Methods in Non-Aqueous Enzymology

ISBN-13: 9783764358037 / Angielski / Twarda / 2000 / 218 str.

Munishwar Nath Gupta; M. N. Gupta; Munishwar N. Gupta

Opis

Extending the range of enzymatic catalysis by using non-aqueous media considerably enlarges the range of enzyme applications and became a po werful approach in biochemistry and biotechnology. This book discusses several specific applications in nearly anhydrous media, aqueous-orga nic co-solvent mixtures and reverse micelles, including use of enzymes in fat and oil processing, peptide synthesis, sugar transformations a nd preparation of chiral compounds. A particularly promising approach of using reverse micelles for modifying proteins is also described.

Spis treści

1. Non-Aqueous Enzymology: Issues and Perspectives.- 2. Importance of Water Activity for Enzyme Catalysis in Non-Aqueous Organic Systems.- 3. Engineering of Enzymes via Immobilization and Post-Immobilization Techniques: Preparation of Enzyme Derivatives with Improved Stability in Organic Media.- 4 Immobilization of Lipases for Use in Non-Aqueous Reaction Systems.- 5. Applications of Enzymes and Membrane Technology in Fat and Oil Processing.- 6. Strategies for Improving the Lipase-Catalyzed Preparation of Chiral Compounds.- 7. Peptide Synthesis in Non-Aqueous Media.- 8. Enzyme Selectivity in Organic Media.- 9. Sugar Transformations Using Enzymes in Non-Aqueous Media.- 10. Reversed Micelles as Microreactors: N-Terminal Acylation of RNase A and its Characterization.- 11. Analysis in Non-Aqueous Milieu Using Thermistors.- 12. Importance of the Medium forin vitroandin vivoProtein Folding Mechanisms: Biomedical Implications.