Munishwar Nath Gupta M. N. Gupta Munishwar N. Gupta
One major concern of biotechnology is either using enzymes or producing them. Enzyme/protein production is therefore an important starting point for biotechnology. Bioseparation or Downstream Processing constitutes about 40-90% of the total production cost. Driven by economics, highly selective technologies applicable to large-scale processing have emerged during the last decade. These technologies are slowly diffusing to enzymologists who are working on a smaller scale, looking for fast and efficient purification protocols. The affinity-based techniques (including precipitation, two-phase...
One major concern of biotechnology is either using enzymes or producing them. Enzyme/protein production is therefore an important starting point fo...
Munishwar Nath Gupta M. N. Gupta Munishwar N. Gupta
Extending the range of enzymatic catalysis by using non-aqueous media considerably enlarges the range of enzyme applications and became a po werful approach in biochemistry and biotechnology. This book discusses several specific applications in nearly anhydrous media, aqueous-orga nic co-solvent mixtures and reverse micelles, including use of enzymes in fat and oil processing, peptide synthesis, sugar transformations a nd preparation of chiral compounds. A particularly promising approach of using reverse micelles for modifying proteins is also described.
Extending the range of enzymatic catalysis by using non-aqueous media considerably enlarges the range of enzyme applications and became a po werful ap...
