocols. The affinity-based techniques (including precipitation, two-phase...
One major concern of biotechnology is either using enzymes or producing them. Enzyme/protein production is therefore an important starting point fo...
Munishwar Nath Gupta M. N. Gupta Munishwar N. Gupta
One major concern of biotechnology is either using enzymes or producing them. Enzyme/protein production is therefore an important starting point for biotechnology. Bioseparation or Downstream Processing constitutes about 40-90% of the total production cost. Driven by economics, highly selective technologies applicable to large-scale processing have emerged during the last decade. These technologies are slowly diffusing to enzymologists who are working on a smaller scale, looking for fast and efficient purification protocols. The affinity-based techniques (including precipitation, two-phase...
One major concern of biotechnology is either using enzymes or producing them. Enzyme/protein production is therefore an important starting point fo...
Munishwar Nath Gupta M. N. Gupta Munishwar N. Gupta
Extending the range of enzymatic catalysis by using non-aqueous media considerably enlarges the range of enzyme applications and became a po werful approach in biochemistry and biotechnology. This book discusses several specific applications in nearly anhydrous media, aqueous-orga nic co-solvent mixtures and reverse micelles, including use of enzymes in fat and oil processing, peptide synthesis, sugar transformations a nd preparation of chiral compounds. A particularly promising approach of using reverse micelles for modifying proteins is also described.
Extending the range of enzymatic catalysis by using non-aqueous media considerably enlarges the range of enzyme applications and became a po werful ap...
